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Copb2 (NM_015827) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211128
Copb2 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 2 (beta prime) (Copb2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $829.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Copb2 or NM_015827"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Copb2
Synonyms AI256832
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211128 representing NM_015827
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCTTCGACTTGACATCAAGAGAAAGCTGACTGCTAGGTCTGACCGAGTTAAGAGCGTGGATTTGC
ATCCCACAGAGCCATGGATGTTGGCTAGTCTTTACAATGGCAGTGTGTGTGTTTGGAATCATGAAACTCA
GACACTGGTGAAGACGTTTGAAGTATGTGATCTTCCTGTTCGAGCTGCAAAGTTTGTCGCAAGGAAGAAC
TGGGTGGTGACAGGAGCAGACGATATGCAGATCAGAGTGTTCAATTATAACACCCTGGAGAGGGTGCATA
TGTTTGAAGCACACTCGGATTACATTCGTTGTATTGCTGTTCATCCCACCCAGCCGTTCATTCTCACCAG
CAGTGATGACATGCTTATTAAGCTCTGGGACTGGGATAAAAAATGGTCTTGTTCACAAGTGTTTGAAGGA
CACACGCACTACGTTATGCAGATTGTGATAAACCCCAAAGATAACAATCAGTTTGCCAGTGCATCTCTCG
ACAGGACCATCAAGGTGTGGCAGCTGGGCTCCTCATCACCAAACTTCACTCTGGAAGGACATGAAAAAGG
CGTGAATTGCATTGACTACTACAGTGGTGGTGACAAGCCATACCTCATTTCAGGTGCAGATGACCGGCTT
GTTAAAATATGGGATTATCAGAACAAAACGTGTGTGCAGACCCTGGAGGGGCATGCCCAGAATGTGTCGT
GTGCGAGCTTCCATCCTGAGCTGCCCATCATCATCACAGGGTCAGAAGACGGAACTGTGCGAATCTGGCA
TTCTAGCACCTACCGGCTTGAGAGCACGCTGAATTACGGAATGGAGAGGGTGTGGTGTGTGGCCAGTCTG
AGAGGGTCCAACAATGTCGCTCTGGGCTATGATGAAGGGAGCATCATTGTTAAGCTTGGTCGGGAGGAAC
CTGCCATGTCCATGGATGCCAATGGAAAGATAATCTGGGCCAAGCATTCAGAAGTCCAGCAGGCCAACCT
GAAAGCAATGGGTGACACTGAGATTAAAGATGGAGAAAGATTGCCACTGGCAGTAAAGGACATGGGCAGT
TGTGAAATATATCCACAGACCATTCAGCACAATCCTAATGGGAGGTTTGTTGTGGTATGTGGTGATGGGG
AGTATATCATCTACACAGCAATGGCATTGAGGAACAAGAGCTTCGGGTCTGCCCAGGAGTTTGCATGGGC
CCACGATTCTTCAGAGTATGCAATAAGAGAAAGCAACAGTATTGTAAAGATATTTAAAAACTTTAAGGAA
AAAAAATCATTTAAACCCGATTTTGGAGCTGAAAGTATCTATGGGGGCTTCTTGCTGGGAGTCAGATCTG
TAAATGGCTTAGCCTTCTATGACTGGGAGAATACTGAACTCATCCGCAGAATTGAAATTCAGCCCAAGCA
CATCTTCTGGTCTGACTCTGGAGAGCTGGTCTGTATCGCCACCGAGGAGTCATTTTTTATTCTTAAATAT
CTGTCAGAAAAAGTCTTGGCTGCACAGGAAACACATGAGGGAGTTACTGAAGATGGCATTGAAGACGCCT
TTGAGGTTCTTGGTGAAATTCAGGAAATTGTGAAAACTGGGCTGTGGGTAGGTGACTGCTTCATTTACAC
AAGTTCTGTGAACAGATTAAACTATTACGTGGGAGGAGAGATAGTCACCATCGCCCACCTGGACAGGACA
ATGTATCTTCTGGGCTATATTCCAAAAGACAATAGACTTTATCTGGGGGATAAGGAATTGAATATTGTTA
GCTACTCCCTGTTGGTTTCAGTCCTGGAATACCAGACTGCTGTCATGCGGAGGGATTTTAGCATGGCGGA
TAAAGTCCTCCCTACCATCCCCAAAGAACAGAGGACCAGAGTTGCACACTTTTTGGAAAAACAGGGCTTC
AAGCAGCAAGCACTTACAGTATCCACAGATCCTGAACATCGTTTTGAGCTTGCTCTTCAACTTGGAGAGT
TGAAAATTGCTTACCAGTTAGCAGTGGAAGCAGAGTCGGAGCAGAAGTGGAAACAACTTGCTGAACTTGC
CATTAGTAAATGTCAGTTCAGCCTAGCCCAGGAGTGTCTGCACCATGCTCAGGATTATGGTGGTCTGCTG
CTCTTGGCCACTGCTTCTGGAAATGCTAGTATGGTGAACAAGCTGGCAGAAGGTGCGGAGAGAGACGGCA
AAAATAACGTGGCATTCATGAGCTATTTTTTACAGGGCAAGCTTGATGCTTGCCTAGAGCTCTTGATCAG
AACAGGAAGACTCCCAGAAGCTGCCTTCCTGGCCCGAACTTACTTACCCAGTCAGGTCTCAAGGGTGGTG
AAACTGTGGAGGGAGAATCTCTCAAAAGTCAACCAGAAAGCAGCAGAATCCCTTGCTGATCCTACAGAGT
ATGAAAACCTTTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAGAATGGGTGAAGGAGACACATGCGGA
TTTGTGGCCAGCCAAGCAGTACCCTCTTGTCACGCCAAATGAAGAGAGAAATGTTATGGAAGAGGCAAAA
GGCTTTCAGCCCTCAAGACCCACAGCCCAGCAGGAACCTGATGGAAAACCTGCTTCTAGCCCAGTTATCA
TGGCCTCCCAAACTACCCACAAAGAAGAAAAGAGTTTACTAGAACTAGAAGTTGATTTGGATAACTTGGA
ATTAGAGGATATTGACACAACAGACATTAATCTGGATGAAGATATTCTGGATGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211128 representing NM_015827
Red=Cloning site Green=Tags(s)

