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Tmem147 (NM_027215) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR213260
Tmem147 (Myc-DDK-tagged) - Mouse transmembrane protein 147 (Tmem147)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tmem147 or NM_027215"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem147
Synonyms 2010004E11Rik; 5033425B17Rik; Nifie14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213260 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCTGTTCCACTTCGGGAACTGCTTCGCCCTCGCCTACTTTCCCTATTTCATCACGTACAAATGCA
GCGGCCTGTCTGAATACAACGCCTTCTGGAAATGCGTCCAGGCCGGGGTCACCTACCTCTTTGTGCAGCT
ATGCAAGATGTTGTTCTTGGCCACTTTCTTCCCCACCTGGGAAGGTGGCATCTATGACTTCATTGGGGAA
TTCATGAAGGCCAGTGTGGATGTGGCAGACCTGATAGGCCTAAACCTTGTCATGTCTCGGAATGCAGGCA
AGGGGGAGTACAAGATTATGGTTGCTGCCCTGGGCTGGGCCACTGCTGAACTCATTATGTCCCGATGCAT
CCCCCTCTGGGTGGGAGCCCGAGGCATTGAATTTGACTGGAAATATATCCAGATGAGCATTGACTCCAAC
ATCAGTCTGGTCCACTACATCGTTGCATCAGCTCAGGTCTGGATGATAACACGCTATGACCTGTACCACA
CCTTCCGGCCTGCTGTTCTCCTCCTGATGTTCCTTAGTGTCTACAAGGCCTTTGTCATGGAGACCTTCGT
CCACCTCTTCTCCTTGGGTAGTTGGACAGCACTGCTCGCCCGGGCAGTAGTAACAGGACTGCTGGCCCTC
AGCACCTTGGCCCTGTATGTTGCTGTTGTCAATGTACACTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR213260 protein sequence
Red=Cloning site Green=Tags(s)

MTLFHFGNCFALAYFPYFITYKCSGLSEYNAFWKCVQAGVTYLFVQLCKMLFLATFFPTWEGGIYDFIGE
FMKASVDVADLIGLNLVMSRNAGKGEYKIMVAALGWATAELIMSRCIPLWVGARGIEFDWKYIQMSIDSN
ISLVHYIVASAQVWMITRYDLYHTFRPAVLLLMFLSVYKAFVMETFVHLFSLGSWTALLARAVVTGLLAL
STLALYVAVVNVHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027215
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027215.2, NP_081491.2
RefSeq Size 868 bp
RefSeq ORF 675 bp
Locus ID 69804
UniProt ID Q9CQG6
Cytogenetics 7 B1
MW 25.3 kDa
Summary Acts as a negative regulator of CHRM3 function, most likely by interfering with its trafficking to the cell membrane (By similarity). Negatively regulates CHRM3-mediated calcium mobilization and activation of RPS6KA1/p90RSK activity (By similarity).UniProtKB/Swiss-Prot Function
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"NM_027215" in other vectors (4)
SKU Description Size Price
MC211004 Tmem147 (untagged) - Mouse transmembrane protein 147 (Tmem147), (10ug) 10 ug
$330.00
MG213260 Tmem147 (tGFP-tagged) - Mouse transmembrane protein 147 (Tmem147), (10ug) 10 ug
$500.00
MR213260L3 Lenti ORF clone of Tmem147 (Myc-DDK-tagged) - Mouse transmembrane protein 147 (Tmem147) 10 ug
$600.00
MR213260L4 Lenti ORF clone of Tmem147 (mGFP-tagged) - Mouse transmembrane protein 147 (Tmem147) 10 ug
$600.00

Plasmid Map for MR213260

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR213260
Circular map for MR213260

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