Slc8a3 (NM_001167920) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR211189
Slc8a3 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 1
$589.00
$843.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Slc8a3 |
| Synonyms | AW742262; Ncx3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001167920 |
| ORF Size | 2763 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001167920.1, NP_001161392.1 |
| RefSeq Size | 4943 bp |
| RefSeq ORF | 2766 bp |
| Locus ID | 110893 |
| UniProt ID | S4R2P9 |
| Cytogenetics | 12 37.44 cM |
| MW | 102.4 kDa |
| Summary | Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain (PubMed:14722618, PubMed:21593315). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:14722618, PubMed:21593315). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:14722618). In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (PubMed:21593315). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed:21959935). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (PubMed:24616101). Isoform 1 displays higher calcium exchanger activity than isoform 2, probably because isoform 1 has a lower threshold for activation by cytoplasmic Ca(2+) (PubMed:24616101).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001167920" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC220884 | Slc8a3 (untagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 1, (10ug) | 10 ug |
$928.00
|
|
| MR211189L3 | Lenti ORF clone of Slc8a3 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 1 | 10 ug |
$1,143.00
|
|
| MR211189L4 | Lenti ORF clone of Slc8a3 (mGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 1 | 10 ug |
$1,143.00
|
|
Citations
Sodium-calcium exchanger-3 regulates pain "wind-up": From human psychophysics to spinal mechanisms
Trendafilova, Adhikari, Schmid et al
Neuron (2022) 110 (16), 2571-2587.e13 DOI: 10.1016/j.neuron.2022.05.017
Trendafilova, Adhikari, Schmid et al
Neuron (2022) 110 (16), 2571-2587.e13 DOI: 10.1016/j.neuron.2022.05.017
Cited by 17
AI Summary: OriGene’s Slc8a3-B (Myc-DDK-tagged) plasmid (Cat# MR211189) was used in this study to investigate the role of the sodium-calcium exchanger NCX3 in pain "wind-up" and central sensitization mechanisms.
Snippet: Recombinant DNA ------------------------- pAAV CAG-GFP From Edward Boyden (Addgene plasmid # 37825;http://n2t.net/addgene:37825) RRID: Addgene_37825 Slc8a3-B (Myc-DDK-tagged) Origene Cat# MR211189 ------------------------- Software and algorithms ------------------------- Plink software for genetic data analysis and GWAS Chang et al., 2015 https://www.cog-genomic;Biotechne Cat# 317041-C2 Recombinant DNA pAAV CAG-GFP From Edward Boyden (Addgene plasmid # 37825;http://n2t.net/addgene:37825) RRID: Addgene_37825 Slc8a3-B (Myc-DDK-tagged) Origene Cat# MR211189 Software and algorithms Plink software for genetic data analysis and GWAS Chang et al., 2015 https://www.cog-genomics.org/plink/1.9/ R statistical software
Showing 1-1 of 1 papers.
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