Olfr552 (NM_147102) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR213877
Olfr552 (Myc-DDK-tagged) - Mouse olfactory receptor 552 (Olfr552)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr552
Synonyms MOR28-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213877 representing NM_147102
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGGTTAATAATATCACCTCAACCCACCCAGTGGCCTTCTTGTTGATGGGTATCCCAGGCCTAGAGC
ACTTACATATTTGGATCTCCATCCCCTTCTGCTCAGCCTATACCTTGGCTGTGCTTGGCAACTGCACCCT
CCTTTTCATCATTCGGGTTGATGCAGCTCTCCATGAGCCCATGTACCTCTTTCTGGCCATGTTGGCAGCA
ATAGATCTGGTCCTTTCCTCGTCAACACTGCCCAAAATGCTCTCCCTGTTCTGGTTCAGGGATCGGGAAA
TCAATTTCCATGCCTGCCTTATCCAGATGTTCTTTCTCCATTCCTTTGCTATCATGGAGTCAGCAATGTT
GTTGGCCATGGCCTTCGATCGTTATGTTGCCATATGCAAACCTCTGCACTATACCACCATCCTGACCAAG
CCCCTCATCATCAAGATTGGCCTGGCTGCTGTGACTCGGGCTGTGACACTAATGACTCCACTTCCTTTTC
TGCTCAGACGTTTCCATTACTGTCGAGGCACAGTGATTGCCCACTGCTACTGTGAACACATGGCTGTGGT
GAGGCTGGCATGTGGGGACACACGCTTCAATAACATCTATGGCATTGCTGTGGCCATGTTTATTGTGGTG
CTGGACCTGCTCTTCGTTATCTTGTCTTACATCTTCATCCTTCGTGCTGTTCTCCAGCTTGCCTCCCAGG
AGGCTCGCTACAAGGCCTTTGGGACATGTGTGTCGCATATAGGTGCCATCTTAGCCTTCTATACACCTGT
AGTCATCTCCTCAGTCATGCATCGTGTGGCTCGTCGGGCTGCTCCTCATGTCCACATACTCCTTGCTAAT
TTCTATCTTCTTTTCCCACCTATGGTCAATCCTATCATCTATGGTGTCAAGACTAAGCAGATTAGAGAGC
GAGTCTTAGGGCTATTCCTCAGAAAGGATTTAAAAGGTGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR213877 representing NM_147102
Red=Cloning site Green=Tags(s)

MLVNNITSTHPVAFLLMGIPGLEHLHIWISIPFCSAYTLAVLGNCTLLFIIRVDAALHEPMYLFLAMLAA
IDLVLSSSTLPKMLSLFWFRDREINFHACLIQMFFLHSFAIMESAMLLAMAFDRYVAICKPLHYTTILTK
PLIIKIGLAAVTRAVTLMTPLPFLLRRFHYCRGTVIAHCYCEHMAVVRLACGDTRFNNIYGIAVAMFIVV
LDLLFVILSYIFILRAVLQLASQEARYKAFGTCVSHIGAILAFYTPVVISSVMHRVARRAAPHVHILLAN
FYLLFPPMVNPIIYGVKTKQIRERVLGLFLRKDLKGE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_147102
ORF Size 951 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_147102.2, NP_667313.2
RefSeq Size 954 bp
RefSeq ORF 954 bp
Locus ID 259106
Cytogenetics 7 E3
MW 36.3 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_147102" in other vectors (4)
SKU Description Size Price
MC214216 Olfr552 (untagged) - Mouse olfactory receptor 552 (Olfr552), (10ug) 10 ug
$330.00
MG213877 Olfr552 (tGFP-tagged) - Mouse olfactory receptor 552 (Olfr552), (10ug) 10 ug
$500.00
MR213877L3 Lenti ORF clone of Olfr552 (Myc-DDK-tagged) - Mouse olfactory receptor 552 (Olfr552) 10 ug
$600.00
MR213877L4 Lenti ORF clone of Olfr552 (mGFP-tagged) - Mouse olfactory receptor 552 (Olfr552) 10 ug
$600.00

Plasmid Map for MR213877

Download sequence for

Cloning scheme for MR213877

Circular map for MR213877

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