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Dis3l2 (NM_153530) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211017
Dis3l2 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $797.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dis3l2
Synonyms 4930429A22Rik; 8030493P09Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211017 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCATCCTGACTACAAGCTGAACCTTCGGTCTCCGGGGACCCCCAGAGGTGTGTCCTCTGTGGTTG
GCCCGAGTGCTGTTGGTGCTTCGCCAGGTGACAAAAAGTCAAAGAACAAGTCCATGCGAGGGAAGAAAAA
GAGCATATTTGAAACCTACATGTCCAAGGAGGATGTTTCAGAAGGCTTGAAGAGAGGAACACTTATCCAG
GGTGTATTGAGAATCAACCCAAAGAAGTTTCATGAAGCCTTCATTCCTTCTCCGGATGGTGATCGGGACA
TTTTTATTGATGGAGTTGTTGCTCGTAATAGAGCCTTAAATGGGGACCTTGTGGTTGTAAAACTGCTTCC
TGAGGATCAGTGGAAGGCAGTTAAACCAGAGAGCAATGACAAAGAAATAGAAGCTACTTATGAAGCTGAC
ATCCCTGAAGAGGGCTGTGGACATCACCCCCTGCAGCAGTCCCGGAAAGGCTGGAGTGGTCCTGATGTCA
TTATAGAGGCTCAGTTTGATGACAGCGACTCAGAAGATAGACATGGCAACACCAGTGGCCTGGTTGATGG
TGTTAAGAAATTGTCAATCTCTACTCCTGACAGAGGAAAAGAAGATTCTAGTACTCCAGTTATGAAAGAT
GAGAACACCCCCATACCACAGGACACAAGAGGCTTATCAGAGAAGTCACTTCAGAAATCAGCAAAGGTGG
TTTACATCTTGGAGAAAAAGCATTCTCGAGCAGCAACTGGCATCCTGAAACTCTTGGCTGATAAGAACAG
TGACCTGTTTAAGAAATACGCCCTGTTTTCTCCTTCAGACCACCGAGTACCTAGAATTTACGTACCTCTC
AAGGACTGTCCCCAGGACTTCATGACCCGACCTAAAGACTTTGCCAACACGCTGTTCATCTGCCGCATCA
TAGACTGGAAGGAGGACTGTAATTTTGCCCTGGGGCAACTGGCTAAGAGTCTTGGGCAGGCTGGTGAAAT
CGAGCCTGAAACAGAAGGGATACTGACAGAATATGGTGTGGACTTCTCTGATTTCTCTTCAGAAGTTCTT
GAATGTCTCCCTCAAAGCCTGCCCTGGACAATCCCACCTGATGAGGTGGGCAAGAGAAGAGACCTAAGGA
AAGACTGTATCTTCACCATTGATCCATCAACTGCTCGCGACCTTGATGATGCCCTCGCCTGCAGGCGGCT
CACTGATGGCACCTTCGAAGTGGGCGTCCACATCGCCGATGTGAGTTACTTTGTTCCTGAGGGATCCTCT
TTGGATAAAGTAGCTGCTGAGAGAGCCACAAGTGTCTACTTGGTCCAGAAGGTGGTCCCCATGCTTCCCA
GGCTTCTGTGTGAGGAACTCTGCAGCCTCAACCCCATGACTGACAAGCTGACCTTCTCTGTGATCTGGAA
GCTGACCCCTGAAGGCAAGATCCTTGAAGAGTGGTTTGGCCGCACTATCATCCGTTCTTGCACCAAACTG
AGCTACGACCATGCCCAGAGCATGATCGAAAATCCAACTGAGAAGATCCCTGAGGAAGAGCTTCCCCCAA
TTTCTCCAGAGCACAGCGTCGAGGAGGTGCACCAGGCAGTCCTGAACCTGCACAGCATTGCAAAGCAACT
CCGCCGCCAGCGCTTTGTAGATGGCGCACTCCGTTTAGATCAGCTGAAGCTTGCTTTTACTCTGGACCAT
GAGACTGGATTGCCTCAAGGATGTCACATCTATGAGTACCGAGACAGCAACAAGCTTGTAGAGGAGTTCA
TGCTCCTGGCCAACATGGCGGTGGCCCACAAGATCTTCCGCACCTTCCCTGAGCAGGCCCTGCTGCGCCG
GCATCCCCCACCACAGACGAAGATGCTCAGTGACCTGGTGGAGTTCTGTGACCAGATGGGGCTGCCCATG
GATGTCAGCTCTGCAGGGGCCCTAAATAAAAGCCTGACTAAGACATTTGGAGATGACAAGTACTCTCTGG
CCCGGAAGGAGGTGCTCACCAACATGTACTCCCGGCCCATGCAGATGGCACTGTACTTCTGCTCTGGGAT
GCTGCAGGACCAGGAGCAGTTCCGGCATTATGCTCTCAACGTTCCCCTCTACACACACTTCACCTCTCCC
ATCCGCCGCTTTGCTGACGTCATAGTGCACCGCCTCCTGGCTGCTGCTCTGGGCTACAGTGAACAGCCAG
ATGTGGAGCCTGATACCCTACAGAAGCAAGCTGACCACTGCAATGACCGTCGCATGGCTTCCAAACGTGT
GCAGGAGCTCAGCATCGGCCTCTTCTTCGCAGTTCTAGTAAAGGAGAGTGGCCCCCTGGAGTCCGAAGCC
ATGGTGATGGGTGTCCTGAACCAAGCTTTCGACGTGCTGGTGCTGCGCTTTGGGGTGCAGAAGCGCATCT
ACTGCAATGCACTGGCCCTGCGATCCTACAGCTTCCAGAAGGTGGGGAAGAAGCCAGAGCTCACTCTTGT
TTGGGAGCCTGATGACCTTGAAGAGGAGCCAACACAGCAGGTCATCACCATCTTCAGCCTGGTGGATGTG
GTCCTGCAGGCAGAGGCCACAGCCCTCAAGTACAGTGCTATCCTGAAGCGACCAGGCCTGGAGAAGGCGT
CTGATGAGGAGCCTGAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211017 protein sequence
Red=Cloning site Green=Tags(s)

