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Sall1 (NM_021390) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211923
Sall1 (Myc-DDK-tagged) - Mouse sal-like 1 (Drosophila) (Sall1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,152.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sall1
Synonyms Msal-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211923 representing NM_021390
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCGGAGGAAGCAAGCGAAGCCTCAACATTTCCAATCCGACCCCGAAGTGGCCTCGCTCCCCCGGC
GAGATGGGGACACAGAGAAGGGTCAACCAAGTCGCCCCACTAAGAGCAAGGATGCCCATGTCTGTGGCCG
GTGCTGCGCTGAATTCTTTGAATTATCAGACCTTCTGCTCCATAAAAAGAGCTGCACTAAGAATCAATTA
GTTTTGATTGTCAATGAAAGTCCAGCCTCCCCAGCAAAAGCCTTCCCTCCTGGCCCGTCTCTCAATGATC
CCGACGACCAAATGAAAGACGCGGCGAATAAAGCAGACCAAGAAGACTGCAGCGACCTTTCAGAACCCAA
GGGGCTTGACAGGGAAGAGTCCATGGAGGTGGAGGTCCCAGTTGCCACCACCACCACCACCACCACTGGC
GGCAGCGGCGGGAGTGGTGGCAGCACTCTGAGCGGTGTCACCAACATCACCACCCCGAGCTGCCACAGCG
GCTCTTCCACGGGTACCTCAGCGATCACAACCTCTCTACCTCAACTGGGGGACCTGACGACACTGGGCAA
CTTCTCGGTGATCAACAGCAACGTCATCATTGAGAATCTCCAGAGCACCAAGGTAGCGGTGGCCCAGTTC
TCCCAGGAGGCGAGGTGTGGGGGGGCCTCCGGAGGCAAGCTGCTCATCTCAACCCTCATGGAACAACTCC
TGGCTCTGCAGCAGCAGCAGATTCACCAGCTGCAGCTCATAGAGCAAATACGTCACCAAATACTGCTGCT
GGCCTCTCAGAGCGCAGACCTGCCGGCAGCACCCTCTATCCCGTCTCAAGGTACTTTACGAACATCTGCC
AACCCCTTGACCACACTGAGCTCCCATCTGTCTCAACAGCTGGCGGTGGCAGCTGGATTAGCACAGAGCC
TTGCTAGCCAATCTGCCAACATCAGCGGTGTGAAGCAGCTCCCCCACGTCCAGCTACCTCAGAGCAGCTC
CGGCACCTCCATCGTCCCGCCCAGTGGTGGCACTTCTCCCAACATGAGCATAGTGACGGCTGCAGTGCCC
ACCCCGTCCTCAGAAAAAGTGGCTTCCAATGCTGGTGCCTCCCATGTCAGCTCCCCGGCCGTCTCAGCAT
CGTCCTCACCAGCATTCGCAATAAGCAGTCTGTTGAGTCCTGCATCTAACCCACTTCTACCTCAGCCGAC
CCCAGCTAACGCTGTTTTCCCTACCCCTTTGCCTAACATTGCAACAACCGCAGAAGATTTGAACTCCCTG
TCTGCCTTGGCCCAGCAAAGAAAAAGCAAGCCACCAAATGTCACTGCCTTTGAAGCGAAAAGTACTTCAG
ACGAGGCGTTCTTTAAACACAAGTGCAGGTTCTGTGCGAAGGTCTTCGGGAGCGACAGTGCCTTGCAGAT
CCACTTGCGCTCCCATACTGGAGAGAGGCCATTCAAGTGCAACATCTGTGGGAACAGGTTCTCTACTAAG
GGCAATCTGAAAGTCCACTTCCAGCGCCACAAAGAGAAGTACCCCCACATCCAGATGAACCCCTACCCTG
TACCGGAGCACTTGGACAATGTCCCCACGAGCACCGGCATCCCCTACGGCATGTCCATCCCTCCAGAGAA
