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2610318N02Rik (NM_183287) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR214032
2610318N02Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2610318N02 gene (2610318N02Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 2610318N02Rik
Synonyms AL033325
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR214032 representing NM_183287
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTCCTGGATGAGTTCGACAACGAATTCCCCCTGAGTGTGACCTTCTGCCAGCTCATTTCCGAGG
ATGACTTCGAGAGGCAAGCAGCGACCTACACAGAGCGTGCACTGCGACGACTCTTCCGCACCTTGGATCG
CAACCCAGCACTGGCGGAGAGAGTCGTGCGGAAGGGCAAGCAGACTGAGTTCGAGCAGCGCGGGCTCCTC
TGCAGCCTCTGGGCGAAGTTTGTCTGCGCTGTGCAGGGGGATCTAAACCAGTGCAATGACATGGGTGCCA
TAGAGATGCATCGGCGCCTGGAGCAGCTGAAGAGGCGCATACATCGTGTGCATCTGTATTCCCAGGATGC
CAAGAAGAGGAGGAGGAGGCCAAAACCAAAGAAACCAGATCCTATCTTTCGAGATCAGTCAACCTCCGCA
TGCCTGCCACCAGCTCTCTTACCACCACCGCTGCTGCCGCCACCACCACAACCTGTCACCACCGTGGCCT
CTGTAGTGGCTGCATCACCCTCTGTCTACACTATGCCCCGGGTCTTTGGCCCTTTCCCTCCACTGTCTGG
CAAGCCGATGGAGAAACTGGATATTTCAAGTTCTGAAGTGAAACTAAACTGGAGTGGTCTTTCATCAACG
TCAAAGAGCCACATGGTTGATCTGACCCCCTTGGTTCTCAATCCCGGAGGCTTCCATTTCTCATATTTGG
CAGGAAGCAGTGCACACAAGCTCCATTGTCCTGGCTTTCCCTGGACGCCAGCCTGTAGCCGACCCTCCAA
CACGGATGAGACGCCATCTCCTGTTGTGAAAACCTCCATCTTCACTCCGCCTGTACTGCTCAAGTTCCAA
CCTGTGCCCAGACCCAATGATGATTCAGAGAATGACTCTGGCAGCGCAGAGTCCACTCCTGCCAAGGAGA
GCCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR214032 representing NM_183287
Red=Cloning site Green=Tags(s)

MEVLDEFDNEFPLSVTFCQLISEDDFERQAATYTERALRRLFRTLDRNPALAERVVRKGKQTEFEQRGLL
CSLWAKFVCAVQGDLNQCNDMGAIEMHRRLEQLKRRIHRVHLYSQDAKKRRRRPKPKKPDPIFRDQSTSA
CLPPALLPPPLLPPPPQPVTTVASVVAASPSVYTMPRVFGPFPPLSGKPMEKLDISSSEVKLNWSGLSST
SKSHMVDLTPLVLNPGGFHFSYLAGSSAHKLHCPGFPWTPACSRPSNTDETPSPVVKTSIFTPPVLLKFQ
PVPRPNDDSENDSGSAESTPAKESQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183287
ORF Size 915 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183287.3
RefSeq Size 1357 bp
RefSeq ORF 918 bp
Locus ID 70458
Cytogenetics 16 A3
MW 34 kDa
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Documents
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"NM_183287" in other vectors (4)
SKU Description Size Price
MC211072 2610318N02Rik (untagged) - Mouse RIKEN cDNA 2610318N02 gene (2610318N02Rik), (10ug) 10 ug
$330.00
MG214032 2610318N02Rik (tGFP-tagged) - Mouse RIKEN cDNA 2610318N02 gene (2610318N02Rik), (10ug) 10 ug
$500.00
MR214032L3 Lenti ORF clone of 2610318N02Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2610318N02 gene (2610318N02Rik) 10 ug
$600.00
MR214032L4 Lenti ORF clone of 2610318N02Rik (mGFP-tagged) - Mouse RIKEN cDNA 2610318N02 gene (2610318N02Rik) 10 ug
$600.00

Plasmid Map for MR214032

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR214032
Circular map for MR214032

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