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Gm14085 (NM_001085518) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212975
Gm14085 (Myc-DDK-tagged) - Mouse predicted gene 14085 (Gm14085)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $614.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gm14085
Synonyms OTTMUSG00000015615
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212975 representing NM_001085518
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGTCAGAGGGAAGAAAGTCTGTTTCCCAGGCCAGAGTGGAGAATGGCATGGAGAACCCAGGTC
TCGAGCTCATGGAAGGTGGAAATCTTGCGCAAAGAAACACACTAGAGGAAGTGACACAGGGACTCAGCCT
GGAGGATGGCTTGGGTCATTCCTCTCTCTGGAGCAGGAGGATTTTTCAGCCTTTCACGAAGGCGAGAAGT
TTCTTTGAAAGACATGCTGGTTTATTCAGAAAGATCCTGCTTGGTCTCTTGTGTTTAGCTTATGCTGCCT
ACTTCCTGGCAGCGTGCATCCTGAACTTCCAGAGGGCACTGGCCTTGTTTGTCATCACCTGTCTTGTGTT
CTTCATTTCTGCTTACCACTTTCTGGAAAAATTCCCTGCTAAAAAATTAATAAGATGTCTAAGTTGCTTT
AGAAACTTCTGCCTGAGACGTTGTCTGAAGTGGGTGTTGCCAGGAGCCTCTGTAGTTAGCCAGATCTTGT
GGCTGGTTCTCGACACAGCTCAAAGGCCAGAGCAGCTGATCTCCTTTGCAGGAATCTGTATGTTCATCCT
CATCCTCTTTGCCTGCTCCAAACACCACAGTGCGGTATGCTGGAGGACGGTGTTTTGGGGCCTGGGCCTT
CAGTTCATCTTTGGGATTTTGGTCATCAGAACTGAACCTGGATTTAATGCATTTCAATGGCTGGGAGATC
AGATCCAGATTTTCCTGGCTTATACTGTGGAAGGATCCAGTTTTGTTTTTGGTGATACACTGGTCTACAA
TGTTTTTGCTTTTCAGTCTCTGCCAATCATTATTTTCTTCGGATGTGTGATGTCTATTCTTTACTACCTG
GGCCTTGTGCAGTGGGTGATTCAGAAGATTGCCTTGTTTCTTCAAAGCAAGATGGGCACCACTGCTCCAG
AAACCCTGGCCATGGCAGGAAACATCTTTGTGGGGCTGACAGAAGTACCTCTGCTCATCCGTCCCTACCT
TGCAGACATGACCATCTCTGAAATCCATGCAGTCATGACTGGAGGCTTTGCTACCATAGCAGGCACAATG
TTGGGAGCCTTCATATCCTTTGGGATTGATGCATCATCCTTGATTTCTGCCTCAGTGATGGCTGCCCCTT
GTGCACTTGCCCTGTCCAAACTGGTATATCCAGAAGTCAAAGAGTCCAAGTTCAAGAGCAAGGAGGGACT
GAAGCTGCCTCGAGGGGAAGAGAGGAATATCCTGGAAGCTGCAAGCAATGGAGCCACAGAAGCCATAAGC
CTTGTTGCTAATGTTGCAGCCAACCTGATTGCCTTTCTGGCTGTATTGGCCTTCGTCAATGCTACCCTCT
CCTGGCTGGGGGAAATGGTGGATTTCCGTGGACTCAGTTTCCAGGTCATCTGCTCCTATATCCTAAGGCC
CATGGCTTTCATGATGGCTGTACAGTGGACAGACTGCCTTCTGGTGATTGAGATAGTGGGAGTCAAATTC
TTCATAAATGAATTTGTGGCCTACCAGCAACTGATTCAGTACAAGAACAAACGTCTCTCTGGAGTAGAAG
AGTGGATCAATGGCGAGAAACAGTGGATTTCTGTGAAAGCTGAAATCATTACAACATTTTCCCTCTGTGG
ATTTGCCAATCTTAGTTCCATAGGAATCACACTGGGAGGCTTGACATCCATGATAACCCAGAGGAAGAGT
GACTTGTGCAAGATTGTGGTCAGGGCCCTCTTCACAGGTGCCTGTGTTTCCTTTATCAGTGCCTGCATGG
CAGGAATCCTCTATGTTCCCAGAGGCGCTGAAACTGACTGTGTCTCATTCCTAAACACAAATTTCACCAA
TAGGACCTATGAGACATATGTGTGCTGCAGAGAGCTTTTCCAGAGTACTTCGCTGAATGGCACCAACATG
CCTTCTTTCTCAGGCCCCTGGCAAGACAACGTGTCCAGCCTCAGGAATCTTGCTAGCTGCTGTGACCTCT
ATACCAGCACTGTGTGTGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212975 representing NM_001085518
Red=Cloning site Green=Tags(s)

MEKSEGRKSVSQARVENGMENPGLELMEGGNLAQRNTLEEVTQGLSLEDGLGHSSLWSRRIFQPFTKARS
FFERHAGLFRKILLGLLCLAYAAYFLAACILNFQRALALFVITCLVFFISAYHFLEKFPAKKLIRCLSCF
RNFCLRRCLKWVLPGASVVSQILWLVLDTAQRPEQLISFAGICMFILILFACSKHHSAVCWRTVFWGLGL
QFIFGILVIRTEPGFNAFQWLGDQIQIFLAYTVEGSSFVFGDTLVYNVFAFQSLPIIIFFGCVMSILYYL
GLVQWVIQKIALFLQSKMGTTAPETLAMAGNIFVGLTEVPLLIRPYLADMTISEIHAVMTGGFATIAGTM
LGAFISFGIDASSLISASVMAAPCALALSKLVYPEVKESKFKSKEGLKLPRGEERNILEAASNGATEAIS
LVANVAANLIAFLAVLAFVNATLSWLGEMVDFRGLSFQVICSYILRPMAFMMAVQWTDCLLVIEIVGVKF
FINEFVAYQQLIQYKNKRLSGVEEWINGEKQWISVKAEIITTFSLCGFANLSSIGITLGGLTSMITQRKS
DLCKIVVRALFTGACVSFISACMAGILYVPRGAETDCVSFLNTNFTNRTYETYVCCRELFQSTSLNGTNM
PSFSGPWQDNVSSLRNLASCCDLYTSTVCA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001085518
ORF Size 1980 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001085518.1, NP_001078987.1
RefSeq Size 3740 bp
RefSeq ORF 1983 bp
Locus ID 381417
Cytogenetics 2 E5
MW 73.1 kDa
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Documents
Resources
"NM_001085518" in other vectors (4)
SKU Description Size Price
MC220213 Gm14085 (untagged) - Mouse predicted gene 14085 (Gm14085), (10ug) 10 ug
$677.00
MG212975 Gm14085 (tGFP-tagged) - Mouse predicted gene 14085 (Gm14085), (10ug) 10 ug
$814.00
MR212975L3 Lenti ORF clone of Gm14085 (Myc-DDK-tagged) - Mouse predicted gene 14085 (Gm14085) 10 ug
$914.00
MR212975L4 Lenti ORF clone of Gm14085 (mGFP-tagged) - Mouse predicted gene 14085 (Gm14085) 10 ug
$914.00

Plasmid Map for MR212975

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212975
Circular map for MR212975

file-alt Citations

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