GCCAGTTACCAGCTGGCTAGACACCAAGCCAGTCCTGCCCACTCTGACCACTTCCGTCGGCCTACCATTG
CCTCCAACTCTCCCAAGCCTCACGCCCTTCATCAAGACGGAAGAGCCAGCCCCCATCCCTATTAGCCATT
CTGCTGCCAGTCCCCAAGGCTCAGTCAAAAGTGACTCCGGGGCCCCTGATTTGGCCACAAGAAACCCAAG
TGGCGTCCCTGAGGAAGTGGAAGGGTCTGCTGTGCCACCCTTTGGTGGCAAGGGTGAAGAGAGTAACATG
GCAAGCTCCGCCGTCCCGACAGCGGGCAACAGTACTCTGAACTCCCCAGTGGCTGACGGTGGTCCAGGAG
GGACCACCTTCACCAACCCTCTGTTGCCCCTCATGTCTGAGCAGTTCAAGGCCAAGTTTCCTTTTGGGGG
ACTCTTAGATTCGGCCCAGGCCTCAGAGACATCCAAGCTGCAGCAACTGGTAGAAAACATTGACAAGAAG
GCCACGGATCCCAATGAGTGTATCATCTGCCACCGGGTTCTCAGCTGTCAGAGCGCCTTGAAAATGCACT
ACCGGACACACACTGGGGAGAGGCCCTTCAAGTGTAAGATCTGCGGCCGGGCTTTCACCACGAAAGGGAA
CCTCAAGACCCACTACAGCGTCCATCGGGCTATGCCTCCACTCAGAGTCCAGCATTCCTGCCCCATCTGT
CAGAAGAAGTTCACAAACGCAGTGGTCCTCCAGCAGCATATTCGTATGCACATGGGAGGCCAGATCCCCA
ACACCCCAGTCCCTGACAACTACCCTGAGTCCATGGAGTCTGACACAGGCTCCTTTGATGAGAAAAATTT
CGACGACTTAGACAACTTCTCAGATGAGAATATGGAAGAGTGTCCTGAGGGCAGCATCCCAGACACACCC
AAGTCAGCGGACGCTTCCCAAGATAGCCTGTCATCTTCGCCTCTGCCCCTCGAGATGTCGAGCATCGCTG
CTCTGGAAAATCAGATGAAGATGATCAATGCTGGCCTGGCGGAGCAGCTGCAGGCCAGCCTGAAGTCTGT
GGAGAACGGATCCATGGAAGGGGATGTGCTGACCAACGACTCTTCCTCAGTGGGTGGCGACATGGAGAGC
CAGAGTGCAGGCAGCCCAGCCATCTCAGAGTCTACCTCTTCCATGCAGGCTCTGTCCCCGTCCAACAGCA
CCCAAGAATTCCACAAGTCACCCGGCATGGAGGAAAAGCCACAGCGAGTGGGGCCAGGCGAGTTCGCCAA
TGGTCTATCTCCCACCCCAGTGAACGGAGGTGCTTTGGACTTGACTTCTAGTCATGCAGAGAAAATCATC
AAAGAAGATTCTCTGGGAATCCTCTTTCCTTTCAGAGACCGGGGTAAATTTAAAAACACTGCTTGCGACA
TTTGTGGCAAGACCTTTGCTTGTCAGAGTGCCTTGGACATTCACTACAGAAGTCATACCAAAGAGAGACC
GTTCATTTGCACAGTTTGCAATCGTGGCTTTTCCACAAAGGGTAATTTGAAGCAGCACATGTTGACACAT
CAGATGCGAGATCTGCCATCGCAGCTCTTTGAGCCCAGTTCCAACCTCGGCCCCAATCAGAACTCTGCAG
TGATTCCCGCCAACTCGCTGTCATCTCTCATCAAAACAGAGGTCAACGGCTTTGTACACGTTTCTCCTCA
GGACAGTAAGGACGCCCCCACTAGTCATGTCCCTCAGGGGCCTCTGTCGTCCTCTGCGACATCCCCAGTT
CTGCTCCCAGCTCTGCCCCGGAGAACTCCCAAACAGCACTATTGTAACACGTGTGGTAAAACCTTCTCTT
CTTCGAGTGCCCTGCAGATCCACGAGAGAACTCACACTGGAGAGAAGCCCTTTGCTTGCACTATCTGTGG
AAGAGCATTCACAACAAAAGGCAATCTGAAGGTCCACATGGGCACCCATATGTGGAACAGCACCCCTGCA
CGCCGGGGCCGGCGGCTCTCAGTGGATGGCCCCATGACATTTCTAGGAGGCAATCCTGTCAAGTTCCCAG
AAATGTTCCAGAAGGATCTAGCGGCGAGGTCAGGAAGTGGGGATCCGTCCAGCTTTTGGAACCAGTATAC
