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Ftsj3 (BC012281) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210907
Ftsj3 (Myc-DDK-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $767.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ftsj3
Synonyms AA537063; AU045295; C79843; D11Ertd400e; Epcs3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210907 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAGAAAGGCAAAGTAGGCAAGAGCCGGCGGGACAAGTTCTATCACTTGGCCAAGGAGACTGGTT
ATCGTTCGCGCTCTGCCTTCAAACTGATCCAGCTCAATCGCCGCTTTCAGTTCCTGCAGAAAGCCCGGGC
CTTGTTGGACCTGTGTGCTGCGCCTGGCGGATGGCTGCAGGTGGCTGCCAAGTTTATGCCTGTATCCAGT
CTTATTGTAGGAGTGGACCTCGTTCCAATCAAGCCTCTCCCCAATGTGGTGACTCTCCAGGAGGATATCA
CAACAGAACGCTGTAGGCAGGCCCTGAGGAAGGAGCTGAAGACCTGGAAAGTTGATGTTGTGCTTAATGA
TGGGGCTCCCAATGTGGGGGCTAGTTGGGTCCATGACGCTTACTCCCAAGCTCACCTAACTCTGATGGCT
CTGCGTTTGGCTTGTGATTTTTTGGCCCGTGGTGGTTGTTTCATCACAAAAGTCTTTCGTTCCCGAGACT
ATCAGCCCTTGCTGTGGATCTTCCAGCAGCTGTTCCACCGTGTCCAGGCCACGAAGCCCCAAGCCTCTCG
CCATGAATCTGCAGAAATCTTTGTAGTCTGTCAAGGATTCCTGGCTCCGGACAAGGTTGATGCTAAATTC
TTTGATCCCAAGTTTGCCTTTAAGGAGGTTGAAGTTCAGGCTAAAACTGTTACTGAGTTGGTGACAAGGA
AGAAGCCAAAGGCTGAAGGCTATGCCGAAGGTGACCTTACTCTTTATCACCGAACCTCAGTTACGGACTT
CTTGCGAGCTGCCAATCCTGTTGACTTCCTCTCCAAGGCCAGCGAAATCTCAATAGATGATGAAGAGTTG
GCACAGCACCCAGCTACAACTGAAGACATTAGAGTATGCTGTCAGGACATCAAAGTGCTGGGGCGAAAGG
AACTTAGGTCTCTTCTGAATTGGAGAACAAAGCTTCGGCGATATGTGGCTAAGAAGCTGAAAGAGCAAGC
AAAGGCATTGGACATCAGCCTCAGCTCAGAAGAAGAAGAAGAAGGTGATGAAGAAGAGGCAGTAGCTGAG
ACAAAGCAGGCGCCAGAGGAAGAAGAAGAGAGAGAGGAAGAACAGCTTAATCGGACTCTGGCAGAGATGA
AAGCCCAAGAAGTGGCAGAACTCAAGAGGAAGAAGAAGAAGTTGCTTCGTGAGCAGAGGAAGCAGCGGGA
GCGTGTCGAGCTGAAGATGGATTTGCCTGGGGTTTCTATTGCAGATGAGGGGGAGACAGGCATGTTCTCC
CTGCGAACCATCCGTGGCCAGCAGCTATTAGAGGAAGTAACACAAGGAGACATGAACGCTGCAGACACCT
TTCTGTCTGATCTGCCAAGGGATGACATCTATGTGTCAGATGCTGAGGACGATGATGATACTTCTCTGGA
AAGTGACCTGGATCCAGAAGAGCTGGCAGGAGTCAGGACACACTCGGATCTGAAGGAGCAGAAGTACTTG
CGGTTTACTAAAGTAGATGACAATAAAGAGGAAGGAGGAGAAAATCCATTGCTGGTACCACTGGAAGAAA
AGGCAGTACTGCAGGAAGAGCAAGCTAGCCTGTGGTTCTCCAAGGACGGCTTCAGTGGGATAGAGGATGA
TGCTGACGAAGCCCTGGAGATCAGTCAGGCACAGCTGCTGTACAAGAGCCGTCGGAAGGAGCAGCAGCCA
ACAGATCCACCTCCACCACCTACTAACTTGAAGACTGAGAAGAAATCTCCCCAGGGCCAGAATGAGGTCC
CTAAGGAGACAGAAGCCATCTTGGGGACAGAGGCTGTCACTGACCCTGGAGGGGAGGAAAGAGGCAACAG
CTCAGATAGTGACAGCAGCAGTAGTGAGGATGAAGATAGCTGGAAAGTGTCCCGTGGTGTGAAGCGAGGC
CGTGGCTCTAAGGCAGATGAGGATGGGTTTGAGGTGGTGCCTATTCAGGACCCAGTGAAATACCGAATTC
TGGACCCTGAAGGCCTTGCTCTAGGTGCTGTTATTGCCTCTTCAAAAAAGGCAAAGAGAGACCTCATAGA
TAACTCGTTCAACCGGTATGCTTTTAATGAAGAGGAGGGAGAACTTCCTGAGTGGTTTGCACAGGAAGAA
AAGCAGCATCGGATACGCCAGCTGCCTGTTGATAAGAAGGAGGTGGAACATTACCGAAAACGCTGGCGGG
AAATCAATGCACGGCCCATCAAGAAAGTAGCTGAGGCCAAGGCCAGAAAGAAACGGAGGATGCTGAAGAA
GCTGGAACAGACCAAAAAGAAGGCAGAAGCTGTGGTGAATACAGTGGACATCTCAGAACGGGAAAAGGTG
GCACAGCTGGCCAGTCCAGTACATACTGCTGGACTTGGCATAGCAGTCATTGTTAGAGTTTTTCATCTCG
CCTTTAAGGGTGTGAACCACAATAATCTCCCAGGATGCAAGCCAAAAGGTCACGCAAAGATAGTTGTTGC
TCGAATGAAGAAGGATCAGAGAGCACAACAACGGAAGGAACAGAAGAAAAAACACAAGCGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210907 protein sequence
Red=Cloning site Green=Tags(s)

