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9230110C19Rik (NM_199017) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212718
9230110C19Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9230110C19 gene (9230110C19Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "9230110C19Rik or NM_199017"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 9230110C19Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212718 representing NM_199017
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGGGGAGCCACGGGATGGAGGCGGCTACTACTTCAGATTCCTGCCTCATAGAACCTTCTCGT
CGCTGAGCGCCCGGGAGATCACCAGCCGCCTCCGCCAGTGGTCCATGCTGGGCAGAATCCAGGCGCAGGC
GTTCAGCTTCGACCAGACGTTCCAGCCCTACCAAAAGGATGATTTTGTCATGGCTTTTTTCAAAGACCCA
AATGTTATTCCAAATTTGCAGTTACTCTCAGATTCTTCTGGACAGTGGACCACATTAGGATCTGAAGTGA
AAAAAATTGAAGCTATAAATGTTCCATGCACACAACTTTCAATGTCATTTTTCCAACGCTTATACGATGA
AAATATTGTACGAGAAAGTGGACACATTGTCAAATGCCTAGATTCTTTCTGTGATCCGTTTCTCATTTCA
GACGAACTGAGAAAAGTTTTGCTCATGGAAGACTCTGAAAAATATGAAGTCTTTAGCCCAGTGGAAAGGG
AAGAGTTTCTGTTCTGTCTTTTTAAGCATCTCTGCCTCGGAGGGTCCCTTTGTCAGTATGAAGATGTGCT
AAAGCCATATCTGGAGACAGCTAAGCTGATCTATAAGGATCTGGTGAGTGTTCGGAAACATCCTCGAACC
AAGGAAATACAAATTACCTCTTCTGTCTTTAAAGTAAAAGCCTACGACTCTGTTGGTGTGTGCTACCCTT
CACCTAAAGAGCATGAGCAGACATTCTCGTATTTTGTTGTGGACCCCATCAAGCGTCATGTGAATGTCCT
GTACCACTGCTATGGTGTAGGGCACATGGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212718 representing NM_199017
Red=Cloning site Green=Tags(s)

MAAGEPRDGGGYYFRFLPHRTFSSLSAREITSRLRQWSMLGRIQAQAFSFDQTFQPYQKDDFVMAFFKDP
NVIPNLQLLSDSSGQWTTLGSEVKKIEAINVPCTQLSMSFFQRLYDENIVRESGHIVKCLDSFCDPFLIS
DELRKVLLMEDSEKYEVFSPVEREEFLFCLFKHLCLGGSLCQYEDVLKPYLETAKLIYKDLVSVRKHPRT
KEIQITSSVFKVKAYDSVGVCYPSPKEHEQTFSYFVVDPIKRHVNVLYHCYGVGHMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199017
ORF Size 801 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199017.3
RefSeq Size 1592 bp
RefSeq ORF 804 bp
Locus ID 234912
UniProt ID Q8CC70
Cytogenetics 9 A1
MW 30.9 kDa
Summary Cilium- and flagellum-specific protein that plays a role in axonemal structure organization and motility. May play a role in outer and inner dynein arm assembly.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_199017" in other vectors (4)
SKU Description Size Price
MC212998 9230110C19Rik (untagged) - Mouse RIKEN cDNA 9230110C19 gene (9230110C19Rik), (10ug) 10 ug
$330.00
MG212718 9230110C19Rik (tGFP-tagged) - Mouse RIKEN cDNA 9230110C19 gene (9230110C19Rik), (10ug) 10 ug
$500.00
MR212718L3 Lenti ORF clone of 9230110C19Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 9230110C19 gene (9230110C19Rik) 10 ug
$600.00
MR212718L4 Lenti ORF clone of 9230110C19Rik (mGFP-tagged) - Mouse RIKEN cDNA 9230110C19 gene (9230110C19Rik) 10 ug
$600.00

Plasmid Map for MR212718

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212718
Circular map for MR212718

file-alt Citations

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