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Ect2 (NM_001177626) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211053
Ect2 (Myc-DDK-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 3
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $808.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ect2
Synonyms AI528536; mKIAA4037
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211053 representing NM_001177626
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACGACAGCGTGTTACCATCTCCTTCTGAGATAACTAGCTTGGCAGACTCTTCAGTGTTTGATT
CTAAAGTTGCTGAAATGTCCAAGGAAAACTTGTGCTTGGCGTCTACTTCAAATGTTGATGAAGAAATGCC
GCAGGTTGAAGCAAGAGTGATAATGGTCCAGGATGCTGGGAAACAAGAAGAACTTCTAAAGGCCTTAAAG
GAAATGAAAGTGCCCTGTGTAAAGATGGACTCAATGGAAGAATTTGAAAGTTTGGATTCCCCAGAATTTG
AGAATATATTTGTAGTTACTGACTTCCAGAATTCTGTCTTCAATGACTTATACAAGGCTGATTGTAGGAT
TGTTGGGCCACCAGTTATACTCAACTGTGCACAAAGGGGAGAGCCCTTGCCGTTTTCCTGCCGGCCGCTG
TATTGTACGAGTATGCTGAACCTGGTGCTCTGCTTCACTGGATTCAGGAAGAAGGAGGAGCTTGTCAAAT
TGGTGACGTTGGTTCATCATATGGGTGGAGTTATTCGAAAAGAGTGTAATTCCAAAGTAACACATCTGGT
GGCAAATTGTACACAAGGCGAAAAATTTAGGGTTGCTGTGAGCCTGGGCACTCCAATTATGAAGCCAGAA
TGGATTTATAAAGCGTGGGAAAGACGCAATGAACAGTGTTTCTGTGCAGCAGTTGATGACTTTAGAAATG
AATTTAAAGTTCCTCCATTCCAAGATTGCATTTTAAGTTTCCTGGGATTTTCAGATGAAGAGAAACATAG
TATGGAAGAAATGACTGAAATGCAAGGAGGTAGCTATTTACCAGTTGGGGATGAAAGGTGCACTCACCTT
ATTGTTGAAGAGAATACAGTAAAGGACCTTCCATTCGAACCTTCAAAGAAACTTTTTGTTGTCAAGCAAG
AGTGGTTCTGGGGAAGCATTCAGATGGACGCTCGTGCAGGAGAGACTATGTATTTGTATGAAAAGGCTAA
TACTCCTGAGCTCAAGAAATCGGTGTCTCTGCTTTCTCTAAGTACTCCAAACAGCAACCGCAAGAGACGG
AGATTGAAAGAGACCCTGGCTCAGCTCTCCAGGGAGACTGACCTCTCTCCTTTCCCTCCCCGGAAGCGGC
CCTCAGCTGAGCACTCTCTTTCTATTGGCTCACTCCTGGATATCTCCAACACACCCGAGTCCAGCATCCA
CTATGGAGAAACACCGAAGTCCTGTGCTAAGTCTTCTAGAAGTTCTACTCCAGTTCCTCCAAAGCAGTCA
GCCAGGTGGCAGGTGGCGAAAGAGCTCTACCAGACTGAGAGCAATTATGTCAATATACTGGCCACAATCA
TTCAATTATTTCAGGTGCCCTTAGAAGAAGAAGGACAGCGTGGTGGGCCTATTCTTGCACCTGAGGAGAT
CAAGACTATTTTTGGGAGCATCCCAGATATCTTTGATGTGCATATGAAGATCAAGGATGATCTTGAAGAC
CTTATTGCTAACTGGGATGAGAGCAGAAGTATTGGTGACATCTTTCTTAAATATGCAAAAGATTTGGTAA
AAACCTACCCTCCGTTTGTAAACTTCTTTGAAATGAGCAAGGAAATGATCATTAAATGTGAGAAACAAAA
GCCCAGATTTCATGCTTTCCTCAAGATAAATCAAGCTAAACCAGAATGTGGACGACAGAGCCTTGTGGAA
CTTCTCATCCGGCCAGTGCAGAGGCTACCCAGTGTCGCTTTACTTTTAAACGATCTTAAAAAGCATACAG
CTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGATCACTAAAAGAAGTAATGACACATAT
CAATGAGGATAAGAGAAAAACAGAAGCACAGAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGC
CCAGCTAATCTCTTATCTTCTCATCGGAGCCTGGTGCAACGAGTGGAAACGGTTTCCCTTGGTGAGCACC
CTTGCGACCGAGGAGAACAAGTCACTCTCTTCCTCTTCAATGACTGCCTCGAGATAGCAAGAAAGCGGCA
CAAGGTTATTGGCACTTTTAGAAGTCCTCACGACCGCACCCGGCCCCCCGCTTCTCTGAAGCACATTCAT
CTCATGCCTCTTTCTCAGATTAAGAAGGTGCTGGACATCCGAGAGACAGAAGATTGTCACAATGCCTTTG
CCTTGCTTGTAAGGCCACCAACAGAACAGGCCAATGTACTGCTCAGCTTCCAGATGACGTCAGAGGAGCT
TCCAAAGGAGAGCTGGCTGAAGATGCTGTGCCGACATGTAGCCAACACCATTTGTAAGGCAGATGCTGAG
AATCTTATGTATGTGGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGATAGTACATTGAGTC
GAGCATCTAGAGCAATAAAGAAGACTTCAAAAAAGGTTACAAGGGCATTCTCTTTTTCCAAAACTCCCAA
GAGAGCCCTGCGGATGGCTCTTTCATCATCCCATAGCTCAGAGGGAAGGAGTCCTCCGAGCAGTGGCAAG
CTTGCTGTGAGCCGTCTGTCCAGCACATCATCCTTAGCAGGTATTCCATCTCCCTCCCTTGTCAGCCTCC
CTTCTTTCTTTGAACGGAGAAGTCATACATTAAGTCGATCTACAACTCACTTGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211053 representing NM_001177626
Red=Cloning site Green=Tags(s)

