Skiv2l2 (NM_028151) Mouse Tagged ORF Clone

SKU MR211508

Skiv2l2 (Myc-DDK-tagged) - Mouse superkiller viralicidic activity 2-like 2 (S. cerevisiae) (Skiv2l2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Skiv2l2
• Synonyms: 2610528A15Rik; mKIAA0052; Mtrex
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211508 representing NM_028151; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGATGCTTTCGGGGATGAGCTTTTCAGCGTTTTCGAGGATGATTCGACCTCTGCAGCAGGAGCCA; AGAAAGACAAAGAGAAAGAGAAATGGAAGGGGCCACCAGGGTCTGCAGACAAGGCCGGGAAACGACTGGA; TACCAAATTACAATCAGAGTCAGCCAGTGGGGGGAAAAACAAAAGAGATCTAGATGTTGAAGGCACTGAT; GAACCTATTTTTGGCAAGAAGCCGAGAATAGAAGACTCAATTAATGAAGATTTAAGCCTGGCGGACCTGA; TGCCCAGAGTCAAGGTACAGTCAGTGGAAACAGTTGAAGGCTGCACACATGAGGTCGCTCTTCCTGCAGA; TGAGGATTATATACCACTTAAACCTCGAGTTGGGAAGGCAGCAAAGGAATACCCATTCATTCTTGATGCT; TTCCAAAGAGAAGCCATTCAGTGTGTTGATAATAACCAGTCTGTGCTAGTATCTGCTCATACGTCAGCAG; GGAAGACCGTGTGTGCCGAGTATGCTATTGCATTGGCTTTAAGAGAAAAACAGCGTGTAATATTTACCAG; CCCTATTAAGGCTCTAAGTAATCAGAAATACCGTGAAATGTATGAAGAATTTCAAGATGTGGGGCTGATG; ACGGGAGATGTTACTATTAATCCCACAGCATCTTGTCTCGTTATGACTACAGAGATTTTGAGAAGTATGC; TGTATAGAGGCTCTGAAGTTATGCGAGAAGTTGCTTGGGTCATATTTGATGAAATTCATTACATGAGAGA; TTCAGAGCGTGGTGTAGTATGGGAAGAAACTATTATTTTGCTTCCAGATAATGTTCACTATGTCTTTCTT; TCGGCTACTATTCCAAATGCTCGACAGTTTGCTGAATGGATTTGTCACCTACATAAACAGCCCTGTCATG; TTATTTACACTGATTATCGACCCACTCCACTGCAACACTACATATTTCCAGCAGGGGGAGATGGCCTTCA; CCTGGTGGTTGATGAAAATGGTGACTTCAGAGAAGATAATTTTAACACTGCAATGCAAGTGCTGCGGGAT; GCAGGTGATTTGGCCAAAGGAGACCAGAAGGGACGGAAAGGAGGAACAAAAGGTCCATCAAATGTGTTCA; AGATTGTGAAGATGATTATGGAGAGAAATTTCCAGCCTGTGATCATCTTCAGTTTCAGTAAGAAGGACTG; TGAAGCATATGCTCTTCAGATGACGAAGCTAGACTTCAACACAGATGAAGAAAAGAAGATGGTTGAAGAA; GTTTTCAATAATGCAATTGACTGCTTGTCTGATGAAGATAAAAAGCTCCCTCAGGTTGAACATGTGCTTC; CACTTTTGAAGCGGGGGATTGGTATTCACCATGGTGGCTTACTTCCTATTTTGAAAGAAACTATAGAAAT; TCTCTTTTCTGAAGGATTGATAAAGGCTTTATTTGCCACAGAGACTTTTGCTATGGGAATTAACATGCCA; GCTAGAACTGTTTTATTTACAAATGCCCGTAAATATGACGGAAAGGACTTCAGATGGATTTCCTCTGGCG; AGTACATCCAGATGTCTGGTCGCGCAGGAAGGAGAGGCATGGACGACAGAGGGATTGTGATTCTTATGGT; AGACGAGAAGATGAGCCCAACTATTGGAAAGCAGCTGCTTAAGGGGTCAGCTGATCCTCTGAACAGTGCT; TTCCATCTGACCTATAACATGGTTTTGAACTTACTAAGAGTAGAAGAAATTAACCCCGAGTACATGCTTG; AAAAATCCTTCTACCAGTTTCAGCATTATAGAGCAATTCCAGGGGTCGTAGAAAAGGTAAAGAACTCAGA; AGAGCAGTATAATAAAATAGTCATTCCCAATGAAGAAAATGTGGTCATCTATTACAAGATTAGACAGCAA; CTTGCCAAGCTGGGAAAAGAGATTGAAGAATATATTCATAAACCAAAGTACTGCTTACCCTTTCTACAGC; CAGGCCGTTTGGTAAAGGTGAAAAATGAAGGCGATGATTTCGGCTGGGGAGTAGTAGTGAACTTCTCAAA; AAAGTCAAATGTCAAGCCCAATTCTGGCGAACTGGATCCCTTGTATGTGGTGGAAGTCCTCCTGCGCTGT; AGCAAGGAAAGCCTGAAGAACTCAGCCACCGAGGCAGCAAAGCCAGCGAAGCCAGACGAGAAAGGGGAGA; TGCAGGTTGTTCCAGTTTTGGTGCATCTCCTGTCTGCCATCAGCACTGTCAGGCTCTATATTCCAAAAGA; CCTTCGACCTGTGGACAACAGACAGAGTGTCTTAAAATCCATACAGGAAGTTCAAAGGCGCTTTCCCGAC; GGCGTTCCTCTGTTAGATCCGATCGATGATATGGGCATCCAAGATCAAGGACTGAAGAAAGTCATCCAGA; AAGTAGAGGCCTTTGAGCATCGCATGTACTCACACCCACTCCACAATGATCCTAACTTGGAAACTGTTTA; TACACTGTGTGAAAGAAAGGCCCAGATTGCACTAGATATCAAGTCTGCAAAGAGAGAACTGAAGAAGGCA; AGAACTGTTCTGCAAATGGACGAGCTCAAATGCCGCAAGCGCGTGCTGAGACGGCTGGGCTTTGCGACAT; CGTCGGATGTCATAGAGATGAAAGGACGGGTGGCTTGTGAAATAAGCAGTGCGGACGAGCTCCTTCTAAC; GGAGATGATGTTCAATGGGCTTTTCAATGACCTTTCTTCAGAGCAGGCAACCGCACTGTTAAGCTGTTTT; GTGTTTCAAGAGAATTCTAGTGAGATGCCCAAACTAACAGAGCAGTTAGCTGGACCACTCCGACAAATGC; AGGAATGTGCTAAAAGGATTGCAAAAGTTTCAGCAGAGGCAAAGCTGGAGATTGATGAAGAAACTTACCT; AAGCTCATTCAAGCCTCACCTGATGGATGTGGTGTATACCTGGGCAACTGGGGCCACATTTGCCCATATC; TGCAAAATGACAGATGTTTTCGAAGGCAGTATAATTCGTTGTATGAGGCGTCTGGAAGAACTGCTTCGAC; AGATGTGTCAAGCAGCAAAAGCCATTGGAAACACAGAGCTGGAGAACAAATTTGCTGAAGGAATTACCAA; AATCAAGAGGGATATTGTATTTGCTGCCAGCCTCTACTTA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211508 representing NM_028151; Red=Cloning site Green=Tags(s); ; MADAFGDELFSVFEDDSTSAAGAKKDKEKEKWKGPPGSADKAGKRLDTKLQSESASGGKNKRDLDVEGTD; EPIFGKKPRIEDSINEDLSLADLMPRVKVQSVETVEGCTHEVALPADEDYIPLKPRVGKAAKEYPFILDA; FQREAIQCVDNNQSVLVSAHTSAGKTVCAEYAIALALREKQRVIFTSPIKALSNQKYREMYEEFQDVGLM; TGDVTINPTASCLVMTTEILRSMLYRGSEVMREVAWVIFDEIHYMRDSERGVVWEETIILLPDNVHYVFL; SATIPNARQFAEWICHLHKQPCHVIYTDYRPTPLQHYIFPAGGDGLHLVVDENGDFREDNFNTAMQVLRD; AGDLAKGDQKGRKGGTKGPSNVFKIVKMIMERNFQPVIIFSFSKKDCEAYALQMTKLDFNTDEEKKMVEE; VFNNAIDCLSDEDKKLPQVEHVLPLLKRGIGIHHGGLLPILKETIEILFSEGLIKALFATETFAMGINMP; ARTVLFTNARKYDGKDFRWISSGEYIQMSGRAGRRGMDDRGIVILMVDEKMSPTIGKQLLKGSADPLNSA; FHLTYNMVLNLLRVEEINPEYMLEKSFYQFQHYRAIPGVVEKVKNSEEQYNKIVIPNEENVVIYYKIRQQ; LAKLGKEIEEYIHKPKYCLPFLQPGRLVKVKNEGDDFGWGVVVNFSKKSNVKPNSGELDPLYVVEVLLRC; SKESLKNSATEAAKPAKPDEKGEMQVVPVLVHLLSAISTVRLYIPKDLRPVDNRQSVLKSIQEVQRRFPD; GVPLLDPIDDMGIQDQGLKKVIQKVEAFEHRMYSHPLHNDPNLETVYTLCERKAQIALDIKSAKRELKKA; RTVLQMDELKCRKRVLRRLGFATSSDVIEMKGRVACEISSADELLLTEMMFNGLFNDLSSEQATALLSCF; VFQENSSEMPKLTEQLAGPLRQMQECAKRIAKVSAEAKLEIDEETYLSSFKPHLMDVVYTWATGATFAHI; CKMTDVFEGSIIRCMRRLEELLRQMCQAAKAIGNTELENKFAEGITKIKRDIVFAASLYL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028151
• ORF Size: 3120 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_028151.2, NP_082427.1
• RefSeq Size: 3309 bp
• RefSeq ORF: 3123 bp
• Locus ID: 72198
• UniProt ID: Q9CZU3
• Cytogenetics: 13 D2.2
• MW: 117.6 kDa
• Summary: Component of exosome targeting complexes. Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. Associated with the RNA exosome complex and involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. May be involved in pre-mRNA splicing.[UniProtKB/Swiss-Prot Function]