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D3Ertd254e (NM_001101478) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212170
D3Ertd254e (Myc-DDK-tagged) - Mouse DNA segment, Chr 3, ERATO Doi 254, expressed (D3Ertd254e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
$589.00 $729.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D3Ertd254e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212170 representing NM_001101478
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACAGTTGACCTTCAGGGATGTGGCCATTGATTTTTCTGGAGAGGAATGGGAGTGTCTGGATTCTG
AGCAGCAGAATTTGTATACAGATGTGATGTTGGAAAACTATAGGAACCTCGTTTTCCTGGGTCTTGCTGT
GTCTAAGCCATACCTGGTTACCTGTCTGGAGCAAAGGAAAGAGCCCTGGAATGTAAAGACGCAAAAGACC
ATGGCCGTGTTCCCAGCCATGTTTTCTCATCATAGCCAAGACTTTTCACCAGAGCAGAACATAAAGTATT
CATTCCAGGAACTCATCTGTGGACCTCGTGGACAGTGTGGCTTTGACGCTTTGTACTTCCCAGGAGAAAG
GGACTGTGTTGAGGAGGGTGACATCCTGAGAACCTGCTATAACGGGCACAACCCATTTCTGACAGCTGGC
AACAGCAAAAGCTTTACTCCCAGGAGAGATCGGGCACAAGAGATGGCTCACAAAAACCCTCAGGTGACAC
CAGTTACTTGTGAGGAGCCGTGCGCTTCAGTCATTAAACAGCAGAGTCAGTTTTTGAAGCAGACCCCTTC
TTTCAAAGATAATTTGGAAAATCCACAGAGGGTTCTAGTTAATTCTTCAACTACCGACTTCAACAATTTC
AAGTGTAGCTTTGGGCTGAGCTTTCATTCAAACATTTTTGTAGATGGGGAACTTAAAAATGAAGGAAAAC
ATTCTAAATGTAATCAATTTGAGAACTCCATCATGAAAAATTCGTTAGTATATAATCAACAAATAGGTCT
TCCATGTGCCAAAACACAGAATTCCCATAAGTATGGCAGATTTTTTTCACATCCGGTATCACTGAACCAA
AATTCGAGTACAGATATTTTGGAAATTCAGTATATATGTAAAGAAAATAGGAAGGCCTTCACTGAGGAGT
CCAGCTTAAGTAATCTTCAGGACGTGTATATCGGAGAGACAGTTTATGAGGGTAATGAAAATAATAGTGA
CTTAAACCAAGAGTCAAATCCTGCCAGTCATCAGTCTGCTCAATTGTACAAATGTTACAAATGTGACACA
GTCTTTCATCAGTACTCGGAACTTATTATCCATCAGTACATCCATATTCAAGATCAGATTTCCAAGGATA
TGGATTGCGACACAGCTTTCAGTCAAAGTTCAAGCCTTACGAGAAGTCAAGCTGGAGAAAAACCCTACAA
ATGTAAGGAATGCGGCAAAGCCTTTACCTACTGTTCAAGCCTTAGGCAACATCATAGAATTCATTCTGGA
GTCAAACAGCACAAATGTAAAGACTGTGGCAAAGCCTTCTACCATAAATCACTCCTCACGCAACACCAGA
GCATCCACGCTGGAAAGAAACCCTACTGCTGTAAATTTTGTGGCAAAGCTTTCAATCAAAGATCGACTGT
TACTCAGCACCAGAGAATCCACACTGGAGAGAGGCCCTACCATTGTAAAGATTGCGGCAAAGCATTTCAT
CAAAGATCGAGCCTTAGTCTGCACCAGAGAGTTCATACGGGAGAAAAGCCCTACAAATGCAAAGACTGCG
GCAAAGCCTTCAACCGCAACTCACTCCTCACTCAGCACCAGAGAATTCACACTGGAGAGAGGCCCTACCA
TTGTAAAGACTGTGGCAAAACTTTTAATAAAAAGTCAAGCCTTACTCAACACCAGAGAATCCATACTGGA
GAGAAGCCCTATTCTTGTAAAGACTGTGGCAAAGCTTTTAATCAAAGATCAAGCCTCAGCCTACACCAGA
GAGGCCATACTGGGGAGAAACCCAATAAATGTAATGAATGTGGCAAAGCCTTTAACCGTGTATTTTTCCT
TACTCAACATCAGAAAATTCATACTGGAGAGAAGGCTTACCACTGTAAAGACTGTGGCAAAGCATTTAAG
CAGAGGTCCAGCCTTACTCAGCACCAGAGGGTACACACTGGAGACAAGCCCTATCATTGTAAGCACTGTG
GCAAAGCTTTTACCCAGAGGTCCAGCTTTACACGACACCAGAGGATCCACACTGGAGAGAAGCCCTACAA
GTGTCAAGACTGTGACAAAGCCTTCAGCCGGAACTTACTTCTCATTCAACACCAGAGAATTCACACAGGA
GAGAAGCCTTACCATTGTCAAGATTGTGGCAGAGCGTTTAATCAAAGGTCAAGCCTTACTCAACATCAGA
GGGTTCACACTGGGGACAAGCCCTTCTGCTGTAAAGACTGTGGCAAGGCTTTTACCCAGAGGTCAAGCTT
TAATCGACACCAGAGAGTTCATACTGGAGAGAAGCCCTACAAATGTAAAGAGTGTGATGGCGCCTTTAAA
TCCAGTTCCCACCTTGCCGAACACCAGTGCACCTCATTAGGCAGCTGCTTTGAAGACACAAAATTTCTCC
AATATATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212170 representing NM_001101478
Red=Cloning site Green=Tags(s)

