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Exoc6b (NM_177077) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214323
Exoc6b (Myc-DDK-tagged) - Mouse exocyst complex component 6B (Exoc6b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $743.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Exoc6b
Synonyms 4930569O18Rik; G430127E12Rik; mKIAA0919; Sec15b; Sec15l2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214323 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGGGCGAAGATGGCGGAGGAGAGCCTGGAGACGGCGGCGGAGCACGAGCGGATCCTGAGGGAGA
TCGAGAGCACAGACACGGCCTGCATCGGGCCCACGCTCAGGTCTGTCTATGATGGTGAGGAACATGGACG
ATTCATGGAGAAGCTAGAAACTCGTATCCGCAATCATGACAGAGAAATCGAGAAAATGTGCAACTTTCAC
TACCAGGGCTTTGTGGACTCTATTACTGAGCTGCTGAAAGTACGAGGAGAAGCCCAGAAACTCAAAAACC
AGGTGACGGACACTAATAGGAAACTGCAGCACGAGGGCAAGGAACTGGTAATAGCAATGGAGGAGCTGAA
GCAGTGTCGACTACAACAGAGAAATATTTCTGCCACTGTCGATAAGCTAATGCTGTGTCTTCCAGTCCTA
GAGATGTACAGCAAACTGAGGGACCAGATGAAAACCAAAAGGCATTATCCTGCACTGAAGACACTGGAGC
ATCTGGAACACACCTATCTGCCTCAAGTGAGCCACTATCGATTCTGTAAGGTGATGGTGGACAACATCCC
CAAGCTTCGGGAGGAAATAAAAGATGTTTCTATGTCTGATCTCAAAGACTTTCTGGAGAGCATCCGCAAG
CATTCAGACAAAATTGGAGAGACTGCCATGAAGCAAGCCCAACAGCAAAGAAACCTGGATAACATTGTCT
TGCAACAGCCTAGAATAGGTAGCAAAAGGAAATCTAAGAAAGATGTTTATACAATCTTTGATGCAGAGGT
GGAGAGCACAAGTCCAAAGTCGGAACAGGATTCGGGAATTCTGGATGTGGAAGACGAGGAAGATGATGAA
GAGGTACCTGGGGCTCAAGACTTGGTGGATTTCTCTCCTGTGTATCGGTGTCTACACATATATTCTGTCC
TGGGTGCCCGTGAAACATTTGAGAATTACTACCGAAAACAGAGGCGAAAACAGGCTCGTTTGGTACTCCA
GCCTCCGTCTAATATGCATGAAACTTTAGATGGCTACAGGAAGTATTTCAATCAAATTGTAGGCTTTTTT
GTGGTTGAAGATCACATTTTGCATACAACCCAGGGCTTAGTGAATAGAGCCTACATTGATGAACTGTGGG
AAATGGCCCTTTCAAAGACCATCGCAGCATTGCGTACCCACTCGTCCTACTGTTCTGATCCAAACCTCGT
GTTAGATTTGAAGAACCTCATTGTACTCTTTGCGGACACACTTCAGGTGTATGGTTTTCCTGTGAATCAG
CTTTTTGATATGTTGTTAGAAATCAGAGACCAGTACAGCGAGACTCTATTGAAGAAGTGGGCAGGGGTTT
TCAGAAATATACTTGATTCTGACAACTACAGTCCCATACCAGTAACAAGTGAAGAGACATATAAAAAAGT
CGTAGGACAATTCCCGTTTCAAGATATAGAACTGGAAAAGCAGCCATTTCCCAAAAAGTTTCCTTTCTCT
GAATTTGTGCCAAAAGTTTACAACCAGATTAAAGAATTTATCTACGCCTGCCTGAAGTTTTCAGAAGATC
TTCATCTAAGCTCCACTGAAGTCGATGACATGATCCGTAAGTCTACAAACTTGTTGCTGACCAGGACTCT
GAGCAACTCTCTGCAGAACGTCATTAAGAGGAAGAACATTGGCCTTACTGAGCTTGTCCAGATTATCATC
AATACAACACACTTGGAGAAGTCCTGTAAATACCTGGAGGAATTCATCACCAATATCACTAATGTCCTTC
CAGAGACGGTCCATACCACCAAGCTTTATGGTACCACAACTTTCAAGGATGCTAGACATGCAGCTGAAGA
AGAGATATATACAAACCTAAATCAGAAGATTGACCAGTTTCTGCAGCTGGCTGACTACGACTGGATGACT
GGAGACCTAGACAACAAAGCCAGTGATTACCTGGTAGACCTCATTGCCTTCCTCCGGAGCACCTTTGCTG
TGTTCACACACCTTCCTGGAAAGGTGGCTCAGACAGCATGTATGTCAGCTTGCAAGCATTTAGCCACATC
CTTGATGCAACTTTTGCTAGAAGCGGAAGTACGGCAGCTCACTTTGGGAGCATTGCAGCAGTTCAACCTG
GATGTCAGAGAATGTGAACAGTTTGCCAGATCCGGCCCGGTGCCTGGGTTCCAGGAGGACACGCTGCAGT
TGGCCTTCATCGACTTGAGACAACTATTGGACCTCTTTATTCAGTGGGATTGGTCCACATACCTTGCCGA
CTATGGACAGCCCAACTGCAAGTATCTCCGGGTGAACCCTGTGACAGCTCTGACCCTGCTTGAGAAGATG
AAAGATACTAGCCGCAAGAATAACATGTTTGCACAGTTCCGGAAAAATGAGCGAGATAAGCAGAAATTGA
TTGACACCGTGGCCAAGCAGCTCCGAGGACTCATCAGCAGCCACCACTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214323 protein sequence
Red=Cloning site Green=Tags(s)

