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Usp15 (NM_027604) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211381
Usp15 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $899.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Usp15
Synonyms 4921514G19Rik; AI327321; E430033I05Rik; Gcap; Gcap18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211381 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGGCGGAGCGGCGGACCTGGACACCCAGCGTTCTGACATCGCGACGCTGCTCAAAACCTCGC
TCCGGAAAGGGGACACCTGGTATCTAGTAGATAGTCGGTGGTTCAAACAGTGGAAAAAATATGTTGGCTT
TGACAGCTGGGACAAATACCAGATGGGAGATCAAAATGTCTATCCTGGACCCATCGATAACTCTGGACTT
CTCAAAGATGGCGACGCGCAGTCACTTAAGGAGCACCTTATTGATGAGTTGGATTACATACTCTTGCCAA
CTGAGGGCTGGAATAAACTTGTCAGCTGGTACACACTGATGGAGGGCCAGGAGCCAATAGCAAGAAAGGT
GGTAGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTCTACCTCACAGAACTGAAGCTCTGTGAG
AACGGGAACATGAACAATGTTGTAACTCGGAGGTTTAGCAAAGCTGACACAATAGATACGATTGAGAAGG
AAATAAGAAAAATCTTCAATATTCCAGATGAGAAGGAGGCCAGACTGTGGAACAAGTATGTGAGCAACAC
ATTTGAACCACTGAATAAGCCAGACAGCACCATCCAGGATGCCGGCCTATACCAAGGACAGGTGTTAGTG
ATAGAGCAAAAAAATGAAGATGGAACGTGGCCCAGGGGTCCCTCTACTCCTAAGTCCCCAGGTGCATCCA
ATTTTTCAACTTTACCAAAGATCTCTCCTTCATCTCTATCAAATAATTATAACAACATCAACAACAGAAA
TGTGAAAAACTCAAATTATTGTCTCCCGTCATATACTGCTTATAAGAACTATGATTATTCAGAACCTGGA
AGAAACAATGAACAGCCAGGCCTGTGTGGCTTAAGTAACTTGGGAAATACGTGTTTCATGAACTCAGCCA
TTCAGTGTTTGAGCAACACACCTCCACTTACTGAATATTTCCTCAATGATAAGTACCAAGAAGAGCTGAA
TTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCATACGCTGAACTCATCAAGCAGATGTGG
TCTGGAAAGTTTAGCTACGTCACTCCAAGAGCATTCAAGACACAGGTAGGGCGCTTCGCACCTCAGTTCT
CTGGATATCAGCAGCAGGACTGCCAAGAACTACTGGCTTTCCTGTTGGATGGACTGCACGAGGACCTGAA
CAGAATTAGGAAAAAACCCTATATCCAGTTAAAAGATGCTGATGGCAGGCCAGACAAGGTGGTTGCTGAA
GAAGCCTGGGAGAACCATTTAAAGCGAAATGATTCCATCATAGTAGACATATTCCATGGCCTTTTCAAGT
CTACGTTAGTGTGTCCTGAGTGTGCTAAGATCTCGGTGACCTTTGACCCTTTCTGTTACTTGACTCTTCC
ATTGCCTATGAAAAAAGAACGTAGCTTGGAAGTTTATTTAGTTAGAATGGATCCACTTGCTAAACCTATG
CAATACAAAGTTATTGTGCCCAAAATTGGAAATATACTAGATCTTTGTACAGCGCTGTCTGCTTTGTCGG
GAGTACCTGCAGATAAGATGATAGTCACTGACATATACAATCATAGATTTCACAGGATATTTGCCGTGGA
TGAAAACCTGAGTAGTATTATGGAGCGGGATGATATTTACGTGTTTGAAATTAACATCAACAGGGCAGAA
GACACAGAGCATGTAGTCATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCAAGTTACACGCACCACA
CTGGCTCTTCACTGTTCGGCCAGCCTTTTCTTATGGCTATACCACGAAACAATACTGAAGATAAACTCTA
TAATCTTCTACTCTTGAGAATGTGCCGGTATGTCAAAATGTCTACTGAAACTGAGGAAACAGATGGACAC
CTACGTTGCTGTGAGGACCAGAATATTAATGGGAATGGGCCAAATGGCCTACATGAAGAGGGTTCACCAA
GTGAGATGGAAACCGACGAGCCAGGTGACGAGTCCAGCCAGGATCAAGAGCTTCCCTCAGAGAACGAGAA
CAGTCAGTCTGAAGATTCAGTCGGAGGAGATAACGATTCTGAAAATGGATTGTGCACTGAAGAAACATGC
AAAGGTCAACTTACGGGACACAAAAAGCGCTTGTTTACATTCCAGTTCAACAACTTAGGCAACAATGACA
TCAACTACATCAAAGACGACACCAGTCACATCCGATTCGATGACAGGCAGCTTCGGCTGGACGAAAGATC
TTTTCTCGCTTTGGACTGGGACCCTGACTTGAAGAAGAGATACTTTGATGAGAATGCTGCAGAGGATTTT
GAAAAACATGAAAGTGTGGAATACAAACCTCCAAAAAGGCCCTTTGTGAAGTTAAAAGACTGCATTGAGC
TCTTCACCACCAAGGAGGAGTTGGGTGCTGAGGACCCCTGGTATTGTCCAAATTGTAAAGAGCATCAGCA
AGCCACAAAGAAATTAGATCTGTGGTCCCTGCCTCCAGTTCTTGTGGTGCATCTCAAGAGATTCTCCTAT
AGTCGCTACATGAGAGACAAGCTAGATACATTAGTCGATTTTCCCATCAGTGACTTGGATATGTCAGAAT
TCCTAATTAACCCAAATGCAGGTCCTTGCCGCTATAATTTGATTGCTGTTTCCAACCACTATGGAGGGAT
GGGAGGAGGACATTATACTGCTTTTGCAAAAAACAAAGATGATGGGAAGTGGTACTATTTCGATGACAGT
AGCGTCTCCACTGCATCTGAAGACCAAATCGTGTCCAAGGCAGCGTACGTGCTCTTCTACCAGAGACAGG
ACACTTTCAGTGGGACTGGCTTCTTCCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACAGGTAT
CCCCCTGGAAAGTGACGAAGACAGCAATGATAATGACAATGACCTAGAAAATGAAAACTGTATGCACACT
AAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211381 protein sequence
Red=Cloning site Green=Tags(s)

