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Chtf18 (NM_145409) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211343
Chtf18 (Myc-DDK-tagged) - Mouse CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (Chtf18)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $888.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chtf18
Synonyms 6030457M03Rik; Chl12; CTF18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211343 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGACTACGAGGAAGATCTGTATGGTGTGGAGGATGATTTCCAAAACCAGTTCGCAGCTGAGCTCG
AAGTGCTGGCTGAGCTGGAAGGAACACGGGACCAGGCTCCTCCTGGGACCCTCCAAACTCCAGCGAGCCG
CCCTCCGCTAACCTTCGAAGAGGCCATCGCTGGTGGGGACACTGTCCCGCGTCCCTGCCCTGCAGGGTCG
CCAGGAAATGTCAACCGCAATACTAGAAAGAATGTTCGTAGGGACCAGCCCGCGCCCTCCAGCCCCATGG
TCAAACGACCCAGGTTGGACGTGGTGAAGAAGCTGAACTTTGAGCCAGATATGGAGGAGCTCTTATATCC
AGATTCCCCTCCTGGGGACATTACCCCTCCACCGAGTCCAGAGGTCTTCCCTGAGATGTTGGATGCTGGG
TATTCAGATGCTAATGCTGACAAAGACCTCATGCAGACATTGCCAAGTCCCCGAAATCGCAATCCTGTCC
TGAGACGGCCTCCTATCTTAGAAGATTATATCAATGTGACATCCACAAGTGGAGAACGGGCCTTTTTGGT
GCTTCGGGCTGACCTCATAGGCCCTGGGGTGCAGAACCCTCTTCTAGATGTCCACTGGCGAGGCTGTGGC
CAACTGGACCTGCTGGGTGTCCCCTTCGCCTCCCTGAAGGAGCAAGTTGATAGCAAGAGACGGCAACAAC
TGCTGGAGGATGCCCAACAGCTTTCAGACACTCTACACAGCCTCAGGTCTGAAGGAGAGGAGGCTGTGCT
TGAAGGGCCCCCTGCGGAGGAGCCAGCCCCTGGCCAGAACACCGCTCAGCACTGCCTCTGGGTGGATGAG
TTTGCACCTCAGCATTACACGGAACTGCTCAGTGACGATTTTACCAACCGCTGCCTACTCAAGTGGCTGA
AGCTATGGGACCTTGTGGTGTTTGGCCGTGAGAGACCTGCCCGGAAGCCCAGACCTGGTGTAGAGACCAC
CCGGGTTGGGAAGGAGGCTACAGCCCCTGGCAAGTGGAAAAGCCATGAGCAGGCACTGGAGGAGATGCTA
GAAGCTGAACTGGACCCTAGCCAGAGGCCTCGGCAGAAGGTGGCACTGTTGTGTGGCCCTCCAGGACTAG
GCAAGACCACGCTGGCACATGTGGTCGCAAGGCATGCAGGGTATTGCGTGGTAGAGATGAATGCAAGTGA
TGACCGTAGCCCGGAGGCCTTCCGTACACGCATTGAAGCAGCTACGCAAATGGAGTCGGTTCTGGGTGTG
GGTGGGAGGCCCAACTGCCTGGTTATTGATGAGATCGACGGGGCCCCCACGGCGGCCATCAACGTTCTTT
TGGGTATCCTGAATCGCAAGGGTCCGCAGGAGGCCGACCAAGGAGGCACAGCTGTGGCCGCAGGGGGGCG
GCGGCGCAGGGCCGAGGGGGGTCTCCTAACCAGGCCTATCATCTGCATCTGCAATGATCAGTTCACACCT
TCTCTGAGGCAGCTGAAGCAGCAGGCTCTTCTGCTCCACGTCCCCCCAACTCTGCCCTCCAGGCTGGTGC
AGCGGCTCCAGGAGATCTCCCTGCAGCATGGCATGCGCTCTGACCCGGGTGCACTGGTTGCCCTCTGTGA
GAAGACCGACAATGACATCCGTGCCTGCATCAACACCCTGCAGTTTCTGTATGGGAGGGGCCGGCGGGAG
CTGAGTGTGAAGGCTGTGCAGACCACTCATGTCGGCCTGAAGGACCAGCGCAAGGGGCTATTCTCTGTGT
GGCAAGAGGTCTTCCAGTTGCCCAGGACTCAAAGGAGAATCGTGGGCCAGGACCTGATGCTGCCAGCCCA
CGCTCTCCTGCTCAGCAATGGTGACAAGGGCTCTCTGACCCTGGCCTCCCAGCGCTTTTACCACATCCTG
CGTGTGACCACCTCTGCGGGCGAGCATGAGAAGGTTGTCCAGGGCCTGTTTGACAACTTTCTACGCCTTC
GGCTTCGGGACTCCAGCTTAAGCACCGTGTGCTGTGCCCTCGACTGGCTGGCCTTTGATGACCTGCTGGA
GCAGGCTGCCCACCGTGGCCAGAGCTTCCAGTTGCTGTGCTACTTGCCCTTCGTGCCTGCGGCCTTCCAC
GTGCTCTTTGCCTCCAGCCATGTGCCACGAATCACTTTCCCCAGCAGCCAGCAGGAGGCCCAGACTCGGA
TGAGCCAGACAAGGAACCACATACAGACACTGGTGTCAGGTATGGCACCGACCACGCGTAGCCGGGCCAC
ACCACAGGCCCTTGTTCTAGATACTCTCTGCCTGCTCCTGGATGTCCTCGCACCCAAGCTGCGCCCCGTG
AGCACACAGCTGTACAGTGCCCATGAGAAGCAGCAGTTGTCCTGCCTTGTGGGTACCATGCTTGCCTACA
GTCTCACCTACCACCAGGAGCGCACACCTGATGGGCAGTACCTCTACAAGCTGGAGCCGAATGTGGAAGA
GGTCTGCCGCTTCCCTGAACTACCTGCCCGAAAGCCCCTCACCTACCAGGCTAAGCAGCTTATTGCTCGG
GAGATTGAAATGGAGAAGATGCGCAGGGCAGAGGCTTTGGCCTGGGCTCGAAGTGGCCCCCAGGTGGACC
AGGGCTCCTCAGGGCCTGCAAGTCTGTGGACAGACAGTGGGGAAAAGGAGACAAGGCAGCCTGCCCCACG
TAACCATGAGCAGCGCCTAGAACACATCATGAAGAGAGCAACCGTGCAGGAGCAGCCTGAGAGGGACTTC
TTTGGACGTGTGGTCATCAGGAAAGTGGCAGTCCCGAGCAGAGAGGTTGAGGCCCCCCAGAAGGACGCGG
ACGAGTGGCGCATGGGTGTGGCAGTGGGCAGGAGTGAGGTGTGGTTCCGGTTCAACGAGGGCGTCTCAAA
TGCTGTTCGGCGCAGCCTGTACATCAGGGACCTGCTG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211343 protein sequence
Red=Cloning site Green=Tags(s)

