OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Dll4 (NM_019454) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR212151
Dll4 (Myc-DDK-tagged) - Mouse delta-like 4 (Drosophila) (Dll4)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $628.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA420161 Dll4 hamster monoclonal antibody, clone HMD4-2, Preservative Free,Purified,RPE
0.25 mg
$645.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Dll4 or NM_019454"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dll4
Synonyms Delta4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212151 representing NM_019454
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACGCCTGCGTCCCGGAGCGCCTGTCGCTGGGCGCTACTGCTGCTGGCGGTACTGTGGCCGCAGCAGC
GCGCTGCGGGCTCCGGCATCTTCCAGCTGCGGCTGCAGGAGTTCGTCAACCAGCGCGGTATGCTGGCCAA
TGGGCAGTCCTGCGAACCGGGCTGCCGGACTTTCTTCCGCATTTGCCTTAAGCACTTCCAGGCAACCTTC
TCCGAGGGACCCTGCACCTTTGGCAATGTCTCCACGCCGGTATTGGGCACCAACTCCTTCGTCGTCAGGG
ACAAGAATAGCGGCAGTGGTCGCAACCCTCTGCAGTTGCCCTTCAATTTCACCTGGCCGGGAACCTTCTC
ACTCAACATCCAAGCTTGGCACACACCGGGAGACGACCTGCGGCCAGAGACTTCGCCAGGAAACTCTCTC
ATCAGCCAAATCATCATCCAAGGCTCTCTTGCTGTGGGTAAGATTTGGCGAACAGACGAGCAAAATGACA
CCCTCACCAGACTGAGCTACTCTTACCGGGTCATCTGCAGTGACAACTACTATGGAGAGAGCTGTTCTCG
CCTATGCAAGAAGCGCGATGACCACTTCGGACATTATGAGTGCCAGCCAGATGGCAGCCTGTCCTGCCTG
CCGGGCTGGACTGGGAAGTACTGTGACCAGCCTATATGTCTTTCTGGCTGTCATGAGCAGAATGGTTACT
GCAGCAAGCCAGATGAGTGCATCTGCCGTCCAGGTTGGCAGGGTCGCCTGTGCAATGAATGTATCCCCCA
CAATGGCTGTCGTCATGGCACCTGCAGCATCCCCTGGCAGTGTGCCTGCGATGAGGGATGGGGAGGTCTG
TTTTGTGACCAAGATCTCAACTACTGTACTCACCACTCTCCGTGCAAGAATGGATCAACGTGTTCCAACA
GTGGGCCAAAGGGTTATACCTGCACCTGTCTCCCAGGCTACACTGGTGAGCACTGTGAGCTGGGACTCAG
CAAGTGTGCCAGCAACCCCTGTCGAAATGGTGGCAGCTGTAAGGACCAGGAGAATAGCTACCACTGCCTG
TGTCCCCCAGGCTACTATGGCCAGCACTGTGAGCATAGTACCTTGACCTGTGCGGACTCACCCTGCTTCA
ATGGGGGCTCTTGCCGGGAGCGCAACCAGGGGTCCAGTTATGCCTGCGAATGCCCCCCCAACTTTACCGG
CTCTAACTGTGAGAAGAAAGTAGACAGGTGTACCAGCAACCCGTGTGCCAATGGAGGCCAGTGCCTGAAC
AGAGGTCCAAGCCGAACCTGCCGCTGCCGGCCTGGATTCACAGGCACCCACTGTGAACTGCACATCAGCG
ATTGTGCCCGAAGTCCCTGTGCCCACGGGGGCACTTGCCACGATCTGGAGAATGGGCCTGTGTGCACCTG
CCCCGCTGGCTTCTCTGGCAGGCGCTGCGAGGTGCGGATAACCCACGATGCCTGTGCCTCCGGACCCTGC
TTCAATGGGGCCACCTGCTACACTGGCCTCTCCCCAAACAACTTCGTCTGCAACTGTCCTTATGGCTTTG
TGGGCAGCCGCTGCGAGTTTCCCGTGGGCTTGCCACCCAGCTTCCCCTGGGTAGCTGTCTCGCTGGGCGT
GGGGCTAGTGGTACTGCTGGTGCTGCTGGTCATGGTGGTAGTGGCTGTGCGGCAGCTGCGGCTTCGGAGG
CCCGATGACGAGAGCAGGGAAGCCATGAACAATCTGTCAGACTTCCAGAAGGACAACCTAATCCCTGCCG
CCCAGCTCAAAAACACAAACCAGAAGAAGGAGCTGGAAGTGGACTGTGGTCTGGACAAGTCCAATTGTGG
CAAACTGCAGAACCACACATTGGACTACAATCTAGCCCCGGGACTCCTAGGACGGGGCAGCATGCCTGGG
AAGTATCCTCACAGTGACAAGAGCTTAGGAGAGAAGGTGCCACTTCGGTTACACAGTGAGAAGCCAGAGT
GTCGAATATCAGCCATTTGCTCTCCCAGGGACTCTATGTACCAATCAGTGTGTTTGATATCAGAAGAGAG
GAACGAGTGTGTGATTGCCACAGAGGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212151 representing NM_019454
Red=Cloning site Green=Tags(s)

