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Aebp1 (NM_009636) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211674
Aebp1 (Myc-DDK-tagged) - Mouse AE binding protein 1 (Aebp1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $983.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aebp1
Synonyms ACLP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211674 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCAGTGCGCACCGCATCCCTGCTCTGCGGCCTCCTGGCACTGCTGACGCTGTGCCCTGAGGGGA
ACCCACAGACGGTGCTGACGGACGACGAGATCGAGGAGTTCCTCGAAGGCTTCCTTTCGGAGTTGGAGAC
CCAGTCCCCGCCCCGGGAAGACGACGTGGAAGTCCAGCCGCTTCCCGAACCCACCCAGCGTCCCCGCAAA
TCCAAGGCAGGGGGCAAGCAGCGGGCAGATGTAGAAGTCCCTCCAGAAAAAAACAAAGACAAAGAGAAGA
AAGGAAAGAAGGACAAAGGCCCCAAAGCCACAAAACCCCTGGAGGGCTCTACCAGGCCCACCAAGAAACC
AAAGGAGAAGCCACCCAAGGCCACCAAGAAGCCCAAGGAGAAACCACCCAAGGCCACCAAGAAGCCCAAG
GAGAAGCCACCCAAGGCCACCAAGAAGCCTAAGGAGAAGCCACCCAAGGCCACTAAGAGGCCCTCGGCAG
GAAAGAAGTTCTCAACTGTGGCCCCCTTGGAAACGCTGGATCGGTTACTCCCCTCACCCTCCAACCCCAG
CGCCCAGGAGCTACCGCAGAAGAGAGACACACCCTTCCCAAATGCCTGGCAAGGTCAAGGAGAAGAGACC
CAGGTGGAGGCCAAGCAGCCCCGGCCAGAGCCAGAGGAGGAGACTGAGATGCCCACACTGGACTACAATG
ACCAGATAGAGAAGGAGGATTACGAGGATTTTGAGTACATCCGTCGCCAGAAGCAGCCCAGGCCAACACC
CAGCAGGAGGAGGCTCTGGCCAGAGCGCCCTGAGGAGAAGACTGAAGAGCCAGAGGAAAGGAAGGAAGTC
GAGCCACCTCTGAAGCCCCTGCTGCCTCCGGACTATGGGGATAGCTACGTGATCCCCAACTATGATGACT
TGGACTATTATTTCCCCCACCCTCCACCGCAGAAGCCTGATGTTGGACAAGAGGTGGATGAGGAAAAGGA
AGAGATGAAGAAGCCCAAAAAGGAGGGTAGTAGCCCCAAGGAGGACACAGAGGACAAGTGGACCGTGGAG
AAAAACAAGGACCACAAAGGGCCCCGGAAGGGTGAGGAGCTGGAGGAGGAGTGGGCGCCAGTGGAGAAAA
TCAAGTGCCCACCTATTGGGATGGAGTCACACCGCATTGAGGACAACCAGATCCGTGCCTCCTCCATGCT
GCGCCACGGCCTCGGAGCCCAGCGGGGCCGGCTCAACATGCAGGCTGGTGCCAATGAAGATGACTACTAT
GACGGGGCATGGTGTGCTGAGGACGAGTCGCAGACCCAGTGGATCGAGGTGGACACCCGAAGGACAACTC
GGTTCACGGGCGTCATCACTCAGGGCCGTGACTCCAGCATCCATGACGACTTCGTGACTACCTTCTTTGT
GGGCTTCAGCAATGACAGCCAGACCTGGGTGATGTACACCAATGGCTACGAGGAAATGACCTTCTATGGA
AATGTGGACAAGGACACACCTGTGCTGAGCGAGCTCCCTGAGCCAGTTGTGGCCCGTTTCATCCGCATCT
