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E230025N22Rik (NM_172831) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214152
E230025N22Rik (Myc-DDK-tagged) - Mouse Riken cDNA E230025N22 gene (E230025N22Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "E230025N22Rik or NM_172831"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol E230025N22Rik
Synonyms EG240216
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214152 representing NM_172831
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCCTCTGTGGGACAAGGGTACAATACAACCCTCCTGCTCCAGGGCCAGGAAACAGAGGCACCCA
GGTTTGTGCCACAGGTCCTGCAGATGCTGTTTGAAGAAGCTCTGCCTCTCTGCAGCTCTGGCCCTGTGCT
TTGCACTCTCAGTCTGGTGCAGATTAACCGTATTGGACAGGCTCGGGACCTGCTTTCCCCATGTGTGGAG
GACCTGCCAGTACTGGATGTGGCCCCTCTGGGCTTGGTGGTGAAAGATGCCAGTGAAGTGGAAGTGTCTG
ATGCCCGAGCTGCCTCTGAGCTGTACCTAAAGGCTGCTGTGGGTGAAGGCAGAGACTGCCCCCTGCTGCG
AGTATTGGCTGGTGCAGCTGCTGGTGAGGAGGTGGAGGGCTCTCTGCCTTGGATCATCTCCTGGCTCCTG
GAAGCAAACAGCTACAGAGGTGTACTTCTTCGTCTGGACCCCCGAGGTAGCTCCCTGAGTTTGCTCCAGA
ATGCTTTGTTGGGGGCCTCAAGAAAGAGGATGCAGGTGAATGATGTGAAGCCCACCCTATGGAGTGCAGT
AGAGGAGATGGGGGCCCGCAGGGCCACTCTGAAGATGCTGCGCTTAGGCCTTCTTGGAGACACCTTGACA
TACAGTGGGTTGAACCAACTAGGAAGGGCACTCTGGGAACTACAGGTTGTAAAAGCCTGGGGCCCAAGAA
GCCACACCCACAAAGGGACAAGGGATGAGACTGTGAGGCTCCTTGAACCCCAGGTTAAAGGCGAGCCACT
GAACTACTGTAAGCAGGGAAGGCACTCTGCTCATTTGTCAGAGGCAGGAAGAAGAGGATTTCTAGGGTCT
GGGCTCCGCCTAAAGCATCCCCTTGGGCACTGTGAGGAACAGGCCCATCAGGCCCCAGATGTGGCCCTGC
AGTTCTCTCTGGCCCAGGCCCGGAGGCAGAGGCTTCGAGAGGAACACCAGATTAGGATCCAGGAAGAGCT
GAAACATTTGGAACACTGGAAGGAAGTGGCCACATGTGAACAGATCAAAGGCACCGTGGCAGAAGAGGCT
TGCATGGGGAGAGAGAGTCAACGTAAGGAGCAGGCAGTGCTAAAACTTCAGGTGGACACCCTGCAGGCAG
AACGGGACGTGGCAGAGCAAGATCTGGTGGTCCTATATGACTTGTATGTACAGGCCACCCGTGCTCGGAC
GTGCCACTTGCTGCAGGTATTCCAAGCATGGCAGAGGATGTGGGAGGAGAAAGCCATGGCCACAGAGCAT
CACTACCGCAGTCTGCTGGCTGGCATCCTCCAAGGCTCCATTGATCTGGCCTTGAAGAATCAACAACTCC
AGTCCCAAAACCAGCAGCTTGAGCAGAGTGCAGACAGGGCCAGCCATGCTGGAGTCCTGCCTGGAGAGAC
ATCTGATCCAGAACACTCCGGGGCTACCTTCCTTTGTCCTCATTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214152 representing NM_172831
Red=Cloning site Green=Tags(s)

MLASVGQGYNTTLLLQGQETEAPRFVPQVLQMLFEEALPLCSSGPVLCTLSLVQINRIGQARDLLSPCVE
DLPVLDVAPLGLVVKDASEVEVSDARAASELYLKAAVGEGRDCPLLRVLAGAAAGEEVEGSLPWIISWLL
EANSYRGVLLRLDPRGSSLSLLQNALLGASRKRMQVNDVKPTLWSAVEEMGARRATLKMLRLGLLGDTLT
YSGLNQLGRALWELQVVKAWGPRSHTHKGTRDETVRLLEPQVKGEPLNYCKQGRHSAHLSEAGRRGFLGS
GLRLKHPLGHCEEQAHQAPDVALQFSLAQARRQRLREEHQIRIQEELKHLEHWKEVATCEQIKGTVAEEA
CMGRESQRKEQAVLKLQVDTLQAERDVAEQDLVVLYDLYVQATRARTCHLLQVFQAWQRMWEEKAMATEH
HYRSLLAGILQGSIDLALKNQQLQSQNQQLEQSADRASHAGVLPGETSDPEHSGATFLCPHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172831
ORF Size 1446 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172831.2, NP_766419.2
RefSeq Size 2016 bp
RefSeq ORF 1449 bp
Locus ID 240216
Cytogenetics 18 B2
MW 53.4 kDa
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Documents
Resources
"NM_172831" in other vectors (4)
SKU Description Size Price
MC216729 E230025N22Rik (untagged) - Mouse Riken cDNA E230025N22 gene (E230025N22Rik), (10ug) 10 ug
$503.00
MG214152 E230025N22Rik (tGFP-tagged) - Mouse Riken cDNA E230025N22 gene (E230025N22Rik), (10ug) 10 ug
$657.00
MR214152L3 Lenti ORF clone of E230025N22Rik (Myc-DDK-tagged) - Mouse Riken cDNA E230025N22 gene (E230025N22Rik) 10 ug
$757.00
MR214152L4 Lenti ORF clone of E230025N22Rik (mGFP-tagged) - Mouse Riken cDNA E230025N22 gene (E230025N22Rik) 10 ug
$757.00

Plasmid Map for MR214152

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214152
Circular map for MR214152

file-alt Citations

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