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Cyp4a12b (NM_172306) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR215319
Cyp4a12b (Myc-DDK-tagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 12B (Cyp4a12b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $474.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cyp4a12b
Synonyms BC060945; Cyp4a12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215319 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCCTCTGCTCTGAGCTCCATCAGATTCCCAGGAAGCATCTCTGAGTACCTTCAAGTAGCCTCTG
TGCTCAGCCTGCTCCTGCTGCTCTTCAAGACAGCCCAGCTCTACCTGCACAGGCAATGGCTACTCAGCAG
TACTCAGCAGTTCCCATCCCCACCTTCTCACTGGCTCTTTGGACACAAGATCTTAAAGGACCAGGACCTT
CAAGATATTCTAACTAGGATTAAGAATTTCCCAAGTGCCTGTCCACAGTGGCTCTGGGGAAGCAAAGTGC
GCATTCAAGTGTATGACCCTGACTACATGAAGCTGATTCTGGGGAGATCAGACCCAAAAGCTCATGGTTC
CTACAGATTTCTAGCTCCCTGGATTGGGCGTGGTTTGCTTCTGCTGGATGGACAGACATGGTTTCAGCAC
CGACGAATGTTGACCCCAGCTTTCCACTATGACATTCTGAAGCCTTACACGGAAATCATGGCAGACTCTG
TTCATGTCATGCTGGATAAATGGGAACAGATTGTTGGCCAGGATTCCACCCTGGAGATCTTCCAACACAT
CACCTTGATGACCTTGGACACCATCATGAAGTGTGCCTTCAGCCATGAGGGCAGTGTCCAGTTGGACAGA
AAATACAAGTCCTATATCCAGGCAGTTGAGGACCTGAACAATCTCTTTTTTCTCCGTGTGCGGAACATCT
TTCACCAGAATGACATCATCTACAGAGTGTCCTCTAATGGCTGCTTGGCCAACAGTGCCTGCCAACTTGC
CCATGATCACACAGACCAAGTGATCAAATCAAGGAGGAGTCAACTTCAGGATGAGGAAGAGTTGGAAAAG
CTTAAGATGAAAAGGCGATTGGATTTCCTGGACATCCTCCTATTTGCCAGAATGGAAAATGGAAAAAGCT
TATCTGATAAGGACCTTCGTGCTGAAGTGGATACTTTCATGTTCGAGGGCCATGACACCACAGCTAGTAG
TATCTCCTGGATCTTCTATGCTTTGGCCACAAATCCTGAACATCAACAGAGATGCAGGAAGGAGATCCAA
AGTCTCTTAGGAGATGGGGCTTCTATCACCTGGAACGACCTGGACAAGATGCCCTATACTACCATGTGCA
TCAAGGAGGCCCTGAGGATCTACCCCCCTGTACCAAGTGTGAGCAGAGAGCTCAGCTCACCTGTCACCTT
TCCTGATGGACGTTCTTTACCCAAAGGTATCCATGTCATGCTCTCCTTTTATGGCCTTCATCACAACCCA
ACTGTGTGGCCAAATCCAGAGGTGTTTGATCCTTCTCGATTTGCACCAGGGTCTTCCCGGCACAGCCACT
CATTCCTGCCCTTCTCAGGAGGAGCAAGGAACTGCATTGGGAAACAGTTTGCGATGAATGAGCTGAAGGT
GGCTGTGGCCCTGACCCTGCTCCGCTTTGAGCTGCTGCCAGATCCCACCAGAGTCCCAATCCCCATACCA
AGAATTGTGTTGAAGTCCAAGAATGGGATCCACTTGCATCTCAAAAAGCTCCAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215319 protein sequence
Red=Cloning site Green=Tags(s)

MSASALSSIRFPGSISEYLQVASVLSLLLLLFKTAQLYLHRQWLLSSTQQFPSPPSHWLFGHKILKDQDL
QDILTRIKNFPSACPQWLWGSKVRIQVYDPDYMKLILGRSDPKAHGSYRFLAPWIGRGLLLLDGQTWFQH
RRMLTPAFHYDILKPYTEIMADSVHVMLDKWEQIVGQDSTLEIFQHITLMTLDTIMKCAFSHEGSVQLDR
KYKSYIQAVEDLNNLFFLRVRNIFHQNDIIYRVSSNGCLANSACQLAHDHTDQVIKSRRSQLQDEEELEK
LKMKRRLDFLDILLFARMENGKSLSDKDLRAEVDTFMFEGHDTTASSISWIFYALATNPEHQQRCRKEIQ
SLLGDGASITWNDLDKMPYTTMCIKEALRIYPPVPSVSRELSSPVTFPDGRSLPKGIHVMLSFYGLHHNP
TVWPNPEVFDPSRFAPGSSRHSHSFLPFSGGARNCIGKQFAMNELKVAVALTLLRFELLPDPTRVPIPIP
RIVLKSKNGIHLHLKKLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172306
ORF Size 1524 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172306.1, NM_172306.2, NP_758510.2
RefSeq Size 2395 bp
RefSeq ORF 1527 bp
Locus ID 13118
UniProt ID A2A974
Cytogenetics 4 D1
MW 58.3 kDa
Summary A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed:17112342). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:17112342). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids (PubMed:17112342). May act as a major omega-hydroxylase for dodecanoic (lauric) acid in kidney (PubMed:17112342). Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:17112342). Acts as an omega-hydroxylase and epoxidase toward (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoc acid (EPA). Catalyzes the epoxidation of the last double bond of EPA with no preferred stereoselectivity, producing both (R,S) and (S,R) stereoisomers (PubMed:17112342). Can also catalyze the omega-1 and omega-2 oxidation of fatty acids with lower efficiency (PubMed:17112342).UniProtKB/Swiss-Prot Function
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"NM_172306" in other vectors (5)
SKU Description Size Price
MC202895 Cyp4a12b (untagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 12B (Cyp4a12b), (10ug) 10 ug
$474.00
MC218751 Cyp4a12b (untagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 12B (Cyp4a12b), (10ug) 10 ug
$521.00
MG215319 Cyp4a12b (tGFP-tagged) - Mouse cytochrome P450 family 4 subfamily a polypeptide 12B (Cyp4a12b), (10ug) 10 ug
$674.00
MR215319L3 Lenti ORF clone of Cyp4a12b (Myc-DDK-tagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 12B (Cyp4a12b) 10 ug
$774.00
MR215319L4 Lenti ORF clone of Cyp4a12b (mGFP-tagged) - Mouse cytochrome P450, family 4, subfamily a, polypeptide 12B (Cyp4a12b) 10 ug
$774.00

Plasmid Map for MR215319

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR215319
Circular map for MR215319

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