MTPASRSACRWALLLLAVLWPQQRAAGSGIFQLRLQEFVNQRGMLANGQSCEPGCRTFFRICLKHFQATF
SEGPCTFGNVSTPVLGTNSFVVRDKNSGSGRNPLQLPFNFTWPGTFSLNIQAWHTPGDDLRPETSPGNSL
ISQIIIQGSLAVGKIWRTDEQNDTLTRLSYSYRVICSDNYYGESCSRLCKKRDDHFGHYECQPDGSLSCL
PGWTGKYCDQPICLSGCHEQNGYCSKPDECICRPGWQGRLCNECIPHNGCRHGTCSIPWQCACDEGWGGL
FCDQDLNYCTHHSPCKNGSTCSNSGPKGYTCTCLPGYTGEHCELGLSKCASNPCRNGGSCKDQENSYHCL
CPPGYYGQHCEHSTLTCADSPCFNGGSCRERNQGSSYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLN
RGPSRTCRCRPGFTGTHCELHISDCARSPCAHGGTCHDLENGPVCTCPAGFSGRRCEVRITHDACASGPC
FNGATCYTGLSPNNFVCNCPYGFVGSRCEFPVGLPPSFPWVAVSLGVGLVVLLVLLVMVVVAVRQLRLRR
PDDESREAMNNLSDFQKDNLIPAAQLKNTNQKKELEVDCGLDKSNCGKLQNHTLDYNLAPGLLGRGSMPG
KYPHSDKSLGEKVPLRLHSEKPECRISAICSPRDSMYQSVCLISEERNECVIATEV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019454
ORF Size 2058 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019454.2, NP_062327.1
RefSeq Size 3426 bp
RefSeq ORF 2061 bp
Locus ID 54485
UniProt ID Q9JI71
Cytogenetics 2 E5
MW 75.4 kDa
Summary Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (By similarity). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (PubMed:22323600). During spinal cord neurogenesis, inhibits V2a interneuron fate (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_019454" in other vectors (3)
SKU Description Size Price
MC204452 Dll4 (untagged) - Mouse delta-like 4 (Drosophila) (Dll4), (10ug) 10 ug
$943.00
MR212151L3 Lenti ORF clone of Dll4 (Myc-DDK-tagged) - Mouse delta-like 4 (Drosophila) (Dll4) 10 ug
$928.00
MR212151L4 Lenti ORF clone of Dll4 (mGFP-tagged) - Mouse delta-like 4 (Drosophila) (Dll4) 10 ug
$928.00

Plasmid Map for MR212151

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212151
Circular map for MR212151

file-alt Citations

EHD2 modulates Dll4 endocytosis during blood vessel development
Webb, Francis, Judson et al
Microcirculation (2021)
Cited by 10
AI Summary: OriGene products EHD2 (MR220542) and Dll4 (MR212151) were used in this study to investigate the role of EHD2 in regulating Dll4 endocytosis and Notch signaling during blood vessel development.
Snippet: Objective Despite the absolute requirement of Delta/Notch signaling to activate lateral inhibition during early blood vessel development, many mechanisms remain unclear about how this system is regulated. Our objective was to determine the involvement of Epsin Save;EHD2 was purchased from Origene (MR220542). Dll4 was purchased from Origene (MR212151)
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products