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Apol7e (NM_001134802) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR213353
Apol7e (Myc-DDK-tagged) - Mouse apolipoprotein L 7e (Apol7e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Apol7e
Synonyms ENSMUSG00000071716
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213353 representing NM_001134802
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCACCCCAGACAGAGAATGCTTCATTGAGACTGTGGCTGAGTACCTCCTGGATGTGATAAGCACAG
AGGACCTGCAGCTCCTGCTGACAGAAGAGGAAACCTGGAAACACTTTGTGGCAGAAGTTGATTTGTCCAG
GGAAGAGGAAACTGCCTTGCGAGAAGCTCTTGCAGAGATCTTTTCAGATCCTGATGGAGAAGATGAAGAT
GAGCTCCAAAATGACCTGCAGGACAAGAACGAGAGGAAAGAGGAAGATGCCTTGAGTGAAGCCCTGGGTA
AGACCGTAGCTGATACTGATGCAGAAGATGAAGAAGAGATCCAAAATGACCCGTGGCACAAGGAACGGTT
CTTGGATGCTTATCCTCGGGTGAAACTGGAGCTTGAGGAGAGCATCAGGATGCTCCACGCCCTGGCAGAC
AAGGTTGACAAGGTGCACAGGGACTGCACCATCTCACGGGTGGTGGCCAGCTCCAGCAGTGCTGTGTCTG
GAGTCCTGACCATCCTTGGTCTGTCTCTGGCACCTGTGACAGCAGGAGTCAGTCTGGCATTGTCAGCCAC
TGGCTTGGGGCTGGGGGCAGCAGCGGCTGTGACTAGTGTTTCCACAAGCATTGTGGAAAAGGTAAGTGTG
GTGTCTGCTGAAGCTAAAGCCAGCAAGTTGGTACCAACCAACAAAGACACAATGAAGGGCATGAAAGAAG
TTTTGGATCAGAGTGGTCCCAGACTTCTTTTCTTATCCATGAATTCCTTCAAGAAACTGAAAAACATCAA
GAATAATATTCATGCCATCAAACTGACCAAAGCCAACCCTAGCCTAGTAACTAATGCTAAGCGCCTCATG
ACCACAGGAAAGACAACAACAGAAACCACTAAACAAGTGAAGGAAGCCTTTGGAGGTACTGCTCTGGCAA
TGACCAAAGGAGCCCGGATCATGGGTGCAGCCACCACAGGTTTCTTCCTCCTGCTGGATATAGTTAGCTT
TGTAGGAGACTCAAAGCATTTGCATGAAGGAGCAAAGGCTGAGTCTGCTGCAGAGCTGCGGCATCAGGCT
CAGGACCCAGAACAGAAGTTACAGGAGCTCATTCGGGTCCATGACAGCCTGATTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR213353 representing NM_001134802
Red=Cloning site Green=Tags(s)

MGTPDRECFIETVAEYLLDVISTEDLQLLLTEEETWKHFVAEVDLSREEETALREALAEIFSDPDGEDED
ELQNDLQDKNERKEEDALSEALGKTVADTDAEDEEEIQNDPWHKERFLDAYPRVKLELEESIRMLHALAD
KVDKVHRDCTISRVVASSSSAVSGVLTILGLSLAPVTAGVSLALSATGLGLGAAAAVTSVSTSIVEKVSV
VSAEAKASKLVPTNKDTMKGMKEVLDQSGPRLLFLSMNSFKKLKNIKNNIHAIKLTKANPSLVTNAKRLM
TTGKTTTETTKQVKEAFGGTALAMTKGARIMGAATTGFFLLLDIVSFVGDSKHLHEGAKAESAAELRHQA
QDPEQKLQELIRVHDSLIQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001134802
ORF Size 1107 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001134802.1, NP_001128274.1
RefSeq Size 2227 bp
RefSeq ORF 1110 bp
Locus ID 666348
Cytogenetics 15 E1
MW 40.2 kDa
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"NM_001134802" in other vectors (4)
SKU Description Size Price
MC215376 Apol7e (untagged) - Mouse apolipoprotein L 7e (Apol7e), (10ug) 10 ug
$503.00
MG213353 Apol7e (tGFP-tagged) - Mouse apolipoprotein L 7e (Apol7e), (10ug) 10 ug
$657.00
MR213353L3 Lenti ORF clone of Apol7e (Myc-DDK-tagged) - Mouse apolipoprotein L 7e (Apol7e) 10 ug
$757.00
MR213353L4 Lenti ORF clone of Apol7e (mGFP-tagged) - Mouse apolipoprotein L 7e (Apol7e) 10 ug
$757.00

Plasmid Map for MR213353

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR213353
Circular map for MR213353

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