MERAKMAEESLETAAEHERILREIESTDTACIGPTLRSVYDGEEHGRFMEKLETRIRNHDREIEKMCNFH
YQGFVDSITELLKVRGEAQKLKNQVTDTNRKLQHEGKELVIAMEELKQCRLQQRNISATVDKLMLCLPVL
EMYSKLRDQMKTKRHYPALKTLEHLEHTYLPQVSHYRFCKVMVDNIPKLREEIKDVSMSDLKDFLESIRK
HSDKIGETAMKQAQQQRNLDNIVLQQPRIGSKRKSKKDVYTIFDAEVESTSPKSEQDSGILDVEDEEDDE
EVPGAQDLVDFSPVYRCLHIYSVLGARETFENYYRKQRRKQARLVLQPPSNMHETLDGYRKYFNQIVGFF
VVEDHILHTTQGLVNRAYIDELWEMALSKTIAALRTHSSYCSDPNLVLDLKNLIVLFADTLQVYGFPVNQ
LFDMLLEIRDQYSETLLKKWAGVFRNILDSDNYSPIPVTSEETYKKVVGQFPFQDIELEKQPFPKKFPFS
EFVPKVYNQIKEFIYACLKFSEDLHLSSTEVDDMIRKSTNLLLTRTLSNSLQNVIKRKNIGLTELVQIII
NTTHLEKSCKYLEEFITNITNVLPETVHTTKLYGTTTFKDARHAAEEEIYTNLNQKIDQFLQLADYDWMT
GDLDNKASDYLVDLIAFLRSTFAVFTHLPGKVAQTACMSACKHLATSLMQLLLEAEVRQLTLGALQQFNL
DVRECEQFARSGPVPGFQEDTLQLAFIDLRQLLDLFIQWDWSTYLADYGQPNCKYLRVNPVTALTLLEKM
KDTSRKNNMFAQFRKNERDKQKLIDTVAKQLRGLISSHHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177077
ORF Size 2430 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177077.2, NP_796051.2
RefSeq Size 5440 bp
RefSeq ORF 2433 bp
Locus ID 75914
UniProt ID A6H5Z3
Cytogenetics 6 C3
MW 94.1 kDa
Summary Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.UniProtKB/Swiss-Prot Function
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Documents
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"NM_177077" in other vectors (4)
SKU Description Size Price
MC221767 Exoc6b (untagged) - Mouse exocyst complex component 6B (Exoc6b), (10ug) 10 ug
$817.00
MG214323 Exoc6b (tGFP-tagged) - Mouse exocyst complex component 6B (Exoc6b), (10ug) 10 ug
$943.00
MR214323L3 Lenti ORF clone of Exoc6b (Myc-DDK-tagged) - Mouse exocyst complex component 6B (Exoc6b) 10 ug
$1,043.00
MR214323L4 Lenti ORF clone of Exoc6b (mGFP-tagged) - Mouse exocyst complex component 6B (Exoc6b) 10 ug
$1,043.00

Plasmid Map for MR214323

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214323
Circular map for MR214323

file-alt Citations

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