Olfr73 (NM_054090) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR215298
Olfr73 (Myc-DDK-tagged) - Mouse olfactory receptor 73 (Olfr73)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr73
Synonyms fascin-1; mOR-EG; MOR174-9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215298 representing NM_054090
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTCTGTCAGATGGAAATCACAGTGGGGCTGTGTTCACCCTCTTGGGCTTCTCAGATTATCCAGAAT
TGACAATCCCTCTCTTTTTGATATTTCTCACCATCTACAGCATCACTGTGGTAGGAAATATTGGCATGAT
TGTCATAATCAGAATTAATCCCAAACTGCACATCCCCATGTACTTCTTTCTCAGCCACCTCTCTTTTGTG
GATTTTTGTTATTCCTCCATTGTTGCTCCCAAGATGCTGGTAAATCTAGTTACAATGAACAGAGGCATAT
CATTTGTAGGATGCTTAGTGCAATTCTTTTTTTTCTGTACTTTCGTGGTAACTGAATCTTTTCTATTAGG
AGTGATGGCTTATGACAGGTTTGTGGCCATCCGCAACCCTCTACTCTACACAGTGGCCATGTCCCAGAGG
CTCTGTGCCATGCTGGTATTGGGATCCTATGCTTGGGGGGTGGTATGCTCCTTGATACTGACCTGCTCTG
CCTTGAATCTTTCTTTTTATGGTTTCAATATGATCAACCACTTTTTTTGTGAGTTCTCCTCTCTCCTTTC
ACTTTCACGCTCTGACACATCTGTCAGTCAACTGTTGCTTTTCGTTTTTGCCACTTTTAATGAGATTAGC
ACACTCCTTATCATTCTCTTGTCTTATGTGCTCATTGTTGTTACTATTCTGAAGATGAAGTCAGCAAGTG
GACGCCGCAAAGCCTTCTCCACTTGTGCTTCCCATCTGACAGCTATAACCATCTTCCATGGAACAATCCT
ATTCCTATACTGTGTACCTAACTCCAAGAACTCCAGGCATACCGTAAAAGTGGCCTCTGTGTTTTACACA
GTGGTGATCCCCATGCTGAATCCCCTAATATACAGTCTGAGAAATAAGGATGTCAAGGACACAGTAAAAA
AAATAATAGGTACTAAAGTCTATTCTTCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR215298 representing NM_054090
Red=Cloning site Green=Tags(s)

MTLSDGNHSGAVFTLLGFSDYPELTIPLFLIFLTIYSITVVGNIGMIVIIRINPKLHIPMYFFLSHLSFV
DFCYSSIVAPKMLVNLVTMNRGISFVGCLVQFFFFCTFVVTESFLLGVMAYDRFVAIRNPLLYTVAMSQR
LCAMLVLGSYAWGVVCSLILTCSALNLSFYGFNMINHFFCEFSSLLSLSRSDTSVSQLLLFVFATFNEIS
TLLIILLSYVLIVVTILKMKSASGRRKAFSTCASHLTAITIFHGTILFLYCVPNSKNSRHTVKVASVFYT
VVIPMLNPLIYSLRNKDVKDTVKKIIGTKVYSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_054090
ORF Size 939 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054090.1, NP_473431.1
RefSeq Size 942 bp
RefSeq ORF 942 bp
Locus ID 117004
Cytogenetics 2 D
MW 35.5 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_054090" in other vectors (4)
SKU Description Size Price
MC212294 Olfr73 (untagged) - Mouse olfactory receptor 73 (Olfr73), (10ug) 10 ug
$450.00
MG215298 Olfr73 (tGFP-tagged) - Mouse olfactory receptor 73 (Olfr73), (10ug) 10 ug
$650.00
MR215298L3 Lenti ORF clone of Olfr73 (Myc-DDK-tagged) - Mouse olfactory receptor 73 (Olfr73) 10 ug
$750.00
MR215298L4 Lenti ORF clone of Olfr73 (mGFP-tagged) - Mouse olfactory receptor 73 (Olfr73) 10 ug
$750.00

Plasmid Map for MR215298

Download sequence for

Cloning scheme for MR215298

Circular map for MR215298

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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