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2210016F16Rik (NM_027335) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR213952
2210016F16Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2210016F16 gene (2210016F16Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 2210016F16Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213952 representing NM_027335
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCCACCGCTTTCGCCGCGCGAGTCGGCGAGGTTCGTGGCCGAGAACAGTCGCGACGTGCTCGTGG
ACCACGAGGGCGTGCGCAGGGCGGCCGAGCTGCTGCTGCCCGCGGCCGCGGCGTGGCGCGTGGAGCAGTG
GAAGTCCCTGCACGAGCTCAACCCGCGCGGGGCGGACGAGGCTGCGCTCGGCTGGGTGTTCCTGGTGGAC
TCGCTCAACTTCTCCTTCTGGGCCGAGCGGGAGGACAGCAAGTGTGCGGTGCGCTACGGCGGGACGCCCT
ACACTGGCTACTGGGCCCTGTGCGCCGCCGTCAACCGCGCGCTCGACCAAGGGATACCGATAACCAGCGC
CTCCTACTACGCCACAGTGAGCCTCGAGCAGGTGCGGGATATATTTCGTTCAGACACAGCTGTGCCCATG
CCTTTAATGGAAGAGAGACATCGGATTCTCAATGAAACTGGGAAAATCCTACTGGAGAAGTTTGGAGGCT
CTTTTCTTAACTGTGTCCAAAAAAGTGGCAGAAGTGCGCAGAAGTTAATGCAGCTGATTGTTGAGAACTT
CCCTTCTTACAGGGACGAGGCAGAGTTTGAGGGGAAAAGGATCGCCTTTTACAAACGAGCACAGATCCTC
GTGGCGGACACGTGGAGTGTGCTGGAGGGCAAGGGAGATGGCTGCTTCGAGGACATCTCCAGCATCACCA
TGTTTGCTGACTACAGGCTGCCTCAGATTCTCGTGTACCTTGGAGCCCTGAAGTACTCAGATGAGCTACT
AAAGAAGCTCCTCAAAGGAGAGATGCTCTTGAATGGAGATAAACAGGAAGTGGAAATTAGAGGTTGCTCC
ATTTGGTGTGTCGAGCTGATCCGGGACCGTCTTCTGGAGCTTCTTGAAAAGGGTGAAAACTCTCCTGTTG
AGATCAACTCTGTTCTTCTGGACTATCACCTGTGGGACTATGCCCGAGAGCACAGGGAAGACATGAAGGG
AGTCCCTTTTCATCGCACACGCTGCATCTACTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR213952 representing NM_027335
Red=Cloning site Green=Tags(s)

MGPPLSPRESARFVAENSRDVLVDHEGVRRAAELLLPAAAAWRVEQWKSLHELNPRGADEAALGWVFLVD
SLNFSFWAEREDSKCAVRYGGTPYTGYWALCAAVNRALDQGIPITSASYYATVSLEQVRDIFRSDTAVPM
PLMEERHRILNETGKILLEKFGGSFLNCVQKSGRSAQKLMQLIVENFPSYRDEAEFEGKRIAFYKRAQIL
VADTWSVLEGKGDGCFEDISSITMFADYRLPQILVYLGALKYSDELLKKLLKGEMLLNGDKQEVEIRGCS
IWCVELIRDRLLELLEKGENSPVEINSVLLDYHLWDYAREHREDMKGVPFHRTRCIYY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027335
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027335.1, NP_081611.1
RefSeq Size 2822 bp
RefSeq ORF 1017 bp
Locus ID 70153
Cytogenetics 13 B1
MW 38.6 kDa
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Documents
Resources
"NM_027335" in other vectors (4)
SKU Description Size Price
MC211045 2210016F16Rik (untagged) - Mouse RIKEN cDNA 2210016F16 gene (2210016F16Rik), (10ug) 10 ug
$503.00
MG213952 2210016F16Rik (tGFP-tagged) - Mouse RIKEN cDNA 2210016F16 gene (2210016F16Rik), (10ug) 10 ug
$657.00
MR213952L3 Lenti ORF clone of 2210016F16Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2210016F16 gene (2210016F16Rik) 10 ug
$757.00
MR213952L4 Lenti ORF clone of 2210016F16Rik (mGFP-tagged) - Mouse RIKEN cDNA 2210016F16 gene (2210016F16Rik) 10 ug
$757.00

Plasmid Map for MR213952

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR213952
Circular map for MR213952

file-alt Citations

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