Dgkd (NM_177646) Mouse Tagged ORF Clone
$589.00
$1,062.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dgkd |
| Synonyms | AI841987; D330025K09; dgkd-2; DGKdelta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_177646 |
| ORF Size | 3660 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_177646.3, NP_808314.2 |
| RefSeq Size | 5694 bp |
| RefSeq ORF | 3663 bp |
| Locus ID | 227333 |
| UniProt ID | E9PUQ8 |
| Cytogenetics | 1 D |
| MW | 135.2 kDa |
| Summary | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:17021016). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:17021016). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development (PubMed:17021016). May also regulate clathrin-dependent endocytosis (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_177646" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC224002 | Dgkd (untagged) - Mouse diacylglycerol kinase, delta (Dgkd), (10ug) | 10 ug |
$1,169.00
|
|
| MG215309 | Dgkd (tGFP-tagged) - Mouse diacylglycerol kinase delta (Dgkd), (10ug) | 10 ug |
$1,262.00
|
|
| MR215309L3 | Lenti ORF clone of Dgkd (Myc-DDK-tagged) - Mouse diacylglycerol kinase, delta (Dgkd) | 10 ug |
$1,362.00
|
|
| MR215309L4 | Lenti ORF clone of Dgkd (mGFP-tagged) - Mouse diacylglycerol kinase, delta (Dgkd) | 10 ug |
$1,362.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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