MAEGGAADLDTQRSDIATLLKTSLRKGDTWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGL
LKDGDAQSLKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCKVEVYLTELKLCE
NGNMNNVVTRRFSKADTIDTIEKEIRKIFNIPDEKEARLWNKYVSNTFEPLNKPDSTIQDAGLYQGQVLV
IEQKNEDGTWPRGPSTPKSPGASNFSTLPKISPSSLSNNYNNINNRNVKNSNYCLPSYTAYKNYDYSEPG
RNNEQPGLCGLSNLGNTCFMNSAIQCLSNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMW
SGKFSYVTPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQLKDADGRPDKVVAE
EAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVTFDPFCYLTLPLPMKKERSLEVYLVRMDPLAKPM
QYKVIVPKIGNILDLCTALSALSGVPADKMIVTDIYNHRFHRIFAVDENLSSIMERDDIYVFEININRAE
DTEHVVIPVCLREKFRHSSYTHHTGSSLFGQPFLMAIPRNNTEDKLYNLLLLRMCRYVKMSTETEETDGH
LRCCEDQNINGNGPNGLHEEGSPSEMETDEPGDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEETC
KGQLTGHKKRLFTFQFNNLGNNDINYIKDDTSHIRFDDRQLRLDERSFLALDWDPDLKKRYFDENAAEDF
EKHESVEYKPPKRPFVKLKDCIELFTTKEELGAEDPWYCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSY
SRYMRDKLDTLVDFPISDLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYFDDS
SVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGIPLESDEDSNDNDNDLENENCMHT
N

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027604
ORF Size 2943 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027604.2, NM_027604.3, NM_027604.4, NP_081880.2
RefSeq Size 11564 bp
RefSeq ORF 2946 bp
Locus ID 14479
UniProt ID Q8R5H1
Cytogenetics 10 D2
MW 112.2 kDa
Summary The protein encoded by this gene is a member of the large ubiquitin specific protease (Usp) family of proteins. These proteins are known to cleave ubiquitin, and contain a conserved cysteine residue (Cys box) and two conserved histidine residues (His box) that are thought to form part of the active site of the protease. This protein has been shown to cleave both the ubiquitin-proline and the ubiquitin-methionine bonds in vitro. This protein is thought to regulate many cellular processes through its deubiquitination activity, including the transforming growth factor beta (TGF-beta) pathway. Cardiac-specific overexpression of the human ortholog of this gene in mice causes enlargement of the heart that is more pronounced in the atrium than in the ventricle. This gene has two pseudogenes on chromosome 14. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms.provided by RefSeq, Aug 2014
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"NM_027604" in other vectors (3)
SKU Description Size Price
MG211381 Usp15 (tGFP-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15) 10 ug
$1,099.00
MR211381L3 Lenti ORF clone of Usp15 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15) 10 ug
$1,199.00
MR211381L4 Lenti ORF clone of Usp15 (mGFP-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15) 10 ug
$1,199.00

Plasmid Map for MR211381

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211381
Circular map for MR211381

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