MEDYEEDLYGVEDDFQNQFAAELEVLAELEGTRDQAPPGTLQTPASRPPLTFEEAIAGGDTVPRPCPAGS
PGNVNRNTRKNVRRDQPAPSSPMVKRPRLDVVKKLNFEPDMEELLYPDSPPGDITPPPSPEVFPEMLDAG
YSDANADKDLMQTLPSPRNRNPVLRRPPILEDYINVTSTSGERAFLVLRADLIGPGVQNPLLDVHWRGCG
QLDLLGVPFASLKEQVDSKRRQQLLEDAQQLSDTLHSLRSEGEEAVLEGPPAEEPAPGQNTAQHCLWVDE
FAPQHYTELLSDDFTNRCLLKWLKLWDLVVFGRERPARKPRPGVETTRVGKEATAPGKWKSHEQALEEML
EAELDPSQRPRQKVALLCGPPGLGKTTLAHVVARHAGYCVVEMNASDDRSPEAFRTRIEAATQMESVLGV
GGRPNCLVIDEIDGAPTAAINVLLGILNRKGPQEADQGGTAVAAGGRRRRAEGGLLTRPIICICNDQFTP
SLRQLKQQALLLHVPPTLPSRLVQRLQEISLQHGMRSDPGALVALCEKTDNDIRACINTLQFLYGRGRRE
LSVKAVQTTHVGLKDQRKGLFSVWQEVFQLPRTQRRIVGQDLMLPAHALLLSNGDKGSLTLASQRFYHIL
RVTTSAGEHEKVVQGLFDNFLRLRLRDSSLSTVCCALDWLAFDDLLEQAAHRGQSFQLLCYLPFVPAAFH
VLFASSHVPRITFPSSQQEAQTRMSQTRNHIQTLVSGMAPTTRSRATPQALVLDTLCLLLDVLAPKLRPV
STQLYSAHEKQQLSCLVGTMLAYSLTYHQERTPDGQYLYKLEPNVEEVCRFPELPARKPLTYQAKQLIAR
EIEMEKMRRAEALAWARSGPQVDQGSSGPASLWTDSGEKETRQPAPRNHEQRLEHIMKRATVQEQPERDF
FGRVVIRKVAVPSREVEAPQKDADEWRMGVAVGRSEVWFRFNEGVSNAVRRSLYIRDLL

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_145409
ORF Size 2907 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145409.1, NP_663384.1
RefSeq Size 3163 bp
RefSeq ORF 2910 bp
Locus ID 214901
UniProt ID Q8BIW9
Cytogenetics 17 A3.3
MW 108.2 kDa
Summary Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145409" in other vectors (4)
SKU Description Size Price
MC201779 Chtf18 (untagged) - Mouse CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (Chtf18), (10ug) 10 ug
$888.00
MG211343 Chtf18 (tGFP-tagged) - Mouse CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (Chtf18) 10 ug
$1,088.00
MR211343L3 Lenti ORF clone of Chtf18 (Myc-DDK-tagged) - Mouse CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (Chtf18) 10 ug
$1,188.00
MR211343L4 Lenti ORF clone of Chtf18 (mGFP-tagged) - Mouse CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) (Chtf18) 10 ug
$1,188.00

Plasmid Map for MR211343

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211343
Circular map for MR211343

file-alt Citations

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