AGCAGCACTGTCCAACGGGCTGGCCATGAAGGCCAACGAGATCTCTGTCATTCAGAACGGCGGCATCCCT
CCAATTCCTGGAAGCCTGGGCAGTGGGAGCAGCTCACCTATCAGTGGGCTGACTGGGAACGTGGAAAAAC
TGGGCAATTCGGAACCCAGTGCTCCTCTGGCTGGCCTGGAGAAAATGGCGAGCAGCGAGAACGGAACCAA
CTTCCGTTTCACCCGCTTCGTGGAGGACAGCAAAGAGATCGTCACAAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211923 representing NM_021390
Red=Cloning site Green=Tags(s)

MSRRKQAKPQHFQSDPEVASLPRRDGDTEKGQPSRPTKSKDAHVCGRCCAEFFELSDLLLHKKSCTKNQL
VLIVNESPASPAKAFPPGPSLNDPDDQMKDAANKADQEDCSDLSEPKGLDREESMEVEVPVATTTTTTTG
GSGGSGGSTLSGVTNITTPSCHSGSSTGTSAITTSLPQLGDLTTLGNFSVINSNVIIENLQSTKVAVAQF
SQEARCGGASGGKLLISTLMEQLLALQQQQIHQLQLIEQIRHQILLLASQSADLPAAPSIPSQGTLRTSA
NPLTTLSSHLSQQLAVAAGLAQSLASQSANISGVKQLPHVQLPQSSSGTSIVPPSGGTSPNMSIVTAAVP
TPSSEKVASNAGASHVSSPAVSASSSPAFAISSLLSPASNPLLPQPTPANAVFPTPLPNIATTAEDLNSL
SALAQQRKSKPPNVTAFEAKSTSDEAFFKHKCRFCAKVFGSDSALQIHLRSHTGERPFKCNICGNRFSTK
GNLKVHFQRHKEKYPHIQMNPYPVPEHLDNVPTSTGIPYGMSIPPEKPVTSWLDTKPVLPTLTTSVGLPL
PPTLPSLTPFIKTEEPAPIPISHSAASPQGSVKSDSGAPDLATRNPSGVPEEVEGSAVPPFGGKGEESNM
ASSAVPTAGNSTLNSPVADGGPGGTTFTNPLLPLMSEQFKAKFPFGGLLDSAQASETSKLQQLVENIDKK
ATDPNECIICHRVLSCQSALKMHYRTHTGERPFKCKICGRAFTTKGNLKTHYSVHRAMPPLRVQHSCPIC
QKKFTNAVVLQQHIRMHMGGQIPNTPVPDNYPESMESDTGSFDEKNFDDLDNFSDENMEECPEGSIPDTP
KSADASQDSLSSSPLPLEMSSIAALENQMKMINAGLAEQLQASLKSVENGSMEGDVLTNDSSSVGGDMES
QSAGSPAISESTSSMQALSPSNSTQEFHKSPGMEEKPQRVGPGEFANGLSPTPVNGGALDLTSSHAEKII
KEDSLGILFPFRDRGKFKNTACDICGKTFACQSALDIHYRSHTKERPFICTVCNRGFSTKGNLKQHMLTH
QMRDLPSQLFEPSSNLGPNQNSAVIPANSLSSLIKTEVNGFVHVSPQDSKDAPTSHVPQGPLSSSATSPV
LLPALPRRTPKQHYCNTCGKTFSSSSALQIHERTHTGEKPFACTICGRAFTTKGNLKVHMGTHMWNSTPA
RRGRRLSVDGPMTFLGGNPVKFPEMFQKDLAARSGSGDPSSFWNQYTAALSNGLAMKANEISVIQNGGIP
PIPGSLGSGSSSPISGLTGNVEKLGNSEPSAPLAGLEKMASSENGTNFRFTRFVEDSKEIVTS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021390
ORF Size 3969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021390.3, NP_067365.2
RefSeq Size 5275 bp
RefSeq ORF 3972 bp
Locus ID 58198
UniProt ID Q6P5E3
Cytogenetics 8 C3
MW 140.7 kDa
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Documents
Resources
"NM_021390" in other vectors (4)
