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Rnf31 (NM_194346) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211571
Rnf31 (Myc-DDK-tagged) - Mouse ring finger protein 31 (Rnf31)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $929.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf31
Synonyms AL033293; BC031509; Flj10111; HOIP; mFLJ00217; Paul
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211571 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGGGAGACGAGGAGCGAGGCTTCCTGGCGGCCCGCGAGGAGCTGGCGAGCGCCCTGAGGTGGGATT
CTGCGCAGGTTTTTCCCCTGGAGCAGCTCATGCCGCTTCTGGCCACCTCTCTGCCACCAGCCGCCCGCTA
CCTGCAGCTGGACGCCGGACGCTTGGTCCGCTGCAACGCTCATGGGGAGCCTCGAAACTACCTCAACACC
CTATCCACGGCCCTGAACATCCTGGAAAAATATGGTCGCAACCTCCTCAGCCCGCAGCGGCCCCGGTATT
GGCGCTCAGTGAAGTTTAATAACCCCGTCTTTCGCAGCACGGTGGATGCTGTGCAGGGTGGCCGGGATGT
ACTACGGTTGTATGGCTATACTGAGGAGCGCCCAGATGGATTGAGTTTCCCCGAAGGGCAGGAGGAACCA
GATGAATACCAGGTTGCTGTTGTCACACTAGAAGTACTGCTGCTTCGCACCGAGCTCAGTTTGCTGTTGC
AGAATACTCATCCCAGACAGAATGCACTGGACCAGCTGCTAAGAGAGAGCGTTGAAGATGGTATGCTGCA
GCTTTCAGAGTTTCACCCCCTTCTGAGGGAGATTGTTCCTGGCCCCCGCCCCTCTGCCCAAGGCTCCACT
CCTGGTCCCTGTTTCCTCTGTGGTTCTGCCCCAGGCACACTGCACTGTCCAGCCTGTAACCAAGTCTCGT
GCCCAGCTTGTGACATTTTGTTCCATGGGCATCCGTCCCGTGCACATCACCTTCGCCAAGCCCTGCCTGG
GTCCCACCAGACTGCCAGCCTGAGCTCTAGTTTACCTGCCTCGTCCCAACCACGGCCCCCCTCCTCCTCC
TTGGCCCTGGGAGATAGCTCTCTTTCTTCCCCTGACCCTGCAAATGCCTGTCTGCCCTGGCATTGTCTTA
CCTGTGCCACACTAAATGAGCCTTGGGCAGTGTTCTGTGCAGTCTGTAGTCAGCCCAAAGGCTGCAAGGT
GCCGGGAATAGAGGGTTCCCATGGAACCGGGGGCCTAGAACCTGAGCCTGCACGGGATCAATGGGCCTGC
CAGAGCTGTACCTTTGAGAATGAGGCAGCAGCTGTGCTATGCGCCATATGTGAGCGACCTCGGCTGGCCC
AGCCTCCCAGCTTGGTGGTGGATTCCCATGATGCTGGTGTTTGCCAACAGTCCCTTAAGCAGGAGGATCC
TTTGCTCACCGCTGCCCAGCCTCAGGTGTGGTACTGTGACCATTGTACCTTCTGCAATTCAGGCCCTGTC
TGGGTGTGTGCCATGTGCAACCGAACCCGAGACCCCATCCCTACACAGCCTGCCCTCCAGTCCTATCCCA
GCTCTTTGGAAAAGGGACGCCCAAAGCCAGGGTCCTCACAACACCTTGGTTCCTCCCTGCCTGCTTCCTG
TGGAGACCCAGAGAAACAACGCCAAGATAAGATGCGGAAGGAAGGTCTCCAGCTCGTGAGCATGATCCAG
GAAGGAGAAACTGCGGGTGCCAGTCCAGAAGAGGTCTTCTCAGCTCTCCAATACTCAGGCACAGAGGTGC
CCCTCCAGTGGTTGCGTTCAGAGCTGTCCTACGTCCTGGAGATGGTGGCTGAGCTTGCTGGACAACAGGA
TCCAGAGCTGGGGGCCTTTTCCTGTCAGGAAGCCCGGAAAGCCTGGCTTGATCGCCATGGCAACCTGGAT
GAAGCTGTAGAGGAGTGTGTGAGGGCCAGGAGGAGGAAGGTGCACGAGCTGCAGTCCCTGGGCTTTGGGC
CTAAGGAAGGGTCACTACAGGCATTGTTCCAGCATGGGGGTGACGTGGCTCGGGCCCTGACTGAGTTACA
GCGCCAGCGCCTGGAGCCCTTCCATCAGCGCCTATGGGACAGAGACCCTGAACCCACTCCCTGCTGGGAT
GGGCTGGACAGACAGAGCTTGGTCAGACGCCTTCTGGCCGTCTACACACTCCCCAGCTGGGGCCGAGCAG
AGCTGGCGCTGGCGCTGCTGCAGGAGACACCCAGGAACTATGAGTTGTTGGACGTGGTGGAGGCTGTGAG
GCACAGCCAGGACCGGGCCTTTCTGCGTCGACTGCTTGCCCAGGAATGTGCTGTGTGCGGGTGGGCCCTT
CCCCGCAACCGGATGCAGGCCCTGATCTCCTGTGAGTGCACCATATGTCCCGAATGCTTCCGCCAACACT
TCACCATTGCCCTGAAGGAGAAGCACATCACAGACATGGTGTGCCCTGCCTGTGGCCGCCCTGACCTCAC
TGATGACGCTCAGTTACTCAGCTACTTCTCCACCCTTGACATCCAGCTCAGAGAGAGCCTAGACCCCGAT
GCATATGCCCTGTTTCACAAGAAGCTGACCGAGGCTGTGCTTATGCGAGACCCCAAGTTCTTGTGGTGCG
CCCAGTGTTCCTTTGGCTTCATCTATGAACGCGAACAGCTGGAGGCGACGTGTCCCCAGTGTCACCAGAC
CTTCTGTGTGCGCTGCAAGCGCCAGTGGGAGGAGCAGCACAGAGGACGGAGTTGTGAGGATTTCCAGAAC
TGGAAACGCACCAATGACCCAGAGTACCAGGCTCAAGGCTTGGCCATGTACCTTCAGGAAAACGGCATTG
ACTGTCCGAAATGCAAGTTCTCGTACGCACTGGCCCGGGGAGGCTGCATGCACTTCCACTGCACGCAGTG
TCGACACCAGTTCTGCAGTGGCTGCTACAACGCCTTTTACGCCAAGAATAAATGTCCAGACCCTAACTGC
AAGGTGAAAAAGTCCCTGCATGGCCACCACCCTCGAGACTGCCTCTTCTACCTACGGGACTGGACTGCTG
CCCGCCTCCAGAAACTGTTGCAGGACAATAATGTCATGTTTAATACAGAGCCTCCAGCTGGGACACGGGC
AGTCCCTGGAGGGGGCTGCAGAGTGATGGAGCAGAAGGAGGTCCATAGTGGGTTCAGGGATGAAGCTTGC
GGCAAGGAAACTCCACCTGGCTATGCCGGCCTATGTCAGGCACACTACAAAGAGTATCTCGTGAGCCTCA
TCAATGCCCATTCACTGGACCCAGCTACCTTGTATGAAGTGGAGGAGCTGGAGACAGCCACTATTCGCTA
CCTACATTTAGCTCCTCAGCCCGCGGATGGAGAGGATCTGCCTGCTTACCAGGCCCGGCTATTACAGAAG
CTGAGAGAAGAGGTACCCTTGGGACAGAGTATTGCCCGCAGAAGAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211571 protein sequence
Red=Cloning site Green=Tags(s)

