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Hdac5 (NM_010412) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211655
Hdac5 (Myc-DDK-tagged) - Mouse histone deacetylase 5 (Hdac5), transcript variant 2
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$589.00 $970.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hdac5
Synonyms AI426555; Hdac4; mHDA1; mKIAA0600
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211655 representing NM_010412
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACTCTCCCAACGAGTCGGATGGCATGTCAGGCCGGGAACCATCCTTGGAAATCCTGCCACGGACTC
CTCTGCACAGCATCCCTGTGGCAGTGGAGGTGAAGCCGGTGCTGCCAGGAGCCATGCCCAGCTCCATGGG
GGGTGGAGGTGGAGGTAGCCCCAGCCCCGTGGAGCTTCGGGGGGCTCTGGCGGGCCCCATGGACCCTGCG
CTACGGGAGCAGCAACTGCAGCAGGAGCTCCTGGTCCTCAAGCAGCAGCAGCAGCTCCAGAAGCAGCTCC
TGTTCGCCGAGTTCCAGAAGCAGCACGACCACTTGACGCGGCAGCACGAGGTCCAGCTGCAGAAGCACCT
CAAGCAGCAGCAGGAGATGCTGGCGGCTAAGAGGCAGCAGGAGCTGGAGCAGCAGCGGCAGCGGGAGCAG
CAGCGGCAGGAGGAGCTGGAGAAACAGCGGCTGGAGCAGCAGCTGCTCATCCTGCGCAACAAGGAGAAGA
GCAAAGAGAGTGCCATCGCCAGCACCGAGGTAAAGCTGAGGCTCCAGGAATTCCTGTTGTCCAAGTCAAA
GGAGCCCACGCCAGGCGGCCTCAACCATTCCCTCCCACAGCACCCCAAATGCTGGGGAGCCCACCACGCT
TCTTTGGACCAGAGTTCCCCTCCCCAGAGCGGCCCTCCTGGGACGCCTCCCTCCTACAAATTGCCTTTGC
TTGGGCCCTATGACAGCCGTGATGACTTTCCCCTCCGTAAAACGGCCTCGGAACCCAACTTAAAAGTACG
TTCGAGGCTAAAACAGAAGGTAGCCGAGAGGAGAAGCAGTCCCCTCCTGCGTCGAAAGGATGGCACTGTT
ATTAGTACTTTTAAGAAGAGAGCAGTTGAGATCACCGGCACGGGGCCTGGGGTGTCGTCCGTGTGTAACA
GTGCGCCCGGCTCTGGCCCCAGCTCTCCCAACAGTTCCCACAGCACCATCGCTGAGAACGGCTTTACTGG
CTCAGTCCCCAACATCCCCACTGAGATGATCCCCCAGCACCGGGCCCTCCCTCTGGACAGTTCCCCAAAC
CAGTTCAGCCTCTATACGTCTCCTTCTCTGCCCAACATCTCCCTAGGGCTGCAGGCCACTGTCACTGTCA
CCAACTCGCACCTCACCGCCTCCCCGAAGCTGTCAACACAGCAGGAGGCTGAGAGGCAGGCCCTTCAGTC
CCTGCGGCAGGGCGGCACACTGACCGGCAAGTTCATGAGCACATCCTCCATCCCTGGCTGCCTGTTGGGA
GTGGCACTGGAGGGTGACACAAGCCCCCACGGGCACGCTTCCCTGCTGCAGCACGTTTTGCTCCTGGAGC
AGGCCCGGCAACAGAGCACGCTCATAGCAGTGCCGCTCCATGGGCAGTCCCCACTGGTGACGGGTGAACG
TGTGGCCACCAGCATGAGGACGGTGGGTAAGCTCCCGAGGCACCGACCTCTGAGCCGCACTCAGTCCTCC
CCGCTGCCGCAGAGTCCCCAGGCCCTGCAGCAGCTGGTCATGCAGCAGCAGCACCAGCAGTTCCTGGAGA
AGCAGAAGCAGCAGCAGATGCAGCTGGGCAAGATCCTTACCAAAACTGGGGAGCTGTCAAGGCAGCCCAC
CACTCACCCGGAGGAGACAGAAGAGGAGCTGACGGAGCAGCAGGAGGCCTTGCTGGGAGAGGGGGCCCTG
ACCATTCCCCGGGAAGGCTCTACAGAAAGTGAGAGCACCCAGGAAGACCTAGAAGAGGAGGAGGAGGAGG
AGGAGGAGGAAGAGGAGGACTGCATTCAGGTCAAGGATGAGGATGGCGAGAGTGGTCCTGATGAAGGCCC
TGACTTAGAAGAGTCCAGTGCTGGTTACAAAAAGTTGTTCGCAGATGCCCAGCAGTTACAGCCCCTCCAG
GTGTACCAGGCACCCCTCAGCCTGGCCACTGTGCCTCATCAGGCCCTGGGCCGCACCCAGTCCTCACCTG
CTGCTCCTGGGAGCATGAAGAGCCCCACAGACCAACCCACTGTGGTGAAGCACCTCTTCACCACAGGTGT
GGTCTATGACACGTTCATGCTGAAGCACCAGTGTATGTGCGGAAACACACACGTGCACCCAGAGCACGCC
GGCCGCATCCAGAGCATCTGGTCCCGGCTGCAGGAAACTGGTCTGCTCGGCAAGTGTGAGCGGATCCGGG
GTCGTAAAGCCACACTGGATGAAATCCAGACCGTGCACTCTGAGTACCACACCCTGCTCTATGGGACCAG
CCCCCTTAACCGGCAGAAGCTGGACAGCAAGAAGCTGCTTGGCCCCATCAGCCAGAAGATGTACGCCATG
CTGCCCTGTGGGGGCATTGGGGTGGACAGTGACACGGTGTGGAATGAGATGCACTCCTCAAGTGCCGTGC
GAATGGCAGTGGGCTGCCTGGTGGAGCTGGCCTTCAAGGTGGCTGCAGGAGAGCTCAAGAATGGATTTGC
TATCATCCGGCCCCCAGGACACCATGCTGAGGAGTCCACAGCCATGGGATTCTGCTTCTTCAACTCCGTA
GCCATCACAGCTAAACTCCTGCAGCAGAAGCTGAGCGTGGGCAAGGTCCTCATCGTGGACTGGGATATTC
ACCATGGCAACGGCACCCAGCAAGCATTCTACAACGATCCCTCTGTGCTCTACATCTCCCTGCATCGCTA
CGACAACGGGAACTTCTTTCCAGGCTCTGGGGCTCCTGAAGAGGTTGGTGGAGGGCCAGGTGTGGGGTAC
AACGTAAATGTGGCGTGGACAGGAGGTGTGGATCCCCCCATTGGAGATGTGGAATACCTGACAGCCTTCA
GGACAGTGGTGATGCCCATTGCCCAGGAGTTCTCACCTGACGTCGTCCTAGTCTCCGCTGGGTTTGATGC
TGTTGAAGGACATCTGTCTCCACTGGGTGGCTATTCTGTCACCGCCAGATGTTTTGGCCACTTGACCAGG
CAGCTCATGACACTGGCTGGGGGCCGGGTGGTGCTGGCCCTGGAGGGAGGCCATGACTTGACCGCCATCT
GTGATGCCTCTGAGGCCTGTGTCTCGGCTCTGCTCAGCGTGGAGCTGCAGCCCTTGGATGAAGCAGTCTT
GCAGCAAAAGCCCAGCGTCAATGCGGTTGCCACACTAGAGAAAGTCATCGAGATCCAGAGCAAACACTGG
AGCTGTGTACAGAGGTTTGCCGCTGGTCTGGGCTGCTCGCTGCGGGAGGCTCAGACAGGTGAGAAAGAGG
AGGCCGAGACTGTGAGCGCCATGGCCCTGCTTTCCGTGGGGGCTGAGCAGGCCCAGGCTGTTGCCACTCA
AGAGCACAGCCCCAGGCCAGCAGAGGAGCCCATGGAGCAGGAGCCTGCCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211655 representing NM_010412
Red=Cloning site Green=Tags(s)

