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Olfr421-ps1 (NM_146720) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214068
Olfr421 (Myc-DDK-tagged) - Mouse olfactory receptor 421 (Olfr421)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Olfr421-ps1 or NM_146720"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr421-ps1
Synonyms MOR105-3; Olfr421; Olfr422-ps1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214068 representing NM_146720
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAATGGAAACCTATCTACCGTGACTGTGTTTGTCTTCACTGGGTTCCCCCAGCTCAAGAATGGTG
GCCTCCTGTACTTTTTTCCTCTACTGTTCATTTACATCTTTATTGTGACAGGAAACCTGATGATCTTTTT
TGCTGTAAGGTTGGACACTCGTCTTCATAATCCCATGTACAATTTCATAAGTATCTTCTCATTTCTTGAG
ATGTGGTATACAACAGCTACCATACCTAAGATGCTCTCCAATCTGATCAGTGAGCAAAAGACCATTTCCT
TCATTGGCTGCCTCTTGCAGATGTACTTCTTCCATTCACTTGGAAATACCGAAGGGACCCTTCTGACTGT
CATGGCTATTGACAGGTATGTTGCCATCTGTAATCCACTCCGCTACCCAAACATCATGACACCCAGGCTC
TGTGCTCAGCTCACTGCTGGTTCTTGTGTATTTGGCTTCCTCATCCTTCTACCTGAGATTGTGTGGATTT
CTACTCTACCTTTTTGTGGCCCCAATCAAATCACTCAGATATTCTGTGATTTCACCCCTTTGTTGAAGTT
AGCCTGTACAGATGCCTCAGTGATCTTGGTTCAAGATGTGATACATGCTCTCGCTATTCTGATCACAAGT
CTGATTATTTCTCTTTCTTACATAAGAATTATTGTTGTTATCCTGAGCATCTCTTCAACAGAAGGTCGCA
AAAAAGCTTTTTCCACTTGTGCTGCCCACATTGCTGTCTTCCTCTTGTTTTTTGGCAGTGTGGCCCTTAT
GTATCTTCGATTCTCTGCCACCTATACACCATTCTGGGACAACACCATTGCTCTTACATTTTCTGTGTTT
GCCCCTCTTTTCAATCCTATTATATACAGCCTGAGAAATAAGGACATGAAAGATGCAATTAACAAACTCA
TTTGTACTCAAAAAGTATCCAATGAATCACATAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214068 representing NM_146720
Red=Cloning site Green=Tags(s)

MENGNLSTVTVFVFTGFPQLKNGGLLYFFPLLFIYIFIVTGNLMIFFAVRLDTRLHNPMYNFISIFSFLE
MWYTTATIPKMLSNLISEQKTISFIGCLLQMYFFHSLGNTEGTLLTVMAIDRYVAICNPLRYPNIMTPRL
CAQLTAGSCVFGFLILLPEIVWISTLPFCGPNQITQIFCDFTPLLKLACTDASVILVQDVIHALAILITS
LIISLSYIRIIVVILSISSTEGRKKAFSTCAAHIAVFLLFFGSVALMYLRFSATYTPFWDNTIALTFSVF
APLFNPIIYSLRNKDMKDAINKLICTQKVSNESHR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146720
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146720.3, NP_666931.3
RefSeq Size 1050 bp
RefSeq ORF 948 bp
Locus ID 258715
Cytogenetics 1 H3
MW 35.6 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene appears to represent a strain-specific polymorphic pseudogene in mouse, where some strains, including the C57BL/6 reference genome strain, have a non-functional allele, while other strains have a protein-coding allele. provided by RefSeq, Apr 2012
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"NM_146720" in other vectors (4)
SKU Description Size Price
MC213867 Olfr421 (untagged) - Mouse olfactory receptor 421 (Olfr421), (10ug) 10 ug
$330.00
MG214068 Olfr421 (tGFP-tagged) - Mouse olfactory receptor 421 (Olfr421), (10ug) 10 ug
$500.00
MR214068L3 Lenti ORF clone of Olfr421 (Myc-DDK-tagged) - Mouse olfactory receptor 421 (Olfr421) 10 ug
$600.00
MR214068L4 Lenti ORF clone of Olfr421 (mGFP-tagged) - Mouse olfactory receptor 421 (Olfr421) 10 ug
$600.00

Plasmid Map for MR214068

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214068
Circular map for MR214068

file-alt Citations

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