SKU Description Size Price
MC203471 Sall1 (untagged) - Mouse sal-like 1 (Drosophila) (Sall1), (10ug) 10 ug
$1,152.00
MG211923 Sall1 (tGFP-tagged) - Mouse sal-like 1 (Drosophila) (Sall1) 10 ug
$1,352.00
MR211923L3 Lenti ORF clone of Sall1 (Myc-DDK-tagged) - Mouse sal-like 1 (Drosophila) (Sall1) 10 ug
$1,452.00
MR211923L4 Lenti ORF clone of Sall1 (mGFP-tagged) - Mouse sal-like 1 (Drosophila) (Sall1) 10 ug
$1,452.00

Plasmid Map for MR211923

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211923
Circular map for MR211923

file-alt Citations

SALL1 regulates commitment of odontoblast lineages by interacting with RUNX2 to remodel open chromatin regions
Lin, Xiao, Lin et al
Stem Cells (2020)
Cited by 29
AI Summary: OriGene's Flag-SALL1 plasmid (MR211923) was used in co-immunoprecipitation assays to investigate the interaction between SALL1 and RUNX2 in this study.
Snippet: MUT- pGL3-Runx2 and MUT-pGL3-Tgf-β2 constructs. For the co-immunoprecipitation assay, we purchased the Flag- SALL1 plasmid (MR211923, Origene, Rockville, MD, USA). The KOD- Plus-Mutagenesis Kit was used to
Generation of metabolically functional hepatocyte-like cells from dedifferentiated fat cells by Foxa2, Hnf4a, and Sall1 transduction
Hagiwara, Oki, Matsumaru et al
Genes Cells (2020)
Cited by 5
AI Summary: OriGene plasmids MR227662 (Foxa2), MR227354 (Hnf4a), and MC203471 (Sall1) were used as sources of mouse cDNAs for retroviral transduction to generate hepatocyte-like cells from dedifferentiated fat cells in this study.
Snippet: … All rights reserved 4.8 Retroviral gene transfer Mouse cDNAs for full-length Foxa2, Hnf4a, and Sall1 were obtained by digestion of ORIGENE plasmids (MR227662, MR227354, and MC203471) with NotI and BamHI, and the resulting;- This article is protected by copyright. All rights reserved 4.8 Retroviral gene transfer Mouse cDNAs for full-length Foxa2, Hnf4a, and Sall1 were obtained by digestion of ORIGENE plasmids (MR227662, MR227354, and MC203471) with NotI and BamHI, and the resulting
Showing 1-2 of 2 papers.
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