Olfr704 (NM_001011749) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR213252
Olfr704 (Myc-DDK-tagged) - Mouse olfactory receptor 704 (Olfr704)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr704
Synonyms MOR283-12P
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213252 representing NM_001011749
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTCTGTAACTCCACCTTGAGAAGTGGCTTCATCTTGATGGGTATTTTGGATGACAATGATTTTC
CAGAACTGCTCTGTGCTACTATCACAGCCCTGTACCTTTTAGCTTTGACAAGCAATGGACTACTGCTGCT
GGTCATCACCATGGATACCCGGCTCCACGTGCCCATGTATCTTCTGCTCTGGCAACTCTCTCTCATGGAC
CTGCTCCTCACATCAGTCATCACTCCAAAGGCCATACTAGATTATCTTCTCAAAGACAACACTATCTCAT
TTGGGGGATGTGCCCTTCAGATGTTCCTGGCTTTGACACTTGGTACTGCAGAGGACCTCCTTCTGTCCTT
TATGGCCTATGACAGGTATGTGGCCATTTGTCATCCTCTGAACTACACAATTCTAATGAGTCAGAAAGTC
TGCTGTCTCATGATAGCCACTTCATGGAGTCTTGCATCTCTCAGTGCTCTAGGATATAGCATGTATACCA
TGCAATATCCTTTCTGCAAGTCCAGGCAGATCAGGCATCTCTTCTGTGAGATTCCTCCCTTGCTAAAGCT
GGCCTGTGCAGACACCTCCACATATGAACTCATGGTTTATCTGATGGGTGTGACCTTGCTCTTTCCAGCT
CTTGCTGCCATCCTGGCTTCCTACTCACTAATTCTATTCACTGTGCTGCACATGCCCTCTAATGAGGGCA
GGAGGAAAGCCCTTGTCACCTGCTCTTCCCATCTGACTGTGGTTGGGATGTGGTATGGAGGTGCTATTGT
CATGTATGTATTACCCAGTTCCTTCCACAGCCCCAAACAAGACAATATCAGCTCAGTTTTTTATACAATT
TTCACTCCGGCTTTGAATCCCCTCATCTACAGCCTAAGGAATAAGGAGGTCACTGGAGCTTTGAGGAGAG
TCCTTGGGAAAAGGTTGTCAGTACAGTCTACATTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR213252 representing NM_001011749
Red=Cloning site Green=Tags(s)

MEVCNSTLRSGFILMGILDDNDFPELLCATITALYLLALTSNGLLLLVITMDTRLHVPMYLLLWQLSLMD
LLLTSVITPKAILDYLLKDNTISFGGCALQMFLALTLGTAEDLLLSFMAYDRYVAICHPLNYTILMSQKV
CCLMIATSWSLASLSALGYSMYTMQYPFCKSRQIRHLFCEIPPLLKLACADTSTYELMVYLMGVTLLFPA
LAAILASYSLILFTVLHMPSNEGRRKALVTCSSHLTVVGMWYGGAIVMYVLPSSFHSPKQDNISSVFYTI
FTPALNPLIYSLRNKEVTGALRRVLGKRLSVQSTF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001011749
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001011749.1, NP_001011749.1
RefSeq Size 948 bp
RefSeq ORF 948 bp
Locus ID 257902
Cytogenetics 7 E3
MW 35.1 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001011749" in other vectors (4)
SKU Description Size Price
MC213297 Olfr704 (untagged) - Mouse olfactory receptor 704 (Olfr704), (10ug) 10 ug
$330.00
MG213252 Olfr704 (tGFP-tagged) - Mouse olfactory receptor 704 (Olfr704), (10ug) 10 ug
$500.00
MR213252L3 Lenti ORF clone of Olfr704 (Myc-DDK-tagged) - Mouse olfactory receptor 704 (Olfr704) 10 ug
$600.00
MR213252L4 Lenti ORF clone of Olfr704 (mGFP-tagged) - Mouse olfactory receptor 704 (Olfr704) 10 ug
$600.00

Plasmid Map for MR213252

Download sequence for

Cloning scheme for MR213252

Circular map for MR213252

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