Borcs6 (NM_028005) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR214131
2310047M10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310047M10 gene (2310047M10Rik)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Borcs6
Synonyms 2310047M10Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214131 representing NM_028005
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCGCCCAGGGGCGGCTCGGGCCGGAGCCGGAGCTTTCGGTCGGAGCTGAGCACCAGGCCGCGA
CTTTCAGCGGCAGGCCGTCCCGAACGCCCTCGAAGCCGCCCTCAGTCCGGACGCTGTCCGGGGAGGAAGA
GGCTGAGAGCGTTGGGGTCTCGAGCCGTCATCCCCGAGCGTCCCCAAAGACTTGGAGCGGCAGCATCGCC
CATGGGCCGGAGCTGGACACTTGGGAGGACAAGCCGAGCTCCCGGGCGACGCCGTCAGGGGCCCGGGGAC
GCCGGGGGGTGCCAGGTTCCGAGCACGCCCCGCCCCCGTCCTCCTGGTACCCGGAGCCGGAGCCCAGCGA
GGATCAGCCCTCCGCTCTGAGGGTCTGCCGTCGAGGGAGCCCCGGAGGCGTCGAGATGAATGTGGAGCTG
CCGCAGCAAGAGGGTGACGACGACGACGACGAGGACGAAGAGGCGGCGGCGGGTAGGGCTGGCCGCTCCT
TCCCCAGCCGCCTTCAGGACAGCCGCAGCCTGGACGGGCTGAGTGGGGCGTGCGGTGGAGGGGGGTCTTC
ATCCAGTGGCGAGACCGGCGCGGGCGGAGGACGTCGCGCCACTATCTCCAGCCCCCTAGAGCTGGAGGGC
ACGGTGAGCCGCCACGGCGACCTTACTCACTTTGTGGCCAACAACCTACAACTCAAGATCCGACTGAGTG
GCGCCCCACCACCCGTTCCCCCTGCCTCTGTGCGGCCCTGCCTAACGCCCGCACCCACCCCCACCATCCC
TCCCATCGACCCGGACGTGCTGAGGGATCTGGAGCGGCTGAGTCGCGAGCTGGGCGGCCGGGTAGACCGT
CTGCTTCGCGGGCTGGGTGGTGCGGTGCAAGAGCTGACAGCGTTGAGTGTGGGCTGTATCCAGACCTACC
GCGACGCGGTGGACTCCTTAGGTGAAGCCGTGGACATGAGCATTAAGGGCATGTACACCTTACTTGCGCG
CTGCGAGGAGCTGGAACGGGCTTTGCAACCGGTTCAGGGGCTGGCACGCCAAGTCCGAGATATCCGACGC
ACGCTGGAGGTGTTGGAGGCCCTATGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR214131 representing NM_028005
Red=Cloning site Green=Tags(s)

MEAAQGRLGPEPELSVGAEHQAATFSGRPSRTPSKPPSVRTLSGEEEAESVGVSSRHPRASPKTWSGSIA
HGPELDTWEDKPSSRATPSGARGRRGVPGSEHAPPPSSWYPEPEPSEDQPSALRVCRRGSPGGVEMNVEL
PQQEGDDDDDEDEEAAAGRAGRSFPSRLQDSRSLDGLSGACGGGGSSSSGETGAGGGRRATISSPLELEG
TVSRHGDLTHFVANNLQLKIRLSGAPPPVPPASVRPCLTPAPTPTIPPIDPDVLRDLERLSRELGGRVDR
LLRGLGGAVQELTALSVGCIQTYRDAVDSLGEAVDMSIKGMYTLLARCEELERALQPVQGLARQVRDIRR
TLEVLEALCK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028005
ORF Size 1080 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028005.3, NP_082281.2
RefSeq Size 1802 bp
RefSeq ORF 1083 bp
Locus ID 71923
UniProt ID Q9D6W8
Cytogenetics 11 B3
MW 38 kDa
Summary As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028005" in other vectors (4)
SKU Description Size Price
MC211254 2310047M10Rik (untagged) - Mouse RIKEN cDNA 2310047M10 gene (2310047M10Rik), (10ug) 10 ug
$503.00
MG214131 2310047M10Rik (tGFP-tagged) - Mouse RIKEN cDNA 2310047M10 gene (2310047M10Rik), (10ug) 10 ug
$657.00
MR214131L3 Lenti ORF clone of 2310047M10Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2310047M10 gene (2310047M10Rik) 10 ug
$757.00
MR214131L4 Lenti ORF clone of 2310047M10Rik (mGFP-tagged) - Mouse RIKEN cDNA 2310047M10 gene (2310047M10Rik) 10 ug
$757.00

Plasmid Map for MR214131

Download sequence for

Cloning scheme for MR214131

Circular map for MR214131

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