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Slfn4 (NM_011410) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR215003
Slfn4 (Myc-DDK-tagged) - Mouse schlafen 4 (Slfn4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $560.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slfn4
Synonyms AI450778
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215003 representing NM_011410
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGGAAACACCCCACAGCTGGAGAAAGATGTGAATCCACAGACATCCATAGAGCCCTCTGTTCAAG
TCAAGTGTCCAGGGGATATTCTTCAGGGAGGACGGCATGCCAAGCGAGGAAGATGGATGGCGTCGTGGAA
AGGATTTCATCTGGGAAGGAGAGACAGTGACTGCAAAATGAACATCACTGTCGATCAGGACACGGACTAC
GCTGAACTGGTTCTGTCTGTAGGAGAAATCACACTCGGAGAGAAGACTAGGGATTCAATGAAAGATAGCC
AACTGAGAAGAAAAGAAGCCAAAAGTATATTACAGGCTGTGTGCACCCTGCTGAATTCTGGAGGGGGTGT
GGTCAAGGCTCACATTAAAAATCAAAACTACAGCTTCACCAGAGATGGAATGGGACTGGATTTGGTAAAT
CCCTTGCCTGGTATCCTGCACCTTCCTCATGACTATCTAGACTTCATGCAGTACAACGACTACTTTTTCG
TTTTTGTGAAACCATGGAAGCCGAATCAGAGAGGTCCGGGGATCGCCACCTGGAAAACCAACTTGTACAA
GAGAATCTTCTCATTCTCAGTTGAACTGAAAGCAGCGGATGCAGTGCAGTTCCTCAAATCCAGACCAAGT
TCCCATGGAAAAACAGTCTGTAATGAAACACTAAATGAATGTCTTTCCTTATTTAACAGAGACTGGCTTG
CCTATGAGGAGACATTCTGTTTCACTAAATCCACACATGCTGAAGTAAAATTGACTCCTAAGGAAAAGAT
TTCTCCTAAGGAAAAGATTTTAGAGCTCCTCCCTCAAACTGTTTCTGCATTTGCAAACACTGATGGGGGA
TATTTGTTCATTGGCTTGGATGGCAAAACGCAGCAAATTATTGGTTTTGAAGCAGAGAAGAGCGATCTCG
TGCTTCTAGAGAGTGAAATAGAAAAGTGCATCCAACAGCTGCCTGTCACTCACTTCTGTGAGGAGAAGGA
GAAGATCAAGTACACGTGCAAATTCATCGAAGTGCACAAATCCGGAGCTGTGTGTGCATATGTGTGCGCG
ATCAGAGTGGAGAAGTTCTGCTGTGCAGTGTTCGCTGCAGAGCCTGAGTCCTGGCACGTGGAAGGCAGCT
GTGTGAAGAGGTTTACCACAGAGGAATGGGTGAAGCGCCAGATGGATGCCACTGCAGTGATGCCTGGAAA
GGTGATATGCTCTCCAGAAGCCCTCTGCATGAAACCATTCTCACAACATGAAGGCTATGAGCAGTTAGTC
CGGACAGAATTGGGCTCCCTGCGTAAAGGAACACTGGTTGTCTCCAAGAGCTGGGCTTTGGATCTGGGCT
TGCAAGAGAAGCAGGAAGTTATCTGGGATGTGCTTCATATTTCCCAGGGCAGTCTCCTGACCCTGCATGT
CTTTGTCCAGGGAGATGAGAACCTGGAAGGCAACAGCAGTCTTCTTGGGAAACTAGGCGCCGAGTTAAAG
GGCTATTATAAACAAATTGCCCTAACATTAAAGCAGACGTTGGTAAACCATTGTGGTTACACTGCGAAAA
TAGGTATTATAGTAAAGATAACATACTTGGGTCATAAGACAATGTGTCTTTATGACTCAAGCGCGAAAAT
ATGTTACCCCCAAATATATTATCTGACTACCAAGGCAGTAAAGGACTTAGAAAAAGCTCTCGCTGAAATC
TTAGGAAGTTATGAGTCTTTCTACAGTTTACCCAGGAGAAACTGGGATTCTTTTATGTCTGCGTTTTTAA
ATGTGGGCAGTTATATAGTTTACTTTTCGAGAAATGTCTTAACTCATATTCGAATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215003 representing NM_011410
Red=Cloning site Green=Tags(s)

MAGNTPQLEKDVNPQTSIEPSVQVKCPGDILQGGRHAKRGRWMASWKGFHLGRRDSDCKMNITVDQDTDY
AELVLSVGEITLGEKTRDSMKDSQLRRKEAKSILQAVCTLLNSGGGVVKAHIKNQNYSFTRDGMGLDLVN
PLPGILHLPHDYLDFMQYNDYFFVFVKPWKPNQRGPGIATWKTNLYKRIFSFSVELKAADAVQFLKSRPS
SHGKTVCNETLNECLSLFNRDWLAYEETFCFTKSTHAEVKLTPKEKISPKEKILELLPQTVSAFANTDGG
YLFIGLDGKTQQIIGFEAEKSDLVLLESEIEKCIQQLPVTHFCEEKEKIKYTCKFIEVHKSGAVCAYVCA
IRVEKFCCAVFAAEPESWHVEGSCVKRFTTEEWVKRQMDATAVMPGKVICSPEALCMKPFSQHEGYEQLV
RTELGSLRKGTLVVSKSWALDLGLQEKQEVIWDVLHISQGSLLTLHVFVQGDENLEGNSSLLGKLGAELK
GYYKQIALTLKQTLVNHCGYTAKIGIIVKITYLGHKTMCLYDSSAKICYPQIYYLTTKAVKDLEKALAEI
LGSYESFYSLPRRNWDSFMSAFLNVGSYIVYFSRNVLTHIRI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011410
ORF Size 1806 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011410.3, NP_035540.2
RefSeq Size 3972 bp
RefSeq ORF 1809 bp
Locus ID 20558
Cytogenetics 11 C
MW 68 kDa
Summary The protein encoded by this gene belongs to the Schlafen family. All members of this family contain a Schlafen box domain that lies near an AAA domain. This protein belongs to the group 2 subset of Schlafen proteins, which are defined by a molecular weight between 58 kDa and 68 kDa and by the presence of a SWADL domain that contains the sequence Ser-Trp-Ala-Asp-Leu. In malignant melanoma cells, gene expression is up-regulated in response to interferon alpha. In bone marrow-derived macrophages, expression of this gene is induced during activation by Toll-like receptor agonists and repressed during macrophage colony-stimulating factor-mediated differentiation. Myelopoiesis is disrupted by constitutive overexpression in myeloid-lineage cells. A pseudogene of this gene is found on chromosome 11. Alternative splicing results in multiple transcript variants. provided by RefSeq, Oct 2014
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"NM_011410" in other vectors (4)
SKU Description Size Price
MC219517 Slfn4 (untagged) - Mouse schlafen 4 (Slfn4), (10ug) 10 ug
$617.00
MG215003 Slfn4 (tGFP-tagged) - Mouse schlafen 4 (Slfn4), (10ug) 10 ug
$760.00
MR215003L3 Lenti ORF clone of Slfn4 (Myc-DDK-tagged) - Mouse schlafen 4 (Slfn4) 10 ug
$860.00
MR215003L4 Lenti ORF clone of Slfn4 (mGFP-tagged) - Mouse schlafen 4 (Slfn4) 10 ug
$860.00

Plasmid Map for MR215003

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR215003
Circular map for MR215003

file-alt Citations

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