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4930562C15Rik (NM_030192) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212245
4930562C15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930562C15 gene (4930562C15Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $997.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 4930562C15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212245 representing NM_030192
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTTCTCAATCACATTCACCGAGCTGGTCAACGTAGCCATCCCACAGTGTGGGGTCGTGAACTTCA
AGGCCCTTCATCTCCTGCTCCAGGGCATCCTGGAGCACATCCAAATCACTGAGCTCAAGAAAGTTCTTTC
AGGGGAAGAGGACTTCCTTCAATCCTCACCGGCTGTGTTTATACCCAGGGAGGGAGATGCCCAGCCTGTC
ATCAACCCAATGAAGAGGCTTAGCAACATCTTTGACCAAGTAGTAGACCGAATCGAAAAGATAGAGTCCA
AACTGGCTGAGATGCAGGACATACCCACCACCTCACAGCTGGTAGAGGAGAGCTATGGGGACAAGCGGCC
TGCTGAGGAGATGTGGAATAATATCAAGATACAGAAGAGGATAGAGGGCAACGAGAAAGCCACAGAAAAG
TTTACCAGGACTCTGCAGGATCTGCTCAACGACCTTCATGTCCTCAAGGGCTCTGTTGAGACTGTTCAGA
AGGATGTGGACACGATCAAGTTTATATTTGAAAAGGTAAACCCACAAAAATTGGAACTTCTCGGTGATGA
CTTGAAAACACAGAGCCGGAAGTTGGGTGCCCTGCAGCGGGAAGTGGTTACCCTCCAGAACAAGGTTCGA
GCTGTCCCTCAACCTGAGGAGATGGTGCTCTGGAGTGGCCTCCATGAGGCCATGTTCAGTCCGGAACAGC
AGAAGCTGGAAGTGGAGCCGTCCGGAGTCTGGCAGAGCATGGTCTCTCTGCCAGGGTCTCCTTTGGAGCA
GCCTGAGGATGCTGGCTACATCCATATCCCCATCCACAGTGCCACCCCTGTGCCCACCATGGTGCAGTTA
GGAACCACAGGAGCCCTCAGAGAGGAGGAGCTCACTCGGGCTGTAGAACTTGCTGACTCCCAGAGGCCAG
AGCTGGCTCAGGCCTTTGTGTCTCAGACACAGAGGCTAGAACGGGCTCAGGCCTCTGTGTCTCAGACACA
GAGGCTAGAGCGGGCTCAGGCCTCTGTGTCTCAGACACAGAGGCTAGAGCGGGCTCAGGCCTCTGTGTCT
CAGACACAGAATCTGAGACAGACTGCCCCTTTCCCTTTCCCTGTCCCTGGTTTTGTGCCTGGGCCTGATT
TCATGCCAGTCTATGGGCCTAGACCTGGAATGGCTACCCCAAGCGCATGGGCCTTACCAAGAGGCTTGGC
TAGGGGTAACTATTGGCCTATATGGGGTATAGGTCCCTACCAGCCTGGCCAAGGTCCACTTCAGCCTATG
GGCCCGATGCCCAGGGTTCCCTTCCCAGCCATGGGAAGTGACATACCATGGACTCAGCCACTGCCACAAG
GGACCCAGTGGTACCCAAATATTGAAGAAAGAGGGCCTGAATATGAGGAACAATACCCGGAAGGCTATCA
GGAGGAGATGCCCATGGATGAGGCTCCCCAGGAAGAGATGGCTGTGTATGGGGCTCTTGAGGAAGAGATC
CCTACGGAAGGGGCTCCCCAGGATGGGATTCCCAAGGGAAGAGCTCGGGAGAGGATTTCCAAGGAAAAGG
