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Fbxl18 (NM_001033312) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212415
Fbxl18 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 18 (Fbxl18)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $647.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxl18
Synonyms AI427077; B130019G13Rik; C330021B20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212415 representing NM_001033312
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCAGTGAGGAGGAGGCAGCGGCAGAGGCAGCAGGTGACACCCACCTGCTTGGCTTCTCCGATGAGA
TCCTCCTACACATCCTGAGCCATGTCCCCAGCACGGACCTGGTTCTCAGTGTGCGGCGCACCTGCAGGAA
ACTGGCAGCCCTGTGCCTGGACAAGAGCCTGGTGCACACCGTGCTGCTGCAGAAGGACTACCAGGCCAGT
GAGGAGAAGGTGAAGCAACTGGTGAAGGAGATTGGCCGGGAGATCCAACAGCTGAACATGGCAGGCTGCT
ATTGGTTGTCTGGCTCCACCATCGAGCACGTGGCCCGGTGTCACAGCCTGGTGAAGGTGAACTTGTCAGG
TTGCCACCTCACCTCGCTGCGGCTGTCCAAGGTGCTCTCGGCCCTGCAGCACCTGCGCTCATTGGCCATT
GACGTGAGCCCCGGCTTCGACGCCAGCCAGCTGAGCAGTGAGTGCAAGGCCACGCTAAGCCGCGTGCAGG
AGCTCAAGCAGACACTCTTCACGCCGTCCTACGGGGTGGTGCCCTGCTGTGCCAGCCTGCAGAAGCTGCT
GCTCTACTTCGAGATCCTGGACCGCACCCGGGAGGGGGCCGTCCTCTCGGGCCAGCTCATGGTGGGCCAG
AGCAACGTGCCCCACTACCAGAACCTGCGTGTCTTCTATGCACGCCTGGCTCCTGGCTACATCAACCAGG
AGGTGGTGAGACTCTACCTGGCCGTGCTCAGCGACCGCACGCCTGAGAACCTGCATGCCTTCCTCATCTC
CGTCCCTGGCAGCTTTGCAGAGAGCGGGGCCACCAAAAACTTGCTGGACTCCATGGCCCGCAACGTGGCC
CTGGATGCACTGCAGCTGCCCAAGTCCTGGCTCAATGGCTCCACCCTCCTCCAGCATATGAAATTCAACA
ATCCCTTCTACTTCAGCTTCAGCCGGTGTACGCTGTCAGGGGGTCACCTGATTCAGCGGCTCATCAATGG
CGGGAAGGACCTGCGTAGCCTGGCCAGCCTCAACCTCAGCGGCTGTGTGCATTGCCTGTCTGCGGACTCG
TTGCTGCGCAAGGCCGAGGACGACATTGACAGCAGCATCCTGGAGACTCTGGTGGAGTCCTGCTGCAACC
TGCACCACCTCAATCTCTCTGCCGCCCATCACCACAGCTCCGACGGCCTGGGCCGCCACCTCTGCCAGCT
CCTGGCTCGGCTCTGCCACCTGCGTTCCCTGTCCCTGCCTGTCTGCTCTGTGGCTGACTCGGCACCCAGG
CCTGACCGTGCGCCCGCCCCGCCAGCCATGCACGCAGTACCCCGTGGATTTGGCAAGAAGGTGCGCATTG
GGGTGCAGACCTGTCCCAACCCCTTCGTGGGCCAGTCGGCCCCACAGCCTGCCTCTGTGTTCTGGTCTCT
GCTGAAGAAGCTCCCATTTCTGGAGCACCTGGAACTGATCGGGTCCAATTTCTCCTCAGCCATGCCCCGC
AATGAGCCTGCCATCCGCAACTCCCTCCCACCCTGCAGTCGGGCACAGAACGTGGGGGACTCAGAGGTGG
CCGCCATTGGCCAGCTGACCTTCCTGCGGCACCTGACTCTAGCCCAACTGCCAGGCATGCTCACGGGCTC
CGGACTCGTGAGCATCGGTCTCCAGTGCCAGCACCTGCAGTCTCTATCGCTGGCCAACCTGGGCATGATG
GGGAAGGTGGTGTACATGCCTGCTCTTGCTGACATGCTGAAGCACTGCAAGCGGCTGAAGGACCTCAGGT
TGGAGCAGCCCTACTTCAACGCCAACGCTCAGTTCTTCCAGGCACTGGGCCAGTGCTCGTCTCTGCAGCG
CCTGTGCCTGGTATCTCGCAGCGGCACCCTGCAGCCAGATGCCGTGCTGGCCTTTATGGCCCGTTGCCTG
CAGGTCGTCATGTGTCACATGTTCACCGGAGAATCCCTTACCACCTGCAAGAGCCTGCAGCAGTCGCTCC
TCCGGAGTTTCCAGGCCGAGAGGCCCGCGTTGAACGTCGTCATCTTCCCCCTGCTCCACGAGGGCCTGAC
AGATGTCATCCGAGATGTCCCCATGCTGCACCTGGATGAGATCACCTTGTTCAAAAGCCGTGTGGCTGAG
GAACCGCCCAACCTATGGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212415 representing NM_001033312
Red=Cloning site Green=Tags(s)