MNSPNESDGMSGREPSLEILPRTPLHSIPVAVEVKPVLPGAMPSSMGGGGGGSPSPVELRGALAGPMDPA
LREQQLQQELLVLKQQQQLQKQLLFAEFQKQHDHLTRQHEVQLQKHLKQQQEMLAAKRQQELEQQRQREQ
QRQEELEKQRLEQQLLILRNKEKSKESAIASTEVKLRLQEFLLSKSKEPTPGGLNHSLPQHPKCWGAHHA
SLDQSSPPQSGPPGTPPSYKLPLLGPYDSRDDFPLRKTASEPNLKVRSRLKQKVAERRSSPLLRRKDGTV
ISTFKKRAVEITGTGPGVSSVCNSAPGSGPSSPNSSHSTIAENGFTGSVPNIPTEMIPQHRALPLDSSPN
QFSLYTSPSLPNISLGLQATVTVTNSHLTASPKLSTQQEAERQALQSLRQGGTLTGKFMSTSSIPGCLLG
VALEGDTSPHGHASLLQHVLLLEQARQQSTLIAVPLHGQSPLVTGERVATSMRTVGKLPRHRPLSRTQSS
PLPQSPQALQQLVMQQQHQQFLEKQKQQQMQLGKILTKTGELSRQPTTHPEETEEELTEQQEALLGEGAL
TIPREGSTESESTQEDLEEEEEEEEEEEEDCIQVKDEDGESGPDEGPDLEESSAGYKKLFADAQQLQPLQ
VYQAPLSLATVPHQALGRTQSSPAAPGSMKSPTDQPTVVKHLFTTGVVYDTFMLKHQCMCGNTHVHPEHA
GRIQSIWSRLQETGLLGKCERIRGRKATLDEIQTVHSEYHTLLYGTSPLNRQKLDSKKLLGPISQKMYAM
LPCGGIGVDSDTVWNEMHSSSAVRMAVGCLVELAFKVAAGELKNGFAIIRPPGHHAEESTAMGFCFFNSV
AITAKLLQQKLSVGKVLIVDWDIHHGNGTQQAFYNDPSVLYISLHRYDNGNFFPGSGAPEEVGGGPGVGY
NVNVAWTGGVDPPIGDVEYLTAFRTVVMPIAQEFSPDVVLVSAGFDAVEGHLSPLGGYSVTARCFGHLTR
QLMTLAGGRVVLALEGGHDLTAICDASEACVSALLSVELQPLDEAVLQQKPSVNAVATLEKVIEIQSKHW
SCVQRFAAGLGCSLREAQTGEKEEAETVSAMALLSVGAEQAQAVATQEHSPRPAEEPMEQEPAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010412
ORF Size 3342 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010412.3, NP_034542.3
RefSeq Size 3813 bp
RefSeq ORF 3345 bp
Locus ID 15184
Cytogenetics 11 D
MW 121.6 kDa
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Documents
Resources

Plasmid Map for MR211655

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211655
Circular map for MR211655

file-alt Citations

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