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Htra4 (NM_001081187) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214622
Htra4 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 4 (Htra4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Htra4 or NM_001081187"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Htra4
Synonyms B430206E18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214622 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTTCCAGCGGTTGTGGGCTGTGCGAACACAGTTTCTCCTGCTGTGGTTGCTGCTACCAGCCGTGC
CTGTTCCGTGGGCCGAGGCCAGGAGGTCCAGAGTCTCGCTGCCCTGTCCCGATGCCTGCGATCCCACGCG
CTGCCCGACTCTGCCTACCTGCTCGGCCGGCTTGGCGCCGGTTCCCGATCGCTGCGGCTGCTGCCGAGTG
TGCGCCGCGGCCGAGGGCCAGGAGTGTGGCGGGGCGCGGGGCCGGCCGTGCGCCCCAAGGCTGCGCTGCG
GCGCCCCGTTCTCCCGGGATCCCTCGGGGGGCGCGTGGCTGGGCACCTGCGGCTGCGCGGAGGGTGCTGA
GGATGCGGTAGTGTGCGGCAGCGATGGGCGCACCTACCCCAGCCTATGTGCGCTCCGCAAGGAGAACCGA
GCCGCTCGCCAGCGGGGCGCGCTCCCGGCTGTGCCAGTGCAGAAGGGTGCCTGCGAGGAAGCAGGGACCA
CAAGAGCAGGGCGGCTCCGGAGAAAGTACAACTTCATCGCCGCAGTGGTGGAGAAGGTGGCGCCGTCTGT
GGTCCACTTGCAGCTGTTCCGCAGATCACCTCTCACCAACCAGGAAATCCCTTCCTCCAGCGGCTCTGGG
TTCATAGTGTCTGAGGATGGGCTCATTGTCACCAATGCCCACGTCCTCACCAACCAGCAGAAGATCCAGG
TAGAGCTCCAGAGCGGGGCCCGGTATGAAGCCACCGTCAAAGACATCGACCATAAACTGGACCTTGCACT
GATTAAGATCGAGCCAGATACTGAGCTTCCAGTACTGCTGCTGGGCCGATCCTCTGACCTCCGGGCTGGA
GAGTTTGTGGTAGCTTTGGGCAGCCCATTTTCTCTGCAGAACACCGTGACTGCAGGGATTGTCAGCACCA
CACAGAGAGGCGGCAGAGAGCTGGGACTGAAGAATTCAGACATAGACTATATCCAGACGGATGCCATCAT
TAACCATGGAAATTCTGGGGGTCCGCTGGTGAACTTGGATGGCGACGTGATTGGTATAAACACTCTGAAG
GTGACTGCAGGGATCTCTTTTGCGATCCCCTCTGATCGGATCAGACAGTTCCTGGAAGACTATCATGAGC
GCCAGTTGAAAGGCAAGGCCCCTTTGCAGAAGAAATACCTGGGTCTTCGAATGCTGCCTCTCACTCTGAA
CCTCCTTCAGGAAATGAAGAGGCAAGATCCAGAGTTCCCTGATGTGAGTTCTGGAGTTTTTGTATATGAA
GTGATTCAGGGATCGGCTGCTGCAAGCTCGGGGTTGAGAGACCATGATGTAATTGTCAGCATAAACGGGC
AACCTGTCACCACCACAACTGATGTCATTGAAGCTGTTAAGGACAATGACTTTCTCTCCATCATTGTGCT
TCGAGGAAGTCAAACCTTGTTTCTGACAGTCACACCTGAAATAATCAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214622 protein sequence
Red=Cloning site Green=Tags(s)

MSFQRLWAVRTQFLLLWLLLPAVPVPWAEARRSRVSLPCPDACDPTRCPTLPTCSAGLAPVPDRCGCCRV
CAAAEGQECGGARGRPCAPRLRCGAPFSRDPSGGAWLGTCGCAEGAEDAVVCGSDGRTYPSLCALRKENR
AARQRGALPAVPVQKGACEEAGTTRAGRLRRKYNFIAAVVEKVAPSVVHLQLFRRSPLTNQEIPSSSGSG
FIVSEDGLIVTNAHVLTNQQKIQVELQSGARYEATVKDIDHKLDLALIKIEPDTELPVLLLGRSSDLRAG
EFVVALGSPFSLQNTVTAGIVSTTQRGGRELGLKNSDIDYIQTDAIINHGNSGGPLVNLDGDVIGINTLK
VTAGISFAIPSDRIRQFLEDYHERQLKGKAPLQKKYLGLRMLPLTLNLLQEMKRQDPEFPDVSSGVFVYE
VIQGSAAASSGLRDHDVIVSINGQPVTTTTDVIEAVKDNDFLSIIVLRGSQTLFLTVTPEIIN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081187
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081187.3, NP_001074656.1
RefSeq Size 2181 bp
RefSeq ORF 1452 bp
Locus ID 330723
UniProt ID A2RT60
Cytogenetics 8 A2
MW 52 kDa
Summary Serine protease.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081187" in other vectors (4)
SKU Description Size Price
MC216746 Htra4 (untagged) - Mouse HtrA serine peptidase 4 (Htra4), (10ug) 10 ug
$503.00
MG214622 Htra4 (tGFP-tagged) - Mouse HtrA serine peptidase 4 (Htra4), (10ug) 10 ug
$657.00
MR214622L3 Lenti ORF clone of Htra4 (Myc-DDK-tagged) - Mouse HtrA serine peptidase 4 (Htra4) 10 ug
$757.00
MR214622L4 Lenti ORF clone of Htra4 (mGFP-tagged) - Mouse HtrA serine peptidase 4 (Htra4) 10 ug
$757.00

Plasmid Map for MR214622

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214622
Circular map for MR214622

file-alt Citations

HtrA4 is well conserved only in higher primates and functionally important for EVT differentiation
Wang, Li, Nie
Placenta (2024) 152, 53-64 DOI: 10.1016/j.placenta.2024.05.132
Cited by 8
AI Summary: OriGene products—specifically the human HtrA4 FL-ORF construct (RC209174), mouse HtrA4 FL-ORF construct (MR214622), and empty control vector (PS100001)—were used to transfect cells and study the functional role of HtrA4 in trophoblast differentiation in this research.
Snippet: Prior to transfection, cells were cultured overnight in 6-well plates (2.5 × 105/well, then transfected with constructs (5 μg) carrying the full-length open reading frame (FL-ORF) of the human or mouse HtrA4 gene or an empty control vector (RC209174, MR214622, PS100001 respectively, OriGene Technologies, MD, USA) with Lipofectamine 2000 (Thermo Fisher Scientific) for 4 h as per manufacturer's protocol. …
Showing 1-1 of 1 papers.
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