MPGDEERGFLAAREELASALRWDSAQVFPLEQLMPLLATSLPPAARYLQLDAGRLVRCNAHGEPRNYLNT
LSTALNILEKYGRNLLSPQRPRYWRSVKFNNPVFRSTVDAVQGGRDVLRLYGYTEERPDGLSFPEGQEEP
DEYQVAVVTLEVLLLRTELSLLLQNTHPRQNALDQLLRESVEDGMLQLSEFHPLLREIVPGPRPSAQGST
PGPCFLCGSAPGTLHCPACNQVSCPACDILFHGHPSRAHHLRQALPGSHQTASLSSSLPASSQPRPPSSS
LALGDSSLSSPDPANACLPWHCLTCATLNEPWAVFCAVCSQPKGCKVPGIEGSHGTGGLEPEPARDQWAC
QSCTFENEAAAVLCAICERPRLAQPPSLVVDSHDAGVCQQSLKQEDPLLTAAQPQVWYCDHCTFCNSGPV
WVCAMCNRTRDPIPTQPALQSYPSSLEKGRPKPGSSQHLGSSLPASCGDPEKQRQDKMRKEGLQLVSMIQ
EGETAGASPEEVFSALQYSGTEVPLQWLRSELSYVLEMVAELAGQQDPELGAFSCQEARKAWLDRHGNLD
EAVEECVRARRRKVHELQSLGFGPKEGSLQALFQHGGDVARALTELQRQRLEPFHQRLWDRDPEPTPCWD
GLDRQSLVRRLLAVYTLPSWGRAELALALLQETPRNYELLDVVEAVRHSQDRAFLRRLLAQECAVCGWAL
PRNRMQALISCECTICPECFRQHFTIALKEKHITDMVCPACGRPDLTDDAQLLSYFSTLDIQLRESLDPD
AYALFHKKLTEAVLMRDPKFLWCAQCSFGFIYEREQLEATCPQCHQTFCVRCKRQWEEQHRGRSCEDFQN
WKRTNDPEYQAQGLAMYLQENGIDCPKCKFSYALARGGCMHFHCTQCRHQFCSGCYNAFYAKNKCPDPNC
KVKKSLHGHHPRDCLFYLRDWTAARLQKLLQDNNVMFNTEPPAGTRAVPGGGCRVMEQKEVHSGFRDEAC
GKETPPGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATIRYLHLAPQPADGEDLPAYQARLLQK
LREEVPLGQSIARRRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_194346
ORF Size 3198 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_194346.3
RefSeq Size 3443 bp
RefSeq ORF 3201 bp
Locus ID 268749
UniProt ID Q924T7
Cytogenetics 14 C3
MW 119.3 kDa
Summary E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation (PubMed:28701375). LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways (By similarity). Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation (PubMed:28701375). Recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex (By similarity). Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis (By similarity). Binds polyubiquitin of different linkage types (By similarity).UniProtKB/Swiss-Prot Function
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"NM_194346" in other vectors (4)
SKU Description Size Price
MC205166 Rnf31 (untagged) - Mouse ring finger protein 31 (Rnf31), (10ug) 10 ug
$929.00
MG211571 Rnf31 (tGFP-tagged) - Mouse ring finger protein 31 (Rnf31) 10 ug
$789.00 $1,129.00
MR211571L3 Lenti ORF clone of Rnf31 (Myc-DDK-tagged) - Mouse ring finger protein 31 (Rnf31) 10 ug
$1,229.00
MR211571L4 Lenti ORF clone of Rnf31 (mGFP-tagged) - Mouse ring finger protein 31 (Rnf31) 10 ug
$1,229.00

Plasmid Map for MR211571

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211571
Circular map for MR211571

file-alt Citations

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