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D5Ertd577e (NM_177187) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212214
D5Ertd577e (Myc-DDK-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 577, expressed (D5Ertd577e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "D5Ertd577e or NM_177187"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D5Ertd577e
Synonyms E330023J07Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212214 representing NM_177187
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGTCAGACCCCACCCACACTCCAGAAGCTAGCAAGGCAGACACTGCTGAGAGATGAGGCTTTGG
CCATATCCTCTCTGGAGGAGCTGCCAGCTGTGGTCTTCCCACCACTGTTCCAAGAGGCCTTTGCTGGCAG
ACTCAACAAGCTCATAAAGGCAATGGTGGCAGCCTGGCCTTTCCACGATCTCCCTGTGGGGCCATTGATA
AATATGCATAACCTGGAAACCTTGCAAGCTCTGCTAGATGGAGTAGACATGAGACTAACAAGAAAATTTC
ACCCCTGGAGGCCAAAACTTCAGGTTCTCGACATGAGAAACGTGGACCATGTCTTCTGGAACATATGGAG
CGATGCAAATGACAGTGACTCTGATGCAGAGACCTTGGATGAAAAGCAAGTAGTGAAGGCCCCTCGCAGA
TATGCACTGAGGCAGCGTCTGAAGATCATAGTTGATCTGTCAATCAGTTCTCAGCTCAATGAACAAAAAG
CATATTTCTTGAATTGGGCCAAGCAGAGAAAGGGGTCCATAAATTTCTGCTGTACAAAGATGAAGATCTG
GGATGCACCAGACGAAGTTATCAGAGAAATCATGAATGTTTTCCATCCAGAGCACATTACAGAATTAGAA
CTGTATACCGACTGGACTCTGTTGCGGCTGGCACATTTTGCTCCCTACATTGGGCAGATGAAAAATCTTG
AAAGAGTCTTCCTGGCACCACTCCACAAGAATACCTCCCCTATTATGAATTTGACAAGGGACTCAAAAGT
CAAGTGTATTAAAAAAATTATTTCTCAGTTTTCCAAATTCAACTGTCTCCAACATGTCTTCATGAAACGT
GTCCATTTTCTCAGAGACCACCTGCATCAGATACTAGGGTGCCTGAGGACGCCCTTGCAGACCCTCTCCA
TCACTCACTGCCTAATTTCACAGACAGACTTGGATTCCTTTTCCTGCTGTCACAACCTTTTTAAGTTAAA
AAATCTGGAAATCAGAGGAGTGACATTATTTGCTCTCGATCTTATGCCTCTGAGAGGTCTCCTAGGAAAA
CTGGCAGGTACTCTTGAGTCTCTGGATTTTCAGTGGTGTAGCATGAAGGACTCCCAGCTCATTGTCCTCT
TACCTGCCCTCAGTCAATGCTCTCAGCTCAACCAGATCAACTTCTACAGCAATGACTTCTCCATGGCCAT
CCTGAAGGACCTTTTGCAGCACACAGCCAACTGGAGCAAGATGAATGTGGAACAATACCCTGTCCCTCTG
GAGTGCTATGATGCATTGGGTCATGTCTCCAGAGAAAGATTTGTGGAACTTTGTCAGGAGCTCATGGATA
CCCTCAGGGCCAAAAGAGAGCCTAAGAGCATATCTTTTGCAACAAATGTCTGCCAAAATTGTGGCAAGAC
CTGTGTCTATGGCCAGGGGGCAAGACTTTGTTCCTGTTTGCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212214 representing NM_177187
Red=Cloning site Green=Tags(s)

MSGQTPPTLQKLARQTLLRDEALAISSLEELPAVVFPPLFQEAFAGRLNKLIKAMVAAWPFHDLPVGPLI
NMHNLETLQALLDGVDMRLTRKFHPWRPKLQVLDMRNVDHVFWNIWSDANDSDSDAETLDEKQVVKAPRR
YALRQRLKIIVDLSISSQLNEQKAYFLNWAKQRKGSINFCCTKMKIWDAPDEVIREIMNVFHPEHITELE
LYTDWTLLRLAHFAPYIGQMKNLERVFLAPLHKNTSPIMNLTRDSKVKCIKKIISQFSKFNCLQHVFMKR
VHFLRDHLHQILGCLRTPLQTLSITHCLISQTDLDSFSCCHNLFKLKNLEIRGVTLFALDLMPLRGLLGK
LAGTLESLDFQWCSMKDSQLIVLLPALSQCSQLNQINFYSNDFSMAILKDLLQHTANWSKMNVEQYPVPL
ECYDALGHVSRERFVELCQELMDTLRAKREPKSISFATNVCQNCGKTCVYGQGARLCSCLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177187
ORF Size 1443 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177187.4, NP_796161.3
RefSeq Size 2501 bp
RefSeq ORF 1446 bp
Locus ID 320549
Cytogenetics 5 47.29 cM
MW 55.4 kDa
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Documents
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"NM_177187" in other vectors (4)
SKU Description Size Price
MC216738 D5Ertd577e (untagged) - Mouse DNA segment, Chr 5, ERATO Doi 577, expressed (D5Ertd577e), (10ug) 10 ug
$503.00
MG212214 D5Ertd577e (tGFP-tagged) - Mouse DNA segment Chr 5 ERATO Doi 577 expressed (D5Ertd577e), (10ug) 10 ug
$657.00
MR212214L3 Lenti ORF clone of D5Ertd577e (Myc-DDK-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 577, expressed (D5Ertd577e) 10 ug
$757.00
MR212214L4 Lenti ORF clone of D5Ertd577e (mGFP-tagged) - Mouse DNA segment, Chr 5, ERATO Doi 577, expressed (D5Ertd577e) 10 ug
$757.00

Plasmid Map for MR212214

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212214
Circular map for MR212214

file-alt Citations

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