MADDSVLPSPSEITSLADSSVFDSKVAEMSKENLCLASTSNVDEEMPQVEARVIMVQDAGKQEELLKALK
EMKVPCVKMDSMEEFESLDSPEFENIFVVTDFQNSVFNDLYKADCRIVGPPVILNCAQRGEPLPFSCRPL
YCTSMLNLVLCFTGFRKKEELVKLVTLVHHMGGVIRKECNSKVTHLVANCTQGEKFRVAVSLGTPIMKPE
WIYKAWERRNEQCFCAAVDDFRNEFKVPPFQDCILSFLGFSDEEKHSMEEMTEMQGGSYLPVGDERCTHL
IVEENTVKDLPFEPSKKLFVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSLLSLSTPNSNRKRR
RLKETLAQLSRETDLSPFPPRKRPSAEHSLSIGSLLDISNTPESSIHYGETPKSCAKSSRSSTPVPPKQS
ARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHMKIKDDLED
LIANWDESRSIGDIFLKYAKDLVKTYPPFVNFFEMSKEMIIKCEKQKPRFHAFLKINQAKPECGRQSLVE
LLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGC
PANLLSSHRSLVQRVETVSLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHDRTRPPASLKHIH
LMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSEELPKESWLKMLCRHVANTICKADAE
NLMYVADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRMALSSSHSSEGRSPPSSGK
LAVSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001177626
ORF Size 2646 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001177626.1, NP_001171097.1
RefSeq Size 4040 bp
RefSeq ORF 2649 bp
Locus ID 13605
UniProt ID Q07139
Cytogenetics 3 A3
MW 100.1 kDa
Summary Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001177626" in other vectors (3)
SKU Description Size Price
MC220714 Ect2 (untagged) - Mouse ect2 oncogene (Ect2), transcript variant 3, (10ug) 10 ug
$889.00
MR211053L3 Lenti ORF clone of Ect2 (Myc-DDK-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 3 10 ug
$1,108.00
MR211053L4 Lenti ORF clone of Ect2 (mGFP-tagged) - Mouse ect2 oncogene (Ect2), transcript variant 3 10 ug
$1,108.00

Plasmid Map for MR211053

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211053
Circular map for MR211053

file-alt Citations

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