TCCCCAAGTACAGAACCCCCAAGGAGAGGGTTTCCTATCACAAAGCCTCCAAGGATGTGAGCCCCGAAGA
CACAGGCACCAAAGATGAGGCTCCTGAAGTCAAAGTTCTCAAAAAGGTGGTCTACAAAGACAGACCTCAG
AAGGACAAGGCCCCGGAACCACGCCGTAAGGGCCCTTCATCTGCCATAAAGAAGCTGAGGTCTGCTATTG
CCTCAGCTGCAGCAGCTGCGGCTGCCTACGCAGCCCATGCAAACTCAGCTGCCCAGCTTGCCAAAGATGC
TGTAAAGGCCATCCAGGACGTTCCTGCAAACCAGCTGGCTGCCAAGGCAGCATTAATTGCATCATCAGGG
CCTTTAGGAGCCTTTGCAGATTTCCTGGGTGCAGGATTTGGCCATGGGGCCACTTCCAACACACCCTTCG
ATGAGGGTGAGTTGGAAGAGTTTCCTGAAGAGTTTGCAGCACCCTTTAACCCCTTCACATCCAAACCAGT
CCTGTCCCAGGCCATGATAAGTGCCACGCAGGCTACGAGTCCTGAAGAAAAGAAAAAGGCCGTGCAGTAC
TCCATGGGTCACATTGCCCAGATGCCCAGCAGACATAACTCCCTGAAAGAAGAGTTTACAAATCTGTCAA
CCACCTTAAACCAACGCTTAAATTACTTAGCTAACATGGGAAGCTCTGGCGTGCTTGGTAGCACAGTAAA
TGTTCTGGAAGAAAAGATTATTAACCTCCAGAAGGCCAGGCTGCAGGAAGAAGAGCTGGAGAGAGTTTGG
GGCCACCAGATAGATACGATGAAGAGTCACTACATGGTGCTGGACAGGGCAGTGGAAAGGCTCCAGATTC
GCATGGATGACTTGAAGATCCTCAAGGCAGAAATCGAAAGGCTGGACCTGGTTAAGGCAGATAAAAATGT
GATGGATCTGGAGCTGAATGAAAAAGCTAACAGGGACGCATTGGCAAGCAAGGCAAACAGAGTGGACTTG
GAGACGGTGGCGATGGAGCTGAATGAGATGATTCACAGCATGCTCTTGAAGATCACAAACTATGAGTCTG
ACTGGAAGAAGGCTCTGAAACATCTCAGGAAGGACCTGAACACTAAGCTAGTCCAGAGCGATCTGAATAG
CCTAAAGAAGGACATAGAAGAGGTCTGGAAGGTGGTCCGGAAGCTTCTGCTAGAAGGTCTCCGATTCGAC
CCTGACAGCGCTGCAGGCTTTAAGAAGAAATTGTTCGAGAGGGTAAAGTGCATCTCCTGCGACAGGCCCG
TAGAGATGATGACTGGCCCACAGCTGATCACCATCCGCAACACCCATGGGCTGTCCCGAATAAGGCCAGC
CAGTGCCAACAGCTATGAGTATCTGCAGCGGCAGCTGATAAGGGAACAACAGCAGCATCTGCATTTCCAG
AACTTTGGAGTCCATGAGGAGGGTCAGGGCTTCCAGAAGGACTGGGGTGATGGGCCCCGAAATGAGAACA
CCTTGAAGCACAAGTCACATGACCTGTCAACACTCTATCCTTATGGGGACCCTGAGCTGATGAACTATGA
CACTGCTGAGGTGGACATCTTGGGGGTAGATGGAGTTCTGTACAAAGGCCGAATGAGCAGCCAGTTTGGG
ACTCGGACTGGGGAGAAGGACATTGCAGCTGTGAAAGTTTCATATTACACCATTCCAAACCTGACTGATC
GTGTGCGCCCAGGATCCTTACTTGCTCCGGGCTATCCACCCTTGAGCCCCCGCACCAGTATCACCTCTGC
CACCACTCTACACTCTGCCACCAACACCACGACGGCACGGCCACCATCCCTGCCACCAGTGCCACAGCTA
CCACCACTGATCCCTGTCTCAAGGGATACCCAAGAGGCCCCTGGATCTGCCAAGCGTTTGAAATCTCCAC
GGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212245 representing NM_030192
Red=Cloning site Green=Tags(s)

