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Prrt3 (NM_172487) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211350
Prrt3 (Myc-DDK-tagged) - Mouse proline-rich transmembrane protein 3 (Prrt3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $889.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Prrt3
Synonyms 6330505P20; B230206N24Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211350 representing NM_172487
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCCCAGCCCACAGGCCTGCACATCTCCTCTTCTGCTGTTGCTGCTGCCATGCCTGGGGGCTGGCC
CTGCCTTGGGCAGAGGCCTTCCCAGGCCCCTGGAGAACTCAGAACCTCACATGATCCCATCAGAGTCACA
GACCTTTGACCTCTTCTGGGAGAAGCTGAGAAATGAGAGTTCCTGGCACAGCGGTGACCCTCAGGCCCGT
GCTGAGGGGCCCAAGAAGCCCGCAGACCCTTACCTAGGACCAGCCCTGCATGGGCCCAAGGCAGCCCCTG
GGGTCCAAGGTGAAAGACTACTAAGAGCTGATGACCTCCAGCTGGCCCGAGCATTCACCTCCCAAGGCTG
GACAGGACCTCCTGACTCACAGGAGCTTCTGGAGCCTGAGGCACCAGAACCCCACCCTGTGCGAGCACCT
CGTCTCACTCTTGTCACTACAACTCCCAGCTCCCTACTCTCGGCAGCCATACTTTCCACTGCCTCCCAGA
AGCCTGGAGGCACAGCAGGGCAGCAGCCAGCTAGGAACGAGGAGCTAATAATGGTCAAAGCCGAGACCCA
CATCACACAGGCTTCCCCCTGGGACTTCCAGGGCTCTTCCCACACTCCGGTACCCGAGACAGATGCTGTG
AGGACGCTGGTGTTGGGGAAGCAGGGGGGACATGAACAGGGCTTCCAGGAGGCTGTCCAGGGTCCCCTAC
TCACCCAGCAGGATCCAGTAGTCCCTGGGGTCGGCTCAACACCCCCAGTTAAAGTGGAATCCACCCCAGA
GCCTGGAGCCCAGCTCGACCTGGCATTGGTCAGAAGCCTTCCTCTTCCTGAGGGGCTGCCAGCTGAACCC
CCCAAAACTGGAGCTGGGGATACCTGGGAAGTCAGCTCTCTGGGACCCCAACCAGAACAGACCGATCTCC
TTGGAGGACAAGATTCCCCAGCACCCCAGCCTATACCACCTTCAGCCTCAGACACTTCTGATGGGCACCT
AAGACCAGTATCCTCGCTGAATGGAGCCGACCCCATCTCCCCACAACGTGTGAGAGGAGCGATGGAGGCC
CCAGGAACCCCCAAGTCCTTCATCCCTGACCTTCCAAATTCTGCTCAAGCTGCAAATGGAACAGAGAGCC
CTGTGAGGGCCCTGCAGCCAGATGAAGCCGAGGATTGGCCGGGTCGCCCACAAAGCCACCCTCCAGCACC
CCCTGTCCAGGCCCCCTCGACATCTCGGCGTGGTCTCGTTAGAGTCACCACACAGAGAGCTCTGGGCCAG
CCACTCCCTCCGGAGCCCTCAGCCAGCTCCATAGTCCCCATCCCAGCTTCTAGCCCCCCAGCCAATGCCA
CCGCACCTCCCCTGCGTTGGGGACCCCTTCGGCGGGTGCTGAGTTTCTCCTGGGAGCTTCATGTGTATGG
GGTGGGGGTGCTTTTCCTGCTTCCAGCATTATTGGCATTGGTCACAGTGGCAGCCGCCCTAGCGGGACCC
CGATTAGCACTAGTGGCTGCCGCGCTGGTATTGGTGGCTTCAGGGCTGCGATCTGCCTACATGCTCACGG
ATCCTTATGGCTCTCAGGCGCGACTGGGAGTACGTGCAGGTCTGGTGCTATACAACTTGCCCTTCCCCTT
GCTACTCACTGCACTGGCAGCACTCACCCTGCTGGGCTTGGGTGCTGGTCTGCCACAGCCTCTGCAGAAG
CCGCTGCTGCTGGGAATTGTGGCACCGGTGCATGGCACATGTTTGCTGGCTACAGACCTGTTCTCCACCA
GCCCTGTGCTCAACCTTCTAACACAAGGCTTATCTTGCGCCTGGGGCGCCTCAGTGGCTCTGGGCACACT
CTGCCTGTGCCGTCGACGCCTGCTGGAAGGCCCTCGGGGCTGGGATGCCAGTCCAGGGCCCCGGCTGTTG
GCTGTGGCGGGCTCACTGGGGCTGTTGGCCAGTGGTCTGCAGTTGGCGGCCTCTCTCTGGCTGTACCCAG
GGCCTGGCCGGGAGGGTCGCTTCTCCTGGGCTTGGTGGGGCGTGCACTTCTGGCTGCGCCTGCTGGAATT
GACCTGGGCACTGGCCCTGGCGCTAGCTGCCCTGGCTGCCACTCGTCCCAGGCCGCCCACGGAGCACGCT
TGCTGGGCTAAGCTGCTGCGTCTGGCGTGCCCCGCGCCTACAGGGAAGAGCGAAGTTCCCGAGAGACCCA
ATAACTGCTATGCGGGGCCCAGTGGCCTGGGCACAGGCGGCCTGGACATCAGCAAAAGTCTCATCCGCAA
CGCTGCTGGGGAAGCTGGGCTTCCTGTCACTCCTGGTTCTGGACCCTGGGGTTCGGCTGCATCGCTGGGT
CGCGGTCGTCCAGGTGGCCAGAGGATGTCCCGGGGCAGTGTGGGGCCCGCACCGTCGCTGAGCGAGCTGG
ATCTGCGGCCACCGTCACCTATCAATTTGAGCCGCAGCATCGACGCCGCGCTCTTCCGGGAGCACTTGGT
TCGAGAGAGTGTGTTCCAACGCTGTGGTCTCCGTGGCTTGGCCTCCTCGCCGACAGGGGGCGCCCTGCGG
CCTCGCCGCGGCAGCCAGCCCGATGCCGAGCTCGACGGCGCGGGCACATCATTGCTCCGAGGCCGCTGCC
GCTCGCTCACCGAGGTGTGCTTGCGCACGTCGCTCCCGCAGCACGTGATGGAGCCGCCCGTCGGCGCGGC
GGCCGCGGGGACTTCGGGCAGCTCCCTGGACAGTTTCTCCAAGGGCTCGCTGAAGATCAGCTGGAACCCG
TGGCGTCACGGGCTGTCATCCGTGGACAGCCTGCCCCTAGATGAACTGCCCAGCACCGTGCAGCTGCTGC
CACCACCTACCCCAGTCCCTGCTCCTGCCCGGGCGGGGGAGCCTCAGGGCGAGGGCCAGTCGCGTTGCAA
GTCCAGCGAGTCTCACAGCGCCTCTAGTGACACTATAGAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211350 representing NM_172487
Red=Cloning site Green=Tags(s)

