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Aftph (NM_181411) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211121
Aftph (Myc-DDK-tagged) - Mouse aftiphilin (Aftph)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $828.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aftph
Synonyms 9130023F12Rik; AU015099; AU016543; AU018432; AU019184; AU044244; RP23-452C23.3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211121 representing NM_181411
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCAGACATCATTCGAATGTACTCTTCCTCCCCCCCACCTCTAGACAACGGAGCAGAGGATGATG
AGGAGGATGAGTTTGGGGAGTTTGGTGGGTTTTCGGAAGTCAGCCCTTCTGGTGTAGGGTTTGTTGATTT
TGACACACCAGATTATACTCGTCCCAAGGAAGACTTTGTACCTTCAAACCACTTTATGCCAATTCATGAA
TATTCAGAAGATGTAGATAGCCTTACAAGCTTTAAGTCTGTTCAGAATGGTAATGATAAGGATATCACTG
CTGAACTTTCTACTCCTGTGAAAAGTCAATCTGATGTTGTACTTTCTACCACCAGCAAAGAAATGATTCC
ATCTAAAACTTTGGATCCTTCCATCGATGGTATGGAAAGCCTGGAAGACTTAGATAAAGTAGTGGTGCAG
GGACCAAGCACTGGGCAACTCAGAAGTTTCTCTCCTGGAGATTTTAGAACTGATAAGAACATTGTTCACC
AAACCAAGCAGTTAGAGAGCTGCAACGGTGAAAAGCCTCCTTGTCTGGAAATTCTAACAAACGGGTTTGC
AGGTTTGGAAACTGTAAATCCTCAGGGAACAGATGACCTGGACAATGTGGCAGATTCAAAGGGATCGAAG
CCTCTTAACACTTGTGGCACCGAGTGCATTTTAGAGTCTGCAGCTAGTCATGCTACGGAATTTGCAGATT
TTTCTACATTTTCCCAAACAGAAAGGACACAACTGGAAGAAATAGAATGTCCAGTTTTAAATGATGGTGA
TACACTGACCATTCAGGGAAACAGCAAAGGCCCCAGAGTCAAGGAACTAAACTGTGTGAAAGAAGTGACT
TTGGATGGAAGCTTTGAGGATACGGGGAACACTGAAAGAGAACATCAGGTCTGCGTCTCAGAAATCCATG
CCGTAGCTGACAGAGGGCTCAGTGTTGAAAAACAAGACCTTCAAACACTGCAACAGGATGAGTTTCTAAA
TTCAAGGATTCAGTCTGAGGCTTGGAGCTTGGTAGACTCAAGTGAAAATTCAGAAGCCATTACAAAGGAG
CGGTGTAAAATGGAAAAAAATGACTTATTTGCCTCTAAGTGTGCTGACTTATCCATGGATTCTGTTAAAA
CTTCAGACGTTAATGAAATTGGTTCTTCCAAAGAAGAAAACAGAAAGCTGACTAATCCCAAAAGCCCAGA
CCCTGATCCCACAGGACAAAATGCTTTGGATGATTCTGCTGCAAGCATGAAAAATGGTGACAGTGGGAAT
GGCTTTGTGACTTGTCATGATACCAATGAGGATGATTTTGGTGACTTTGGTACAGCCAATGGCACCACTC
CACCCTTTGTTACTAGTACTCAAGATTCAATGAGTGACGTCACTTTTGAAGACTCCTCAGAGCATTTTCT
ACATCTTAGTGAACCAGGTGATGATTTTGGAGAATTTGAGGATACAAATGCTGTTTCTTGTCAAGAGGAA
ATGAGATTTACTGAGTCAGACCTAAGACAGACTTCTGATGGCTTATCAGAGGAATGTCCATTGGCAGGAG
AGTCTGGTGGAAAAGACAGTAAACCTGACTCAAAACTGAAAAATGGTCAAGACAGTGAATTTGGAGATTT
TGATTCTGTGCCAAATACTCAAGGTAGTGCTTTTCAAGACTCGGATGATTTTGCAGACTTCAGTTCAGCA
GGTCCTAGCCAAGCTGTAGATTGGAATGCTTTTGAGGATGAACAGAAAGATGGTTGTTCTTGGGCTGCCT
TTGGGGATCAGCAGGAGACTGAGTCTCATCATCTAAAGGAAGTCTGGCAGTCACAGAGGACAGATGAAAC
TATGGGCACTCTGGGAACCCCTAAGATGCACAGTGTTTCTTCAGCAGCTTCCAAAGGAGCAGTTGCTAGT
GGCCATTTGCAGGAACCAGGCACTTCAGTTCAGACAGCTTTACTCAACCGGTTGGAGAGAATTTTCGAAG
CCTGTTTTCCGTCTGTGTTTGTCCCTGATGTGGAAGAGGAGGTTTCTTCCCTGAAGCACTTGCTGGAGAC
ACACAGCTCGCCAGCAAAAACAAGAGAGGCCTTAGCTGACCGTGGTGAGTTGAGGGGTGTATGGACCGAA
CTGCAGGACATCCATGATGCACATGGTTTGCGGTACCAGTGGGGTGGCTCACATAGCAACAAGAAGCTTT
TGTGCTCCTTGGGAATAGACACTCGAAACATTCTATTCACTGGCAATAAGAAGCAACCTGTTATAGTGCC
CATGTATGCAGCAGGATTGGGAATGTTAGAGCCCACCAAGGAACCATTAAAACCACTATCTGCTGCAGAA
AAAATTGCTTCTATTGGTCAGACAACCGTTATGACACCAGAAATAAACACGTGTACATCTGATCCATTCC
AGGAGTCTCTCCCACCTGTCCAGTTTGACTGGAGTAGCAGTGGCCTTACTAACCCTTTAGATGGTGTGGA
TCCAGAGTTGTATGAGTTAACAACTGCTAAGCTGGAGACCTCCACCTCAAGCCTCAGAGTGACTGATGCA
TTTGCGAAGCTCATGTCTACAGTGGAAAAGACAAGCACGTCAACCAGGAAACCAAAAAGAGAGGAGCACC
TGAGTGAGGAAGCCATGAAGGTGATCGCCAGCCTTCCAGACCTGACCTTCATGCATGCCAAGGTGTTGAT
GTTCCCAGCCACTCTGACACCTTCCATGAGCTCCCAAGAACAAGCAGACGCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211121 representing NM_181411
Red=Cloning site Green=Tags(s)

