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Fam217a (NM_027967) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR215143
Fam217a (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700026J04 gene (1700026J04Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $475.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fam217a
Synonyms 1700026J04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215143 representing NM_027967
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAGAAAAAGCAATGAGAGCTGCAGCAGCAGCCTGCATGTGTCAAGCATCTCTCAGGAGAATCACT
CTCAGTGGAACCTGGATGCAGAAGAACTCTTTGATGAAAAAGAAACCTTCTCATCTGAAAATGATGGGGC
AGCAGGTGGCAAAACTAACAAGAGCCATCTGGAAAATCCGGCAGAACAGCTGATATTGCAGCTGACCGTA
TCAGAACATGCTCACAGTAGATCACAGAACAGTAGTCAAGGGGTATTTCAGCTATGGAGTTCTCCTGTTA
ATAAAGGAAGTACCGTAGATAAAAGAGAATTCAAAAACTCTTCAGTGGAAACTGGCTTCAATGCAGCCAA
CAGTCCCAGGCTCTTCACTGTAAACCACTTATTAGCAAGTATTCCGGGTTTGGCAGATAGCAAGCAAGTC
ATTCTTTATCCTGGTCAGTCAATGCCAGCAGGTCTGTGCTGGCCTTATGCTGATGGAGACTTTCTTAAGG
ATAGAAATGAGTTTCATATTAACTCGTGTTCAACTATGGAAAACAACAATGGTGACTCTTTATCTGCTTC
AAATTGGAATTTCAAATACAGAAACAGCAGTGTGGAAGAAAATGTCACAGACGAGAGTGACTTGTCCGAA
AACGAGAAAATGAATGACTCTCTGCTGAGCTACTTTAAAAAGATGGACCTGAACTTAAAGCCGGAAACGA
TAGAACACGTGGAGAGACCTTTCCCAGAGGAAGCAGGCCAAGTGCCTGTATATGCCGACTTTCTCCCTGC
CCCTTTCAACACTCTGGACCTACACAGATTTGCCTTCTCCAAATGTGAAAGCTGGAAGGCGGCAGTGGAA
CCTCCGGAAAGTTCCATTGAGCGTCTGATACTTCGTTTGTTGGAACTGGAAAGATTACAGCATATGACCA
TACAGAGAGAGAGGCCAAGGCTGCAAAGCACTTTCTATAGCTCCATGTTTTCAATGGCAGAACGACCCTC
TTCCTCTAAAGCTATCGCAACGAAAGCCAAGGCGCCAAAGATTCCTGAAACATCGACTCTCCAGACCTCT
GGCGTAGATAAAAACCGAGACAAGAGAAAAAACAATTCTGGCTCTGGCAAGCCAGAACAAAATGTTTCCA
AATGGAGCTTGAGCAGTGCTGGCAAAAGTAAGTCGAACTCTAGAGCACTGCTAAAATGTTCATCCACTTC
AAAGCAGTGTGCTGTGGCTCATGATGACCTAAAGAACTCCAAGAACTCCAGTTTAAACCCTTGCCAGGAA
CCCCCACTCAAGCCTACTACTACTACCCAAGCAACTCAACCAATGGCTAGAGTGGTCTCAAGATGCCTCC
CACCGAGGTCCCCAATGCCAGTTTCCCCTATACCCCTGTCCTTCCCTGAAAATCCAAGGGAAGAAGGTAA
AGTGCCAAGGACCAAAAAGAAATGTCACCGAAAAAGCATACTACTGAATAGAGCTTTCTATATTCAGAAG
CGAAACTGCTTATCACCTTCACTTATAGCCAGGGGCAAGTGCTCACCCACTGACCAAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215143 representing NM_027967
Red=Cloning site Green=Tags(s)

MGRKSNESCSSSLHVSSISQENHSQWNLDAEELFDEKETFSSENDGAAGGKTNKSHLENPAEQLILQLTV
SEHAHSRSQNSSQGVFQLWSSPVNKGSTVDKREFKNSSVETGFNAANSPRLFTVNHLLASIPGLADSKQV
ILYPGQSMPAGLCWPYADGDFLKDRNEFHINSCSTMENNNGDSLSASNWNFKYRNSSVEENVTDESDLSE
NEKMNDSLLSYFKKMDLNLKPETIEHVERPFPEEAGQVPVYADFLPAPFNTLDLHRFAFSKCESWKAAVE
PPESSIERLILRLLELERLQHMTIQRERPRLQSTFYSSMFSMAERPSSSKAIATKAKAPKIPETSTLQTS
GVDKNRDKRKNNSGSGKPEQNVSKWSLSSAGKSKSNSRALLKCSSTSKQCAVAHDDLKNSKNSSLNPCQE
PPLKPTTTTQATQPMARVVSRCLPPRSPMPVSPIPLSFPENPREEGKVPRTKKKCHRKSILLNRAFYIQK
RNCLSPSLIARGKCSPTDQK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027967
ORF Size 1530 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027967.1, NP_082243.1
RefSeq Size 2395 bp
RefSeq ORF 1533 bp
Locus ID 71864
UniProt ID Q9D9W6
Cytogenetics 13 A3.3
MW 56.8 kDa
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Documents
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"NM_027967" in other vectors (4)
SKU Description Size Price
MC217178 Fam217a (untagged) - Mouse RIKEN cDNA 1700026J04 gene (1700026J04Rik), (10ug) 10 ug
$523.00
MG215143 Fam217a (tGFP-tagged) - Mouse RIKEN cDNA 1700026J04 gene (1700026J04Rik), (10ug) 10 ug
$675.00
MR215143L3 Lenti ORF clone of Fam217a (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700026J04 gene (1700026J04Rik) 10 ug
$775.00
MR215143L4 Lenti ORF clone of Fam217a (mGFP-tagged) - Mouse RIKEN cDNA 1700026J04 gene (1700026J04Rik) 10 ug
$775.00

Plasmid Map for MR215143

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR215143
Circular map for MR215143

file-alt Citations

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