MEQLTFRDVAIDFSGEEWECLDSEQQNLYTDVMLENYRNLVFLGLAVSKPYLVTCLEQRKEPWNVKTQKT
MAVFPAMFSHHSQDFSPEQNIKYSFQELICGPRGQCGFDALYFPGERDCVEEGDILRTCYNGHNPFLTAG
NSKSFTPRRDRAQEMAHKNPQVTPVTCEEPCASVIKQQSQFLKQTPSFKDNLENPQRVLVNSSTTDFNNF
KCSFGLSFHSNIFVDGELKNEGKHSKCNQFENSIMKNSLVYNQQIGLPCAKTQNSHKYGRFFSHPVSLNQ
NSSTDILEIQYICKENRKAFTEESSLSNLQDVYIGETVYEGNENNSDLNQESNPASHQSAQLYKCYKCDT
VFHQYSELIIHQYIHIQDQISKDMDCDTAFSQSSSLTRSQAGEKPYKCKECGKAFTYCSSLRQHHRIHSG
VKQHKCKDCGKAFYHKSLLTQHQSIHAGKKPYCCKFCGKAFNQRSTVTQHQRIHTGERPYHCKDCGKAFH
QRSSLSLHQRVHTGEKPYKCKDCGKAFNRNSLLTQHQRIHTGERPYHCKDCGKTFNKKSSLTQHQRIHTG
EKPYSCKDCGKAFNQRSSLSLHQRGHTGEKPNKCNECGKAFNRVFFLTQHQKIHTGEKAYHCKDCGKAFK
QRSSLTQHQRVHTGDKPYHCKHCGKAFTQRSSFTRHQRIHTGEKPYKCQDCDKAFSRNLLLIQHQRIHTG
EKPYHCQDCGRAFNQRSSLTQHQRVHTGDKPFCCKDCGKAFTQRSSFNRHQRVHTGEKPYKCKECDGAFK
SSSHLAEHQCTSLGSCFEDTKFLQYI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001101478
ORF Size 2388 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001101478.1, NP_001094948.1
RefSeq Size 6625 bp
RefSeq ORF 2391 bp
Locus ID 241944
Cytogenetics 3 17.41 cM
MW 91.7 kDa
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Documents
Resources
"NM_001101478" in other vectors (2)
SKU Description Size Price
MC221737 D3Ertd254e (untagged) - Mouse DNA segment, Chr 3, ERATO Doi 254, expressed (D3Ertd254e), (10ug) 10 ug
$803.00
MG212170 D3Ertd254e (tGFP-tagged) - Mouse DNA segment Chr 3 ERATO Doi 254 expressed (D3Ertd254e), (10ug) 10 ug
$929.00

Plasmid Map for MR212170

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212170
Circular map for MR212170

file-alt Citations

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