MSSEEEAAAEAAGDTHLLGFSDEILLHILSHVPSTDLVLSVRRTCRKLAALCLDKSLVHTVLLQKDYQAS
EEKVKQLVKEIGREIQQLNMAGCYWLSGSTIEHVARCHSLVKVNLSGCHLTSLRLSKVLSALQHLRSLAI
DVSPGFDASQLSSECKATLSRVQELKQTLFTPSYGVVPCCASLQKLLLYFEILDRTREGAVLSGQLMVGQ
SNVPHYQNLRVFYARLAPGYINQEVVRLYLAVLSDRTPENLHAFLISVPGSFAESGATKNLLDSMARNVA
LDALQLPKSWLNGSTLLQHMKFNNPFYFSFSRCTLSGGHLIQRLINGGKDLRSLASLNLSGCVHCLSADS
LLRKAEDDIDSSILETLVESCCNLHHLNLSAAHHHSSDGLGRHLCQLLARLCHLRSLSLPVCSVADSAPR
PDRAPAPPAMHAVPRGFGKKVRIGVQTCPNPFVGQSAPQPASVFWSLLKKLPFLEHLELIGSNFSSAMPR
NEPAIRNSLPPCSRAQNVGDSEVAAIGQLTFLRHLTLAQLPGMLTGSGLVSIGLQCQHLQSLSLANLGMM
GKVVYMPALADMLKHCKRLKDLRLEQPYFNANAQFFQALGQCSSLQRLCLVSRSGTLQPDAVLAFMARCL
QVVMCHMFTGESLTTCKSLQQSLLRSFQAERPALNVVIFPLLHEGLTDVIRDVPMLHLDEITLFKSRVAE
EPPNLWW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033312
ORF Size 2121 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033312.3, NP_001028484.2
RefSeq Size 2831 bp
RefSeq ORF 2124 bp
Locus ID 231863
Cytogenetics 5
MW 77.7 kDa
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"NM_001033312" in other vectors (4)
SKU Description Size Price
MC220911 Fbxl18 (untagged) - Mouse F-box and leucine-rich repeat protein 18 (Fbxl18), (10ug) 10 ug
$713.00
MG212415 Fbxl18 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 18 (Fbxl18), (10ug) 10 ug
$847.00
MR212415L3 Lenti ORF clone of Fbxl18 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 18 (Fbxl18) 10 ug
$947.00
MR212415L4 Lenti ORF clone of Fbxl18 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 18 (Fbxl18) 10 ug
$947.00

Plasmid Map for MR212415

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212415
Circular map for MR212415

file-alt Citations

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