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Trhr2 (NM_133202) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR215463
Trhr2 (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone receptor 2 (Trhr2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trhr2
Synonyms TRH-R2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215463 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATGGTCCCAGCAATGTCTCACTCGTTCACGGTGACACCACACTGGGCCTCCCAGAGTACAAAGTAG
TCTCGGTCCTCCTGGTCCTCCTGGTGTGCACCGTGGGCATCGTGGGCAATGCCATGGTGGTCCTGGTGGT
GCTGACCTCACGTGACATGCACACACCCACCAACTGCTACCTGGTCAGCCTGGCTCTCGCTGACCTCATT
GTGCTGCTGGCTGCGGGGCTGCCCAATGTCTCTGACAGCCTGGTGGGGCACTGGATCTATGGACATGCTG
GCTGCTTGGGCATCACCTACTTCCAGTACCTAGGCATCAATGTCTCCTCCTGCTCCATCCTGGCCTTCAC
TGTGGAGAGGTACATCGCCATTTGCCACCCAATGAGAGCACAGACCGTGTGCACTGTGGCCCGGGCCAGA
CGGATCATCGCAGGCATTTGGGGGGTCACGTCCCTCTATTGCTTACTCTGGTTCTTTCTGGTGGACCTCA
ATGTCCGTGACAACCAGCGCCTGGAATGTGGCTACAAGGTGTCCCGAGGCCTCTACCTGCCCATCTACCT
GCTGGACTTTGCTGTCTTCTTCATTGCACCCTTGCTGGGGACCCTCGTGCTCTATGGGTTCATTGGAAGG
ATCTTATTTCAGAGCCCATTGTCCCAGGAGGCCTGGCAGAAGGAGAGGCAGTCCCATGGGCAGAGCGAGG
GTACACCAGGCAACTGCTCTAGGTCCAAGAGCTCAATGTCCTCCAGGAAGCAGGCCACCAGGATGCTGGC
CGTGATTGTGTTGCTCTTTGCAGTGCTCTGGACCCCCTACCGCACACTGGTGCTGCTCAACTCCTTCCTG
GCCCGGCCTTTCCTGGACCCCTGGGTCCTGCTGTTCTGCCGCACCTGTGTCTACACCAACAGTGCCATCA
ACCCTGTCATCTACAGCCTCATGTCGCAGAAATTCCGGGCAGCCTTCCTGAAACTGTGCTGGTGCCGGGC
AGCTGGGTCCCAGAGACGTGCAGCATGCGCCCCCACCAGCAACTACAGTGCTGCCCAGGAGACCTCCCTA
GGAACTGAGAAGATGCAACTGGGCTCCCGTGAGGTCTCAGGTCCAGCAGCAGCTGCTTCCCTGCGTTGCC
AGCATGAGCCCCACTTCAGTGTGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215463 protein sequence
Red=Cloning site Green=Tags(s)

MDGPSNVSLVHGDTTLGLPEYKVVSVLLVLLVCTVGIVGNAMVVLVVLTSRDMHTPTNCYLVSLALADLI
VLLAAGLPNVSDSLVGHWIYGHAGCLGITYFQYLGINVSSCSILAFTVERYIAICHPMRAQTVCTVARAR
RIIAGIWGVTSLYCLLWFFLVDLNVRDNQRLECGYKVSRGLYLPIYLLDFAVFFIAPLLGTLVLYGFIGR
ILFQSPLSQEAWQKERQSHGQSEGTPGNCSRSKSSMSSRKQATRMLAVIVLLFAVLWTPYRTLVLLNSFL
ARPFLDPWVLLFCRTCVYTNSAINPVIYSLMSQKFRAAFLKLCWCRAAGSQRRAACAPTSNYSAAQETSL
GTEKMQLGSREVSGPAAAASLRCQHEPHFSVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133202
ORF Size 1146 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133202.1, NM_133202.2, NP_573465.1
RefSeq Size 1340 bp
RefSeq ORF 1149 bp
Locus ID 170732
Cytogenetics 8 E1
MW 42.1 kDa
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"NM_133202" in other vectors (4)
SKU Description Size Price
MC212329 Trhr2 (untagged) - Mouse thyrotropin releasing hormone receptor 2 (Trhr2), (10ug) 10 ug
$503.00
MG215463 Trhr2 (tGFP-tagged) - Mouse thyrotropin releasing hormone receptor 2 (Trhr2), (10ug) 10 ug
$657.00
MR215463L3 Lenti ORF clone of Trhr2 (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone receptor 2 (Trhr2) 10 ug
$757.00
MR215463L4 Lenti ORF clone of Trhr2 (mGFP-tagged) - Mouse thyrotropin releasing hormone receptor 2 (Trhr2) 10 ug
$757.00

Plasmid Map for MR215463

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR215463
Circular map for MR215463

file-alt Citations

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