Dnmt1 (NM_010066) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR212056
Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2
$2,078.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dnmt1 |
| Synonyms | Cxxc9; Dnmt; Dnmt1o; m.MmuI; MCMT; Met-1; Met1; MommeD; MommeD2; MTa; MTase |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_010066 |
| ORF Size | 4860 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_010066.1 |
| RefSeq Size | 5367 bp |
| RefSeq ORF | 4860 bp |
| Locus ID | 13433 |
| UniProt ID | P13864 |
| Cytogenetics | 9 7.66 cM |
| MW | 183.6 kDa |
| Summary | This gene encodes a methyltransferase that preferentially methylates cytosines of CpG residues in hemimethylated DNA to generate fully methylated CpG base pairs during DNA replication. This enzyme plays roles in diverse cellular processes including cell cycle regulation, DNA repair, and telomere maintenance. The encoded protein is composed of an N-terminal domain with a nuclear localization sequence and replication fork-targeting domain, a DNA-binding CXXC domain, two bromo-adjacent homology domains, and a C-terminal catalytic domain. Mouse embryonic stem cells mutant for this gene are viable, but when introduced into the germ line, cause a recessive lethal phenotype with mutant embryos displaying stunted growth and developmental defects. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_010066" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC224751 | Dnmt1 (untagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2, (10ug) | 10 ug |
$2,217.00
|
|
| MG212056 | Dnmt1 (tGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1) | 10 ug |
$2,278.00
|
|
| MR212056L3 | Lenti ORF clone of Dnmt1 (Myc-DDK-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2 | 10 ug |
$2,378.00
|
|
| MR212056L4 | Lenti ORF clone of Dnmt1 (mGFP-tagged) - Mouse DNA methyltransferase (cytosine-5) 1 (Dnmt1), transcript variant 2 | 10 ug |
$2,378.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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