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Tmem134 (NM_025889) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214122
Tmem134 (Myc-DDK-tagged) - Mouse transmembrane protein 134 (Tmem134), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tmem134 or NM_025889"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem134
Synonyms 2410001H17Rik; AI463452
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214122 representing NM_025889
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGCCGCCCGGCCCCAGTTTAGCATCGATGATGCCTTCGAGCTGACCCTGGAGGACGCGGGGCCTG
AGCCCGAGTCCAGCGGGGTCGCCCGCTTCGGGCCGCTGCACTTCGAACGCCGGGCTCGGTTCGAGGTGGC
TGATGAAGACAAGCAGTCCCGGCTACGCTACCAGAACCTGGAAAATGATGAGGACGGAGCCCAGGCCTCT
CCGGAGCCGGATGGGGGAGTCAGCACCAGGGATTCTGGGCACATGTCTGTCCGCAGCTCGCAGTGGTCCT
TCAGCACCATCAGCAGCAGCACACAGCGCTCCTACAACGCCTGCTGCAGCTGGACCCAACATCCTTTGAT
CCAGAAAAACCGCCGGGTAGTGCTGGCCTCCTTCCTGCTTCTGCTGTTGGGGCTAGGTGTCTCCAGTGCC
ATCTTCTTTGTGCCGGGCATCCTGCTGTTAGTCCCAGGAGTCTACCATGTGATCTTCATCTACTGCGCTG
TCAAGGGCCGCCGGGGCTTCCAATTCTTCTACCTGCCCTACTTTGAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214122 representing NM_025889
Red=Cloning site Green=Tags(s)

MSAARPQFSIDDAFELTLEDAGPEPESSGVARFGPLHFERRARFEVADEDKQSRLRYQNLENDEDGAQAS
PEPDGGVSTRDSGHMSVRSSQWSFSTISSSTQRSYNACCSWTQHPLIQKNRRVVLASFLLLLLGLGVSSA
IFFVPGILLLVPGVYHVIFIYCAVKGRRGFQFFYLPYFEK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025889
ORF Size 540 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025889.2, NP_080165.1
RefSeq Size 1472 bp
RefSeq ORF 543 bp
Locus ID 66990
UniProt ID Q8R0J4
Cytogenetics 19 A
MW 20.6 kDa
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"NM_025889" in other vectors (3)
SKU Description Size Price
MG214122 Tmem134 (tGFP-tagged) - Mouse transmembrane protein 134 (Tmem134) transcript variant 2, (10ug) 10 ug
$500.00
MR214122L3 Lenti ORF clone of Tmem134 (Myc-DDK-tagged) - Mouse transmembrane protein 134 (Tmem134), transcript variant 2 10 ug
$600.00
MR214122L4 Lenti ORF clone of Tmem134 (mGFP-tagged) - Mouse transmembrane protein 134 (Tmem134), transcript variant 2 10 ug
$600.00

Plasmid Map for MR214122

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214122
Circular map for MR214122

file-alt Citations

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