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Enpp2 (NM_015744) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210991
Enpp2 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $790.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Enpp2 or NM_015744"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Enpp2
Synonyms AT; ATX; Auto; E-NPP 2; lysoPLD; N; Npps2; Pd; PD-; PD-Ialpha; Pdnp2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210991 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAGACAAGGCTGTTTCGGGTCATACCAGGTAATATCCTTGTTCACTTTTGCCATCGGCGTCAATC
TCTGCTTAGGATTCACAGCAAGTCGAATTAAGAGGGCCGAATGGGATGAAGGACCTCCCACAGTGTTATC
TGACTCTCCATGGACCAACACATCTGGATCCTGCAAAGGTAGATGCTTTGAGCTTCAAGAGGTTGGACCT
CCTGACTGTCGGTGTGACAACCTATGTAAGAGCTACAGCAGCTGCTGCCATGATTTTGATGAGCTCTGTT
TGAAAACAGCTCGAGGCTGGGAGTGCACCAAAGACAGATGTGGGGAAGTACGAAATGAGGAAAATGCCTG
TCACTGCTCAGAAGACTGCTTGTCCCGGGGAGACTGCTGTACCAACTATCAAGTGGTCTGCAAAGGAGAA
TCACACTGGGTAGATGATGACTGTGAAGAAATAAGAGTCCCTGAATGCCCTGCAGGGTTTGTCCGCCCTC
CGTTAATCATCTTCTCTGTGGATGGATTCCGTGCATCGTACATGAAGAAAGGCAGCAAGGTTATGCCCAA
CATTGAGAAACTGCGGTCCTGTGGCACCCATGCTCCCTACATGAGGCCTGTGTACCCTACAAAAACCTTC
CCTAATCTGTATACGCTGGCCACTGGTTTATATCCAGAATCCCATGGAATCGTTGGCAATTCAATGTATG
ACCCTGTCTTTGATGCTACTTTCCATCTTCGAGGGCGAGAGAAGTTTAACCATAGATGGTGGGGAGGCCA
ACCGCTATGGATTACAGCCACCAAGCAAGGGGTGAGAGCCGGGACATTCTTTTGGTCTGTGAGCATCCCT
CACGAGCGGAGAATCCTAACTATCCTTCAGTGGCTTTCCCTGCCAGACAATGAGAGGCCTTCAGTTTATG
CCTTCTACTCCGAGCAGCCTGATTTTTCTGGACACAAGTACGGCCCTTTTGGCCCTGAGATGACAAATCC
TCTGAGGGAGATTGACAAGACCGTGGGGCAGTTAATGGACGGACTGAAACAACTCAAGCTGCACCGTTGT
GTGAATGTTATCTTTGTTGGAGACCATGGAATGGAAGACGTGACATGTGACAGAACTGAGTTCTTGAGCA
ACTATCTGACTAACGTGGATGATATTACTTTAGTACCTGGAACTCTAGGAAGAATTCGACCCAAGATTCC
CAATAATCTTAAATATGACCCTAAAGCCATTATTGCTAACCTCACGTGTAAAAAACCAGATCAGCACTTT
AAGCCTTACATGAAACAGCACCTTCCCAAACGTTTGCACTATGCCAACAATCGGAGAATCGAGGATCTCC
ATTTATTGGTGGAACGCAGATGGCATGTTGCAAGGAAACCTTTGGACGTTTATAAGAAGCCGTCAGGAAA
ATGTTTTTTCCAGGGTGACCACGGCTTTGATAACAAGGTCAATAGCATGCAGACTGTTTTTGTAGGTTAT
GGCCCAACTTTTAAGTACAGGACTAAAGTGCCTCCATTTGAAAACATTGAACTTTATAATGTTATGTGCG
ATCTCCTAGGCTTGAAGCCAGCTCCCAATAATGGAACACATGGAAGTTTGAATCACCTGCTACGCACAAA
TACCTTTAGGCCAACCCTACCAGAGGAAGTCAGCAGACCCAATTACCCAGGGATTATGTACCTTCAGTCT
GATTTTGACCTGGGCTGCACCTGTGATGATAAGGTAGAGCCAAAGAACAAATTGGAAGAACTAAATAAAC
GCCTTCATACCAAAGGATCTACAGAAGAGAGACATCTCCTGTATGGACGACCTGCAGTGCTTTATCGGAC
TAGCTATGATATCTTATACCATACGGACTTTGAAAGTGGTTACAGTGAAATATTCTTAATGCCTCTCTGG
ACTTCTTATACCATTTCTAAGCAGGCTGAGGTCTCTAGCATCCCAGAGCACCTGACCAACTGTGTTCGCC
CTGATGTCCGTGTATCTCCTGGATTCAGTCAGAACTGTTTAGCCTATAAAAATGATAAACAGATGTCCTA
TGGATTCCTTTTTCCTCCCTATCTGAGCTCTTCCCCAGAAGCGAAATATGATGCATTCCTTGTAACCAAC
ATGGTTCCAATGTACCCTGCCTTCAAACGTGTTTGGACTTATTTCCAAAGGGTCTTGGTGAAGAAATATG
CGTCAGAAAGGAATGGGGTCAACGTAATAAGTGGACCGATCTTTGACTACAATTACGATGGCTTACGTGA
CATTGAGGATGAAATTAAACAGTATGTGGAAGGCAGCTCTATTCCTGTCCCTACCCACTACTACAGCATC
ATCACCAGCTGCCTGGACTTCACTCAGCCTGCAGACAAGTGTGATGGTCCTCTCTCTGTGTCTTCTTTCA
TCCTTCCTCACCGACCTGACAATGATGAGAGCTGTAATAGTTCCGAGGATGAGTCGAAGTGGGTAGAGGA
ACTCATGAAGATGCACACAGCTCGGGTGAGGGACATCGAGCATCTCACCGGTCTGGATTTCTACCGGAAG
ACTAGCCGTAGCTATTCGGAAATTCTGACCCTCAAGACATACCTGCATACATATGAGAGCGAGATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210991 protein sequence
Red=Cloning site Green=Tags(s)