MEPDIIRMYSSSPPPLDNGAEDDEEDEFGEFGGFSEVSPSGVGFVDFDTPDYTRPKEDFVPSNHFMPIHE
YSEDVDSLTSFKSVQNGNDKDITAELSTPVKSQSDVVLSTTSKEMIPSKTLDPSIDGMESLEDLDKVVVQ
GPSTGQLRSFSPGDFRTDKNIVHQTKQLESCNGEKPPCLEILTNGFAGLETVNPQGTDDLDNVADSKGSK
PLNTCGTECILESAASHATEFADFSTFSQTERTQLEEIECPVLNDGDTLTIQGNSKGPRVKELNCVKEVT
LDGSFEDTGNTEREHQVCVSEIHAVADRGLSVEKQDLQTLQQDEFLNSRIQSEAWSLVDSSENSEAITKE
RCKMEKNDLFASKCADLSMDSVKTSDVNEIGSSKEENRKLTNPKSPDPDPTGQNALDDSAASMKNGDSGN
GFVTCHDTNEDDFGDFGTANGTTPPFVTSTQDSMSDVTFEDSSEHFLHLSEPGDDFGEFEDTNAVSCQEE
MRFTESDLRQTSDGLSEECPLAGESGGKDSKPDSKLKNGQDSEFGDFDSVPNTQGSAFQDSDDFADFSSA
GPSQAVDWNAFEDEQKDGCSWAAFGDQQETESHHLKEVWQSQRTDETMGTLGTPKMHSVSSAASKGAVAS
GHLQEPGTSVQTALLNRLERIFEACFPSVFVPDVEEEVSSLKHLLETHSSPAKTREALADRGELRGVWTE
LQDIHDAHGLRYQWGGSHSNKKLLCSLGIDTRNILFTGNKKQPVIVPMYAAGLGMLEPTKEPLKPLSAAE
KIASIGQTTVMTPEINTCTSDPFQESLPPVQFDWSSSGLTNPLDGVDPELYELTTAKLETSTSSLRVTDA
FAKLMSTVEKTSTSTRKPKREEHLSEEAMKVIASLPDLTFMHAKVLMFPATLTPSMSSQEQADA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181411
ORF Size 2712 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181411.4, NP_852076.1
RefSeq Size 3984 bp
RefSeq ORF 2715 bp
Locus ID 216549
UniProt ID Q80WT5
Cytogenetics 11 A3.1
MW 98.5 kDa
Summary May play a role in membrane trafficking.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_181411" in other vectors (4)
SKU Description Size Price
MC203626 Aftph (untagged) - Mouse aftiphilin (Aftph), (10ug) 10 ug
$829.00
MG211121 Aftph (tGFP-tagged) - Mouse aftiphilin (Aftph) 10 ug
$1,028.00
MR211121L3 Lenti ORF clone of Aftph (Myc-DDK-tagged) - Mouse aftiphilin (Aftph) 10 ug
$1,128.00
MR211121L4 Lenti ORF clone of Aftph (mGFP-tagged) - Mouse aftiphilin (Aftph) 10 ug
$1,128.00

Plasmid Map for MR211121

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211121
Circular map for MR211121

file-alt Citations

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