ATCCACTCACCTGGAACGGTAGCCTGTGCATGCGCCTGGAGGTGCTAGGCTGCCCCGTGACCCCTGTCTA
CAGCTACTACGCACAGAATGAGGTGGTAACTACTGACAGCCTGGACTTCCGGCACCACAGCTACAAGGAC
ATGCGCCAGCTGATGAAGGCTGTCAATGAGGAGTGCCCCACAATCACTCGCACATACAGCCTGGGCAAGA
GTTCACGAGGGCTCAAGATCTACGCAATGGAAATCTCAGACAACCCTGGGGATCATGAACTGGGGGAGCC
CGAGTTCCGCTACACAGCCGGGATCCACGGCAATGAGGTGCTAGGCCGAGAGCTCCTGCTCCTGCTCATG
CAATACCTATGCCAGGAGTACCGCGATGGGAACCCGAGAGTGCGCAACCTGGTGCAGGACACACGCATCC
ACCTGGTGCCCTCGCTGAACCCTGATGGCTATGAGGTGGCAGCGCAGATGGGCTCAGAGTTTGGGAACTG
GGCACTGGGGCTGTGGACTGAGGAGGGCTTTGACATCTTCGAGGACTTCCCAGATCTCAACTCTGTGCTC
TGGGCAGCTGAGGAGAAGAAATGGGTCCCCTACAGGGTCCCAAACAATAACTTGCCAATCCCTGAACGTT
ACCTGTCCCCAGATGCCACGGTCTCCACAGAAGTCCGGGCCATTATTTCCTGGATGGAGAAGAACCCCTT
TGTGCTGGGTGCAAATCTGAACGGTGGTGAGCGGCTTGTGTCTTATCCCTATGACATGGCCCGGACACCT
AGCCAGGAGCAGCTGTTGGCCGAGGCACTGGCAGCTGCCCGCGGAGAAGATGATGACGGGGTGTCTGAGG
CCCAGGAGACTCCAGATCACGCTATTTTCCGCTGGCTGGCCATCTCATTTGCCTCCGCCCATCTCACCAT
GACGGAGCCCTACCGGGGAGGGTGCCAGGCCCAGGACTACACCAGCGGCATGGGCATTGTCAACGGGGCC
AAGTGGAATCCTCGCTCTGGGACTTTCAATGACTTTAGCTACCTGCACACAAACTGTCTGGAGCTCTCCG
TATACCTGGGCTGTGACAAGTTCCCCCACGAGAGTGAGCTACCCCGAGAATGGGAGAACAACAAAGAAGC
GCTGCTCACCTTCATGGAGCAGGTGCACCGTGGCATTAAGGGTGTGGTGACAGATGAGCAAGGCATCCCC
ATTGCCAATGCCACCATCTCTGTGAGTGGCATCAACCATGGTGTGAAGACAGCAAGTGGAGGTGACTACT
GGCGCATTCTGAACCCGGGTGAGTACCGTGTGACAGCTCACGCAGAGGGCTACACCTCAAGTGCCAAGAT
CTGCAATGTGGACTACGATATTGGGGCCACTCAGTGCAACTTCATCCTGGCTCGATCCAACTGGAAGCGC
ATTCGGGAGATCTTGGCTATGAACGGGAACCGTCCCATTCTCCGAGTTGACCCCTCACGACCCATGACCC
CCCAGCAGCGGCGCATGCAGCAGCGCCGTCTACAGTACCGGCTCCGCATGAGGGAACAGATGCGACTGCG
TCGCCTCAATTCTACCGCAGGCCCTGCCACAAGCCCCACTCCTGCCCTTATGCCTCCCCCTTCCCCTACA
CCAGCCATTACCTTGAGGCCCTGGGAAGTTCTACCCACTACCACTGCAGGCTGGGAGGAGTCAGAGACTG
AGACCTATACAGAAGTAGTGACAGAGTTTGAGACAGAGTATGGGACTGACCTAGAGGTGGAAGAGATAGA
GGAGGAGGAGGAGGAGGAGGAGGAAGAGATGGACACAGGCCTTACATTTCCACTCACAACAGTGGAGACC
TACACAGTGAACTTTGGGGACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211674 protein sequence
Red=Cloning site Green=Tags(s)