MARQGCFGSYQVISLFTFAIGVNLCLGFTASRIKRAEWDEGPPTVLSDSPWTNTSGSCKGRCFELQEVGP
PDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGE
SHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTF
PNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIP
HERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRC
VNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHF
KPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGY
GPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQS
DFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLW
TSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTN
MVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYDGLRDIEDEIKQYVEGSSIPVPTHYYSI
ITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRK
TSRSYSEILTLKTYLHTYESEI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_015744
ORF Size 2586 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015744.1, NM_015744.2, NM_015744.3, NM_015744.4, NP_056559.1
RefSeq Size 3461 bp
RefSeq ORF 2589 bp
Locus ID 18606
UniProt ID Q9R1E6
Cytogenetics 15 D1
MW 98.9 kDa
Summary This gene encodes a member of the phosphodiesterase and nucleotide pyrophosphatase family of bifunctional enzymes that hydrolize phosphodiester bonds of various nucleotides. The encoded protein undergoes proteolytic processing to generate a mature protein with lysophospholipase D activity, catalyzing the cleavage of the choline group from lysophosphatidylcholine to produce lysophosphatidic acid. This gene is expressed in numerous tissues and participates in neural development, obesity, inflammation and oncogenesis. A complete lack of the encoded protein in mice results in aberrant vascular and neuronal development leading to embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate the mature protein. provided by RefSeq, Sep 2015
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Documents
Resources
"NM_015744" in other vectors (4)
SKU Description Size Price
MC204624 Enpp2 (untagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2, (10ug) 10 ug
$790.00
MG210991 Enpp2 (tGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2) 10 ug
$990.00
MR210991L3 Lenti ORF clone of Enpp2 (Myc-DDK-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2 10 ug
$1,090.00
MR210991L4 Lenti ORF clone of Enpp2 (mGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 2 (Enpp2), transcript variant 2 10 ug
$1,090.00

Plasmid Map for MR210991

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210991
Circular map for MR210991

file-alt Citations

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