MNHPDYKLNLRSPGTPRGVSSVVGPSAVGASPGDKKSKNKSMRGKKKSIFETYMSKEDVSEGLKRGTLIQ
GVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEDQWKAVKPESNDKEIEATYEAD
IPEEGCGHHPLQQSRKGWSGPDVIIEAQFDDSDSEDRHGNTSGLVDGVKKLSISTPDRGKEDSSTPVMKD
ENTPIPQDTRGLSEKSLQKSAKVVYILEKKHSRAATGILKLLADKNSDLFKKYALFSPSDHRVPRIYVPL
KDCPQDFMTRPKDFANTLFICRIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILTEYGVDFSDFSSEVL
ECLPQSLPWTIPPDEVGKRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFEVGVHIADVSYFVPEGSS
LDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILEEWFGRTIIRSCTKL
SYDHAQSMIENPTEKIPEEELPPISPEHSVEEVHQAVLNLHSIAKQLRRQRFVDGALRLDQLKLAFTLDH
ETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRTFPEQALLRRHPPPQTKMLSDLVEFCDQMGLPM
DVSSAGALNKSLTKTFGDDKYSLARKEVLTNMYSRPMQMALYFCSGMLQDQEQFRHYALNVPLYTHFTSP
IRRFADVIVHRLLAAALGYSEQPDVEPDTLQKQADHCNDRRMASKRVQELSIGLFFAVLVKESGPLESEA
MVMGVLNQAFDVLVLRFGVQKRIYCNALALRSYSFQKVGKKPELTLVWEPDDLEEEPTQQVITIFSLVDV
VLQAEATALKYSAILKRPGLEKASDEEPED

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153530
ORF Size 2610 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153530.1, NP_705758.1
RefSeq Size 3129 bp
RefSeq ORF 2613 bp
Locus ID 208718
UniProt ID Q8CI75
Cytogenetics 1 C5
MW 97.8 kDa
Summary 3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_153530" in other vectors (5)
SKU Description Size Price
MC201819 Dis3l2 (untagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2, (10ug) 10 ug
$797.00
MC201820 Dis3l2 (untagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2, (10ug) 10 ug
$797.00
MG211017 Dis3l2 (tGFP-tagged) - Mouse RIKEN cDNA 4930429A22 gene (4930429A22Rik) 10 ug
$997.00
MR211017L3 Lenti ORF clone of Dis3l2 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2 10 ug
$1,097.00
MR211017L4 Lenti ORF clone of Dis3l2 (mGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 2 10 ug
$1,097.00

Plasmid Map for MR211017

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211017
Circular map for MR211017

file-alt Citations

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