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Daam1 (NM_026102) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211599
Daam1 (Myc-DDK-tagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $937.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Daam1
Synonyms 1700066F09Rik; 2310028E21Rik; AI503486; E130308H01; mKIAA0666
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211599 representing NM_026102
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCACGGAAGAGAGGCGGCCGGGGGATTTCATTTATCTTTTGCTGTTTCCGAAACAATGACCATC
CAGAAATCACATATCGGCTTAGGAATGATAGCAACTTTGCACTTCAGACAATGGAGCCGGCGCTGCCCAT
GCCCCCTGTGGAGGAGCTCGATGTCATGTTCAGTGAACTTGTGGATGAACTTGACCTCACAGACAAACAC
AGGGAAGCCATGTTTGCACTTCCAGCAGAGAAAAAGTGGCAGATATACTGTAGCAAGAAAAAGGACCAGG
AAGAAAACAAGGGAGCAACCAGTTGGCCCGAGTTCTACATTGATCAGCTCAATTCCATGGCTGCTAGGAA
ATCTCTGCTGGCTTTAGAGAAGGAAGAAGAGGAGGAGAGGAGTAAAACCATCGAGAGTCTGAAGACAGCA
CTGAGAACCAAGCCCATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTGTCATGTATTCTCAACT
TTCTGAAGACCATGGACTATGAGACCTCTGAGTCTCGGATACACACCTCCCTCATTGGCTGTATAAAGGC
ACTAATGAACAACTCTCAAGGCCGGGCCCATGTCCTGGCTCATTCTGAGAGTATCAATGTAATTGCTCAG
AGTCTGAGCACAGAGAACATTAAAACAAAGGTGGCCGTGCTGGAAATCCTGGGCGCTGTGTGCCTGGTTC
CTGGGGGCCACAAGAAAGTTCTGCAAGCTATGCTGCACTACCAGAAGTATGCCAGCGAGAGAACCCGCTT
CCAGACGTTAATTAATGACTTGGATAAAAGCACTGGGCGTTATCGGGATGAAGTAAGTCTCAAGACGGCC
ATCATGTCCTTCATCAATGCAGTGCTGAGCCAGGGAGCCGGAGTGGAGAGTTTGGACTTCAGGCTCCATC
TTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTTATAGATAAATTAAGGGAACATGAAAATTCAAC
ACTAGACAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAATTTGCCAAAAGATTT
GAACTGGTTCACATAGACACAAAAAGCGCCACTCAGATGTTTGAGCTGACCAGGAGGAGGCTAACCCACA
GCGAAGCCTACCCCCACTTCATGTCCATTCTGCACCATTGCCTCCAGATGCCTTACAAGAGGAGTGGTAA
CACAGTTCAGTACTGGCTGCTGTTAGACAGAATCATACAGCAGATAGTTATCCAGAATGACAAAGGGCAG
GATCCCGACTCCACACCTCTGGAAAACTTTAATATCAAGAACGTCGTAAGAATGTTGGTTAATGAAAATG
AAGTTAAGCAATGGAAAGAGCAGGCAGAGAAGATGAGGAAAGAGCACAATGAGCTACAACAGAAGCTGGA
GAAGAAAGAGAGAGAATGTGATGCTAAGACCCAAGAGAAGGAAGAAATGATGCAGACCTTAAACAAGATG
AAAGAGAAGCTGGAAAAGGAGACAACTGAGCACAAGCAAGTTAAGCAGCAGGTGGCAGACCTCACGGCGC
AGCTCCACGAGCTGAACAGGAGGGCCGTCTGTGCAGCAGTCCCAGGAGGACCCTCACCCGGAGCCCCTGG
AGGACCCTTTCCTTCCTCTGGGTTGGGGTCTCTTCTCCCTCCCCCACCACCCCCACTCCTTTCAGGTGGA
GCACTTCCTCCTCCACCACCTCCCCTCCCTCCTGGGGGTCCTCCTCCTCCCCCAGGGCCTCCTCCCTTAG
GAGGAGTTCTGCCACCTCCTGGTGCTCCAGTGAGTCTAACACTCAAGAAGAAGAATATTCCCCAGCCCAC
CAATGCCCTGAAATCCTTCAACTGGTCCAAGTTGCCTGAGAACAAATTGGATGGAACAGTATGGACTGAA
ATTGATGATACCAAAGTCTTCAAAATTCTAGACCTTGAAGACCTAGAAAGAACGTTCTCTGCCTACCAAA
GACAGCAGGAGTTCTTTGTGAACAACTCCAAGCAGAAAGAAGCAGATGCCATTGATGACACACTGAGTTC
CAAACTTAAAGTCAAAGAGCTGTCAGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTGTCG
AGGTTGAAATTATCCAATGATGAAATCAAGCGGGCAATTCTAACAATGGATGAACAGGAGGATCTGCCCA
AGGACATGTTAGAACAGCTTTTGAAATTCGTTCCTGAGAAAAGCGACATCGACCTGCTAGAGGAACACAA
GCATGAGCTGGATCGGATGGCCAAGGCTGACCGCTTCCTGTTTGAGATGAGCAGAATTAATCATTACCAG
CAAAGACTGCAATCACTGTACTTCAAAAAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAGCCCAAAGTAG
AAGCGATTCGCTCTGGCTCTGAGGAGGTGTTCAGGAGCCGCGCCCTCAAGCAGCTCCTGGAGGTGGTCTT
GGCTTTTGGAAATTATATGAATAAAGGACAAAGAGGCAATGCATATGGATTCAAGATCTCCAGCCTCAAC
AAGATAGCGGATACCAAATCCAGTATTGACAAGAACATTACTCTTTTGCACTATCTAATAACTATTGTGG
AGAATAAGTACCCCAAAGTCCTCAACCTAAGCGAAGAGTTGCGGGATATTCCTCAAGCAGCGAAAGTAAA
CATGACTGAGCTGGACAAGGAGATCAGCACCCTGAGGAGTGGCCTGAAAGCTGTAGAGACGGAGCTGGAG
TATCAGAAGTCTCAGCCCCCACAGCCTGGAGACAAGTTCGTGTCTGTTGTCAGCCAGTTCATCACCCTGG
CTAGCTTCAGCTTCTCAGATGTTGAAGATCTTCTAGCAGAAGCTAAAGAGCTGTTCACTAAAGCAGTGAA
GCACTTTGGGGAAGAGGCTGGCAAGATCCAGCCAGATGAGTTCTTCGGGATCTTTGATCAGTTTCTTCAG
GCCGTGGCCGAAGCGAAACAGGAAAATGAGAACATGAGAAAAAGGAAGGAGGAGGAGGAACGGCGGGCTC
GCCTGGAAGCTCAGCTCAAAGAGCAGCGGGAGAGGGAGCGGAAGGTGAGGAAGGCCAAGGAGAGCAGTGA
AGAGAGTGGAGAGTTTGACGACCTGGTCTCGGCGCTGCGCTCAGGAGAGGTGTTCGACAAAGACCTTTCA
AAACTCAAACGGAACCGGAAACGCATTTCCAACCAGGTGACGGACAGCAGCCGGGAGCGACCAATCACAA
AACTTAATTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211599 representing NM_026102
Red=Cloning site Green=Tags(s)

MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKH
REAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTA
LRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQ
SLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTA
IMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRF
ELVHIDTKSATQMFELTRRRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQ
DPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKM
KEKLEKETTEHKQVKQQVADLTAQLHELNRRAVCAAVPGGPSPGAPGGPFPSSGLGSLLPPPPPPLLSGG
ALPPPPPPLPPGGPPPPPGPPPLGGVLPPPGAPVSLTLKKKNIPQPTNALKSFNWSKLPENKLDGTVWTE
IDDTKVFKILDLEDLERTFSAYQRQQEFFVNNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLS
RLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQ
QRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSRALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLN
KIADTKSSIDKNITLLHYLITIVENKYPKVLNLSEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELE
YQKSQPPQPGDKFVSVVSQFITLASFSFSDVEDLLAEAKELFTKAVKHFGEEAGKIQPDEFFGIFDQFLQ
AVAEAKQENENMRKRKEEEERRARLEAQLKEQRERERKVRKAKESSEESGEFDDLVSALRSGEVFDKDLS
KLKRNRKRISNQVTDSSRERPITKLNF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026102
ORF Size 3231 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026102.3, NP_080378.2
RefSeq Size 6004 bp
RefSeq ORF 3234 bp
Locus ID 208846
UniProt ID Q8BPM0
Cytogenetics 12 C3
MW 123.8 kDa
Summary Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments (By similarity). Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (PubMed:26526197).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026102" in other vectors (4)
SKU Description Size Price
MC223437 Daam1 (untagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 1, (10ug) 10 ug
$1,032.00
MG211599 Daam1 (tGFP-tagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 1 10 ug
$1,137.00
MR211599L3 Lenti ORF clone of Daam1 (Myc-DDK-tagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 1 10 ug
$1,237.00
MR211599L4 Lenti ORF clone of Daam1 (mGFP-tagged) - Mouse dishevelled associated activator of morphogenesis 1 (Daam1), transcript variant 1 10 ug
$1,237.00

Plasmid Map for MR211599

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211599
Circular map for MR211599

file-alt Citations

KCTD3 deficiency disrupts axon initial segment organization and neurite outgrowth in a neurodevelopmental disorder mouse model
Oh, Lee, Jin et al
Commun Biol (2026)
AI Summary: In this study, the OriGene mouse Daam1 ORF clone tagged with myc-DDK (FLAG) (catalog number MR211599) was used to overexpress DAAM1 in neurons, enabling the researchers to rescue defects caused by KCTD3 deficiency and investigate its role in neuronal structural organization.
Snippet: TRCN0000413081, identifier NM_172650.2 -779s21c1), all under the control of the U6 promoter. For DAAM1 overexpression, a mouse Daam1 ORF clone tagged with myc -DDK (FLAG) (NM_026102;Origene, MR211599) was subcloned into the FUGW lentiviral vector by replacing the EGFP cassette
Showing 1-1 of 1 papers.
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