MPLRLDIKRKLTARSDRVKSVDLHPTEPWMLASLYNGSVCVWNHETQTLVKTFEVCDLPVRAAKFVARKN
WVVTGADDMQIRVFNYNTLERVHMFEAHSDYIRCIAVHPTQPFILTSSDDMLIKLWDWDKKWSCSQVFEG
HTHYVMQIVINPKDNNQFASASLDRTIKVWQLGSSSPNFTLEGHEKGVNCIDYYSGGDKPYLISGADDRL
VKIWDYQNKTCVQTLEGHAQNVSCASFHPELPIIITGSEDGTVRIWHSSTYRLESTLNYGMERVWCVASL
RGSNNVALGYDEGSIIVKLGREEPAMSMDANGKIIWAKHSEVQQANLKAMGDTEIKDGERLPLAVKDMGS
CEIYPQTIQHNPNGRFVVVCGDGEYIIYTAMALRNKSFGSAQEFAWAHDSSEYAIRESNSIVKIFKNFKE
KKSFKPDFGAESIYGGFLLGVRSVNGLAFYDWENTELIRRIEIQPKHIFWSDSGELVCIATEESFFILKY
LSEKVLAAQETHEGVTEDGIEDAFEVLGEIQEIVKTGLWVGDCFIYTSSVNRLNYYVGGEIVTIAHLDRT
MYLLGYIPKDNRLYLGDKELNIVSYSLLVSVLEYQTAVMRRDFSMADKVLPTIPKEQRTRVAHFLEKQGF
KQQALTVSTDPEHRFELALQLGELKIAYQLAVEAESEQKWKQLAELAISKCQFSLAQECLHHAQDYGGLL
LLATASGNASMVNKLAEGAERDGKNNVAFMSYFLQGKLDACLELLIRTGRLPEAAFLARTYLPSQVSRVV
KLWRENLSKVNQKAAESLADPTEYENLFPGLKEAFVVEEWVKETHADLWPAKQYPLVTPNEERNVMEEAK
GFQPSRPTAQQEPDGKPASSPVIMASQTTHKEEKSLLELEVDLDNLELEDIDTTDINLDEDILDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015827
ORF Size 2715 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015827.2, NP_056642.1
RefSeq Size 3043 bp
RefSeq ORF 2718 bp
Locus ID 50797
UniProt ID O55029
Cytogenetics 9 E3.3
MW 102.9 kDa
Summary The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_015827" in other vectors (4)
SKU Description Size Price
MC205628 Copb2 (untagged) - Mouse coatomer protein complex, subunit beta 2 (beta prime) (Copb2), (10ug) 10 ug
$829.00
MG211128 Copb2 (tGFP-tagged) - Mouse coatomer protein complex, subunit beta 2 (beta prime) (Copb2) 10 ug
$789.00 $1,029.00
MR211128L3 Lenti ORF clone of Copb2 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 2 (beta prime) (Copb2) 10 ug
$1,129.00
MR211128L4 Lenti ORF clone of Copb2 (mGFP-tagged) - Mouse coatomer protein complex, subunit beta 2 (beta prime) (Copb2) 10 ug
$1,129.00

Plasmid Map for MR211128

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211128
Circular map for MR211128

file-alt Citations

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