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Ankrd24 (NM_027480) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211137
Ankrd24 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 24 (Ankrd24)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $902.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd24
Synonyms 4631433D01Rik; 5730519E19Rik; D10Bur2e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211137 representing NM_027480
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGACCCTCCGGGCACGATTCAAGAAAACAGAGGGCCAAGACTGGGGCAAAAGTGACCAGCGACTGC
TCCAGGCAGTGGAGAACAACGATGTGGCCAGAGTGGCCTCGTTGATCGCCCACAAGGGGCTGGTGCCTAC
AAAACTGGACCCCGAGGGCAAGTCTGCGTTCCACTTAGCAGCCATGAGGGGTGCGGCGGGGTGTCTGGAA
GTGATGCTGGCTCAAGGTGCCGATGTCATGAGCACGGATGGAGCAGGTTACAATGCCCTTCACCTGGCAG
CCAAGTACGGGCACCCAGAGTGTCTGAAGCAACTCTTGGAGGCTTCCTGCGTGGTGGACATCGAGGACAG
CAGTGGGTGGACAGCCCTTCACCATGCAGCGGCTGGTGGTTGTCTGTCCTGCTCAAAGCTGCTCTGTTCC
TTTAAGGCACACATGAACCCCCGGGACCGGTCGGGTGCCACACCCCTCATCATAGCGGCTCAGATGTGTC
ACACAGATCTGTGCCGCCTCCTCCTACAGCAGGGGGCTGCCACAAATGACCAGGACCTGCAAGGCAGGAC
AGCCTTGATGCTGGCATGTGAGGGGGGCAGCCCTGAGACTGTTGAAGTACTCCTGCAGGGTGGAGCCCAG
TTGAGCATCACTGATGCACTGGGCCAGGATGCCACTCACTACGGAGCCCTGACTGGAGACAAACTCATCT
TGCAGCTTCTACACGAGTCTGCACGGCGCTCTTCACCTCCCAGTGCCTCTCTAGAGGAGGACTCCGGAGA
GGCCTCATCCCAGAACTCAGTGTCAAGCCATGAGAAACAAGGAGCCCCCAAGAAGAGGAAGGCACCCCAG
CCTCCAGCCAGCACACCAGTTCCTGACGATCGGGATGCTTATGAGGAGATTGTACGCCTGAGACAAGAGA
GAGGCCGGCTCCTGCAAAAGATCAGGGGCCTGGAACAGCACAAGGAACGGAGGAGGAAGGAGCCCCTGGA
GGCGGAGGCCAGCTCAGTGCACAGCCTGGAGAGACAGGTGCAGGAGCTGCAACAGATGCTGGCGGAGAAG
CAGGAGGAGAAGGAGAGCCTGGGCCGGGAGGTGGAGAGCCTGCAGAGCCGTCTGTCCCTGCTGGAGAATG
AGAGAGAAAACACAAGCTATGATGTAGCTACCCTGCAGGATGAGGAGGGTGAGATGCCTGACTTCCCAGG
AGCTGATGCACTGATGCCAAAGAACCAGAGTCCATCAGCAGAGGAAATAGTTGCTTCCCTACAGGAGCAG
GTGGCTCAGCTCACCAGGCAGAACCAGGAGCTGCTGGAAAAGGTCCAGATCCTTGAGGAGTTTGAAAAGG
ATGAGGCGCAGATGGCAGAAGAAAGTCAGGCCGAGGTCGTCCCCCTGGTCTTGTATGAATCCTTACGGGC
AGAGCTTGAGCAGCTTCGGAGGCAATACACAGAGGCCATGCATTCGCAGCAGCAGCAGCAGGAGGGGGAG
CCACCCAGGGCTCAAGAAGGAGAAGAGACAGCATACCAGGAAATCAAGGATAAGGGAATCACTATCCAGA
ATGGACCCAGCGTCCCAGACCTCAACGGCACCACATATGCAGAAACCAAAGCAAATGGAATGGAACTCCA
AGCAGGAGGCTCCAAGGGTGTCTGGAATACTGAAGCAGGGGTTTCAGAAGCAGCACCCATAGAACCCGAG
GCTGCAGGTTCAGAGGCCACGGGGAAAGACAGACTGGCAGCCAAGGAAATGGACACTAGTGCTACTATGG
CTGAGGCCCTAAATGTGAAAGCTCTAGGTGACAATGCCGAAAGTGAGCCAGTGGCTGCCGAAGATACAGG
AGGAAAAGAGAATCCAGGAATGAAAGCTGATGAGGTGGATGTGTTGGCACAGGCAGGGCTCACAGGTACA
GTGATTAGAAACATGGAGGCCATCGGAGTGCGGGATACAGGAATTCAGGCCACAGGATTAGAGGCTAAGG
CAGTGAAGACCACTGGAGTGCAGGCCACAGTGGCGGAGGTCATAGGAGTGAAGGTCACAGGAGTGCAGAC
CACAGCGATAGAAGCTATAGGAGTCAAGGACACCACCCAGGTGGCCACAGGAGCGCAGGCCGATTGCTGG
CAGGCCACGGAGGCAGACTGCACCGGAGCGCAGGACACAGCTATGGAGCCCACGGGGGCACAGGCCACTG
TGACAGAGACTACGGAAGCCGAGACCAGCGGCACGGAGGACCCTTGCGCTGCGATCCTGCACCCGGGGGC
GGCGGCGGCGGCGCTGCAGGCCGAGCTGGAGACCCGGATCCGCGGTCTGGAGGAGGCGCTACGCAGAAGG
GAGCGGGAGGCTGCGGCCGAGTTAGAGGCTGCTCGGGGCCGTTTTGCAGAGGCTGAGGAGGCGGCGCGGG
GGCGGAGCCGCGAGCTAGAGGCGCTTCGGGAATTGCTGGCCACGGCCACGGCTACCGGCGAGCGCGCACG
CACAGAGGCCGCCGAGTTGCGCCAAGCGCTAGCTGCCTCCGAGGCTCGGGTCGCCGAACTCAGCTCTACC
GTGGACGCCGCTCGCGAGGAGCTGGAGCGCATGCGCGGGGCCTCGGTTCCTGCGGACGAGCACGAGCACG
CGCTGAGCGCCCTGCGCGACCACGTGACCCGGCTGCAGGCGCAGCTGGCGGACCTGGCGCGCAGGCACGA
GAAGACTAGCGCAGAGGTCTTCCAGATCACAGACCTGTCCAAAGAAGTCTTTACACTGAAGGAGGCTCTG
AAGGTGCAGCAGTCCACCCCAGCCAGCTCCAAGGAGGAGGAGGCTCTGCGCGGCCAGGTGACAGCCCTGC
AGCAGCAGATACAGGAGGAGGCCCGGGAGCATGGCGCTGTGGTGGCTTTATACCGCACCCATCTCCTGTA
TGCCATTCAGGGACAGATGGATGAGGATGTACAGTGCATTTTGAGCCAGATTCTGCAGATGCAGCGGCTT
CAGGCTCAGGGTCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211137 representing NM_027480
Red=Cloning site Green=Tags(s)