MAPSPQACTSPLLLLLLPCLGAGPALGRGLPRPLENSEPHMIPSESQTFDLFWEKLRNESSWHSGDPQAR
AEGPKKPADPYLGPALHGPKAAPGVQGERLLRADDLQLARAFTSQGWTGPPDSQELLEPEAPEPHPVRAP
RLTLVTTTPSSLLSAAILSTASQKPGGTAGQQPARNEELIMVKAETHITQASPWDFQGSSHTPVPETDAV
RTLVLGKQGGHEQGFQEAVQGPLLTQQDPVVPGVGSTPPVKVESTPEPGAQLDLALVRSLPLPEGLPAEP
PKTGAGDTWEVSSLGPQPEQTDLLGGQDSPAPQPIPPSASDTSDGHLRPVSSLNGADPISPQRVRGAMEA
PGTPKSFIPDLPNSAQAANGTESPVRALQPDEAEDWPGRPQSHPPAPPVQAPSTSRRGLVRVTTQRALGQ
PLPPEPSASSIVPIPASSPPANATAPPLRWGPLRRVLSFSWELHVYGVGVLFLLPALLALVTVAAALAGP
RLALVAAALVLVASGLRSAYMLTDPYGSQARLGVRAGLVLYNLPFPLLLTALAALTLLGLGAGLPQPLQK
PLLLGIVAPVHGTCLLATDLFSTSPVLNLLTQGLSCAWGASVALGTLCLCRRRLLEGPRGWDASPGPRLL
AVAGSLGLLASGLQLAASLWLYPGPGREGRFSWAWWGVHFWLRLLELTWALALALAALAATRPRPPTEHA
CWAKLLRLACPAPTGKSEVPERPNNCYAGPSGLGTGGLDISKSLIRNAAGEAGLPVTPGSGPWGSAASLG
RGRPGGQRMSRGSVGPAPSLSELDLRPPSPINLSRSIDAALFREHLVRESVFQRCGLRGLASSPTGGALR
PRRGSQPDAELDGAGTSLLRGRCRSLTEVCLRTSLPQHVMEPPVGAAAAGTSGSSLDSFSKGSLKISWNP
WRHGLSSVDSLPLDELPSTVQLLPPPTPVPAPARAGEPQGEGQSRCKSSESHSASSDTIEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172487
ORF Size 2913 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172487.4, NP_766075.2
RefSeq Size 3304 bp
RefSeq ORF 2916 bp
Locus ID 210673
UniProt ID Q6PE13
Cytogenetics 6 E3
MW 101.7 kDa
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Documents
Resources
"NM_172487" in other vectors (4)
SKU Description Size Price
MC222922 Prrt3 (untagged) - Mouse proline-rich transmembrane protein 3 (Prrt3), (10ug) 10 ug
$979.00
MG211350 Prrt3 (tGFP-tagged) - Mouse proline-rich transmembrane protein 3 (Prrt3) 10 ug
$1,089.00
MR211350L3 Lenti ORF clone of Prrt3 (Myc-DDK-tagged) - Mouse proline-rich transmembrane protein 3 (Prrt3) 10 ug
$1,189.00
MR211350L4 Lenti ORF clone of Prrt3 (mGFP-tagged) - Mouse proline-rich transmembrane protein 3 (Prrt3) 10 ug
$1,189.00

Plasmid Map for MR211350

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211350
Circular map for MR211350

file-alt Citations

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