MGKKGKVGKSRRDKFYHLAKETGYRSRSAFKLIQLNRRFQFLQKARALLDLCAAPGGWLQVAAKFMPVSS
LIVGVDLVPIKPLPNVVTLQEDITTERCRQALRKELKTWKVDVVLNDGAPNVGASWVHDAYSQAHLTLMA
LRLACDFLARGGCFITKVFRSRDYQPLLWIFQQLFHRVQATKPQASRHESAEIFVVCQGFLAPDKVDAKF
FDPKFAFKEVEVQAKTVTELVTRKKPKAEGYAEGDLTLYHRTSVTDFLRAANPVDFLSKASEISIDDEEL
AQHPATTEDIRVCCQDIKVLGRKELRSLLNWRTKLRRYVAKKLKEQAKALDISLSSEEEEEGDEEEAVAE
TKQAPEEEEEREEEQLNRTLAEMKAQEVAELKRKKKKLLREQRKQRERVELKMDLPGVSIADEGETGMFS
LRTIRGQQLLEEVTQGDMNAADTFLSDLPRDDIYVSDAEDDDDTSLESDLDPEELAGVRTHSDLKEQKYL
RFTKVDDNKEEGGENPLLVPLEEKAVLQEEQASLWFSKDGFSGIEDDADEALEISQAQLLYKSRRKEQQP
TDPPPPPTNLKTEKKSPQGQNEVPKETEAILGTEAVTDPGGEERGNSSDSDSSSSEDEDSWKVSRGVKRG
RGSKADEDGFEVVPIQDPVKYRILDPEGLALGAVIASSKKAKRDLIDNSFNRYAFNEEEGELPEWFAQEE
KQHRIRQLPVDKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKKLEQTKKKAEAVVNTVDISEREKV
AQLASPVHTAGLGIAVIVRVFHLAFKGVNHNNLPGCKPKGHAKIVVARMKKDQRAQQRKEQKKKHKRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC012281
ORF Size 2514 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC012281, AAH12281
RefSeq Size 2883 bp
RefSeq ORF 2516 bp
Locus ID 56095
Cytogenetics 11 68.89 cM
MW 95.2 kDa
Summary RNA 2'-O-methyltransferase involved in the processing of the 34S pre-rRNA to 18S rRNA and in 40S ribosomal subunit formation.UniProtKB/Swiss-Prot Function
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"BC012281" in other vectors (4)
SKU Description Size Price
MC201000 Ftsj3 (untagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961), (10ug) 10 ug
$768.00
MG210907 Ftsj3 (tGFP-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961) 10 ug
$967.00
MR210907L3 Lenti ORF clone of Ftsj3 (Myc-DDK-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961) 10 ug
$1,067.00
MR210907L4 Lenti ORF clone of Ftsj3 (mGFP-tagged) - Mouse FtsJ homolog 3 (E. coli) (cDNA clone MGC:18657 IMAGE:4012961) 10 ug
$1,067.00

Plasmid Map for MR210907

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210907
Circular map for MR210907

file-alt Citations

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