MSFSITFTELVNVAIPQCGVVNFKALHLLLQGILEHIQITELKKVLSGEEDFLQSSPAVFIPREGDAQPV
INPMKRLSNIFDQVVDRIEKIESKLAEMQDIPTTSQLVEESYGDKRPAEEMWNNIKIQKRIEGNEKATEK
FTRTLQDLLNDLHVLKGSVETVQKDVDTIKFIFEKVNPQKLELLGDDLKTQSRKLGALQREVVTLQNKVR
AVPQPEEMVLWSGLHEAMFSPEQQKLEVEPSGVWQSMVSLPGSPLEQPEDAGYIHIPIHSATPVPTMVQL
GTTGALREEELTRAVELADSQRPELAQAFVSQTQRLERAQASVSQTQRLERAQASVSQTQRLERAQASVS
QTQNLRQTAPFPFPVPGFVPGPDFMPVYGPRPGMATPSAWALPRGLARGNYWPIWGIGPYQPGQGPLQPM
GPMPRVPFPAMGSDIPWTQPLPQGTQWYPNIEERGPEYEEQYPEGYQEEMPMDEAPQEEMAVYGALEEEI
PTEGAPQDGIPKGRARERISKEKVPKYRTPKERVSYHKASKDVSPEDTGTKDEAPEVKVLKKVVYKDRPQ
KDKAPEPRRKGPSSAIKKLRSAIASAAAAAAAYAAHANSAAQLAKDAVKAIQDVPANQLAAKAALIASSG
PLGAFADFLGAGFGHGATSNTPFDEGELEEFPEEFAAPFNPFTSKPVLSQAMISATQATSPEEKKKAVQY
SMGHIAQMPSRHNSLKEEFTNLSTTLNQRLNYLANMGSSGVLGSTVNVLEEKIINLQKARLQEEELERVW
GHQIDTMKSHYMVLDRAVERLQIRMDDLKILKAEIERLDLVKADKNVMDLELNEKANRDALASKANRVDL
ETVAMELNEMIHSMLLKITNYESDWKKALKHLRKDLNTKLVQSDLNSLKKDIEEVWKVVRKLLLEGLRFD
PDSAAGFKKKLFERVKCISCDRPVEMMTGPQLITIRNTHGLSRIRPASANSYEYLQRQLIREQQQHLHFQ
NFGVHEEGQGFQKDWGDGPRNENTLKHKSHDLSTLYPYGDPELMNYDTAEVDILGVDGVLYKGRMSSQFG
TRTGEKDIAAVKVSYYTIPNLTDRVRPGSLLAPGYPPLSPRTSITSATTLHSATNTTTARPPSLPPVPQL
PPLIPVSRDTQEAPGSAKRLKSPRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030192
ORF Size 3435 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030192.1, NP_084468.1
RefSeq Size 3829 bp
RefSeq ORF 3438 bp
Locus ID 78809
UniProt ID E9QMW4
Cytogenetics 16 2.47 cM
MW 128.1 kDa
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Documents
Resources
"NM_030192" in other vectors (4)
SKU Description Size Price
MC223745 4930562C15Rik (untagged) - Mouse RIKEN cDNA 4930562C15 gene (4930562C15Rik), (10ug) 10 ug
$1,097.00
MG212245 4930562C15Rik (tGFP-tagged) - Mouse RIKEN cDNA 4930562C15 gene (4930562C15Rik), (10ug) 10 ug
$1,197.00
MR212245L3 Lenti ORF clone of 4930562C15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930562C15 gene (4930562C15Rik) 10 ug
$1,297.00
MR212245L4 Lenti ORF clone of 4930562C15Rik (mGFP-tagged) - Mouse RIKEN cDNA 4930562C15 gene (4930562C15Rik) 10 ug
$1,297.00

Plasmid Map for MR212245

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212245
Circular map for MR212245

file-alt Citations

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