MKTLRARFKKTEGQDWGKSDQRLLQAVENNDVARVASLIAHKGLVPTKLDPEGKSAFHLAAMRGAAGCLE
VMLAQGADVMSTDGAGYNALHLAAKYGHPECLKQLLEASCVVDIEDSSGWTALHHAAAGGCLSCSKLLCS
FKAHMNPRDRSGATPLIIAAQMCHTDLCRLLLQQGAATNDQDLQGRTALMLACEGGSPETVEVLLQGGAQ
LSITDALGQDATHYGALTGDKLILQLLHESARRSSPPSASLEEDSGEASSQNSVSSHEKQGAPKKRKAPQ
PPASTPVPDDRDAYEEIVRLRQERGRLLQKIRGLEQHKERRRKEPLEAEASSVHSLERQVQELQQMLAEK
QEEKESLGREVESLQSRLSLLENERENTSYDVATLQDEEGEMPDFPGADALMPKNQSPSAEEIVASLQEQ
VAQLTRQNQELLEKVQILEEFEKDEAQMAEESQAEVVPLVLYESLRAELEQLRRQYTEAMHSQQQQQEGE
PPRAQEGEETAYQEIKDKGITIQNGPSVPDLNGTTYAETKANGMELQAGGSKGVWNTEAGVSEAAPIEPE
AAGSEATGKDRLAAKEMDTSATMAEALNVKALGDNAESEPVAAEDTGGKENPGMKADEVDVLAQAGLTGT
VIRNMEAIGVRDTGIQATGLEAKAVKTTGVQATVAEVIGVKVTGVQTTAIEAIGVKDTTQVATGAQADCW
QATEADCTGAQDTAMEPTGAQATVTETTEAETSGTEDPCAAILHPGAAAAALQAELETRIRGLEEALRRR
EREAAAELEAARGRFAEAEEAARGRSRELEALRELLATATATGERARTEAAELRQALAASEARVAELSST
VDAAREELERMRGASVPADEHEHALSALRDHVTRLQAQLADLARRHEKTSAEVFQITDLSKEVFTLKEAL
KVQQSTPASSKEEEALRGQVTALQQQIQEEAREHGAVVALYRTHLLYAIQGQMDEDVQCILSQILQMQRL
QAQGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027480
ORF Size 2955 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027480.3, NP_081756.4
RefSeq Size 3658 bp
RefSeq ORF 2958 bp
Locus ID 70615
UniProt ID Q80VM7
Cytogenetics 10 39.72 cM
MW 106.2 kDa
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Documents
Resources
"NM_027480" in other vectors (4)
SKU Description Size Price
MC205917 Ankrd24 (untagged) - Mouse ankyrin repeat domain 24 (Ankrd24), (10ug) 10 ug
$903.00
MG211137 Ankrd24 (tGFP-tagged) - Mouse ankyrin repeat domain 24 (Ankrd24) 10 ug
$1,102.00
MR211137L3 Lenti ORF clone of Ankrd24 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 24 (Ankrd24) 10 ug
$1,202.00
MR211137L4 Lenti ORF clone of Ankrd24 (mGFP-tagged) - Mouse ankyrin repeat domain 24 (Ankrd24) 10 ug
$1,202.00

Plasmid Map for MR211137

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211137
Circular map for MR211137

file-alt Citations

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