MAPVRTASLLCGLLALLTLCPEGNPQTVLTDDEIEEFLEGFLSELETQSPPREDDVEVQPLPEPTQRPRK
SKAGGKQRADVEVPPEKNKDKEKKGKKDKGPKATKPLEGSTRPTKKPKEKPPKATKKPKEKPPKATKKPK
EKPPKATKKPKEKPPKATKRPSAGKKFSTVAPLETLDRLLPSPSNPSAQELPQKRDTPFPNAWQGQGEET
QVEAKQPRPEPEEETEMPTLDYNDQIEKEDYEDFEYIRRQKQPRPTPSRRRLWPERPEEKTEEPEERKEV
EPPLKPLLPPDYGDSYVIPNYDDLDYYFPHPPPQKPDVGQEVDEEKEEMKKPKKEGSSPKEDTEDKWTVE
KNKDHKGPRKGEELEEEWAPVEKIKCPPIGMESHRIEDNQIRASSMLRHGLGAQRGRLNMQAGANEDDYY
DGAWCAEDESQTQWIEVDTRRTTRFTGVITQGRDSSIHDDFVTTFFVGFSNDSQTWVMYTNGYEEMTFYG
NVDKDTPVLSELPEPVVARFIRIYPLTWNGSLCMRLEVLGCPVTPVYSYYAQNEVVTTDSLDFRHHSYKD
MRQLMKAVNEECPTITRTYSLGKSSRGLKIYAMEISDNPGDHELGEPEFRYTAGIHGNEVLGRELLLLLM
QYLCQEYRDGNPRVRNLVQDTRIHLVPSLNPDGYEVAAQMGSEFGNWALGLWTEEGFDIFEDFPDLNSVL
WAAEEKKWVPYRVPNNNLPIPERYLSPDATVSTEVRAIISWMEKNPFVLGANLNGGERLVSYPYDMARTP
SQEQLLAEALAAARGEDDDGVSEAQETPDHAIFRWLAISFASAHLTMTEPYRGGCQAQDYTSGMGIVNGA
KWNPRSGTFNDFSYLHTNCLELSVYLGCDKFPHESELPREWENNKEALLTFMEQVHRGIKGVVTDEQGIP
IANATISVSGINHGVKTASGGDYWRILNPGEYRVTAHAEGYTSSAKICNVDYDIGATQCNFILARSNWKR
IREILAMNGNRPILRVDPSRPMTPQQRRMQQRRLQYRLRMREQMRLRRLNSTAGPATSPTPALMPPPSPT
PAITLRPWEVLPTTTAGWEESETETYTEVVTEFETEYGTDLEVEEIEEEEEEEEEEMDTGLTFPLTTVET
YTVNFGDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009636
ORF Size 3384 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009636.3
RefSeq Size 4049 bp
RefSeq ORF 3387 bp
Locus ID 11568
UniProt ID Q640N1
Cytogenetics 11 A1
MW 128.4 kDa
Summary Isoform 1: As a positive regulator of collagen fibrillogenesis, it is probably involved in the organization and remodeling of the extracellualr matrix.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009636" in other vectors (3)
SKU Description Size Price
MG211674 Aebp1 (tGFP-tagged) - Mouse AE binding protein 1 (Aebp1) 10 ug
$789.00 $1,183.00
MR211674L3 Lenti ORF clone of Aebp1 (Myc-DDK-tagged) - Mouse AE binding protein 1 (Aebp1) 10 ug
$1,283.00
MR211674L4 Lenti ORF clone of Aebp1 (mGFP-tagged) - Mouse AE binding protein 1 (Aebp1) 10 ug
$1,283.00

Plasmid Map for MR211674

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211674
Circular map for MR211674

file-alt Citations

Aortic carboxypeptidase-like protein, a WNT ligand, exacerbates nonalcoholic steatohepatitis
Teratani, Tomita, Suzuki et al
J. Clin. Invest. (2018)
AI Summary: In this study, the Mouse ACLP-DDK expression vector from OriGene Technologies was used to investigate the role of ACLP in activating canonical WNT signaling and exacerbating nonalcoholic steatohepatitis (NASH).
Snippet: thcare. Anti-His tag antibody was purchased from Medical & Biological Laboratories. See Supplemental Table 1 for information about the antibodies. Mouse ACLP-DDK expression vector was purchased from OriGene Technologies. TOPflash and FOPflash luciferase reporters and expression vectors for human LRP6, human LRP6N-IgG, mouse FZD8, mouse FZD8CRD-IgG, and mouse WNT3A were purchased from Addgene. In
Showing 1-1 of 1 papers.
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