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Gpld1 (NM_008156) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210904
Gpld1 (Myc-DDK-tagged) - Mouse glycosylphosphatidylinositol specific phospholipase D1 (Gpld1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $771.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpld1
Synonyms 6330541J12Rik; AW546131
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210904 representing NM_008156
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGCAGGCAGGCTGTGGTCTAGCCTGCTGCTTCTGCTGCCTCTTTTCTGCTCTAAAAGCTCATCTT
GTGGTCTCTCAACACATGTAGAAATAGGACACAGGGCTCTGGAGTTTCTTCGGCTTCAAGATGGACGCAT
TAACTACAAAGAGCTGATCTTAGAGCACCAGGACGCATATCAGGCTGGGACCGTGTTTCCTGATGCCTTT
TATCCTAGCATCTGCAAAAGAGGAAAATATCATGACGTTTCTGAGAGGACTCACTGGACTCCATTTCTTA
ACGCCAGCATCCATTATATTCGAGAGAACTACCCTCTGCCCTGGGAGAAGGACACAGAGAAGTTGGTGGC
TTTCTTGTTTGGAATCACCTCCCACATGGTCGCTGACGTGAGCTGGCATAGCCTGGGTATTGAACAAGGG
TTCCTCAGGACAATGGGAGCTATCGATTTTTACAACTCTTACTCTGACGCTCACTCGGCTGGTGATTTTG
GAGGAGATGTGTTGAGCCAGTTTGAATTTAATTTTAATTACCTCTCACGGCGCTGGTACGTGCCCGTCAG
GGATCTTCTGAGAATTTATGATAATCTCTATGGTCGGAAAGTCATCACCAAAGACGTCCTTGTTGATTGC
ACCTACCTTCAGTTCCTGGAAATGCACGGGGAGATGTTTGCTGTTTCCAAGCTCTATTCCACGTACTCTA
CAAAGTCCCCATTTCTGGTGGAGCAGTTCCAAGACTATTTCCTCGGAGGTCTGGATGACATGGCATTCTG
GTCCACGAACATTTACCGTTTGACCAGCTTTATGCTGGAGAACGGGACCAGTGACTGCAACCTGCCTGAG
AACCCCCTGTTCATCTCCTGTGATGGCAGGAACCACACCCTCAGTGGCTCAAAAGTGCAGAAAAATGATT
TTCACAGGAATTTGACCATGTTCATAAGTAGAGACATCAGGAAAAACCTCAATTACACAGAAAGAGGCGT
GTTCTACAGCACAGGCTCCTGGGCCCCGGAATCTGTCACCTTTATGTACCAGACTCTGGAGAGGAACCTG
AGGCTGATGCTTGCTGGCAGCTCTCAGAAAAATCTGAATCATGTCTCCAGCCCCTCTGCCTCCTACACCC
TGTCTGTCCCCTATGCCAGGCTCGGCTGGGTCATGACCTCAGCTGACCTCAACCAGGATGGGCACGGAGA
CCTCGTGGTGGGTGCACCAGGCTACAGCCACCCTGGCCGCTTCCAGATTGGGCGGGTGTACATCATCTAT
GGCAACGATCTGGGCCTGCCTCCCATCGACCTGGACTTAGACAAGGAGGCCCATGGGATCCTGGAGGGCT
TCCAGCCTTCAGGTCGCTTTGGCTCAGCTTTGGCCGTGCTGGACTTCAACCAGGATGGGCTGCCTGACCT
GGCTGTGGGCGCTCCCTCCGTGGGCTCAGGGCAGCTCACCTACAATGGCTCGGTGTATGTCTACTATGGT
TCCCAGCAAGGGAGGCTGTCTTCTTCCCCTAACGTCACCATCTCCTGCAAGGACACCTACTGTAACTTGG
GCTGGACCCTCTTGGCAACAGATGCAGACGGAGATGGCCGGCACGATCTGGTCATCAGCTCCCCTTTCGC
ACCAGGTGGAGGGAAGCAGAGAGGAATTGTGGCCACCTTTTATTCTCACCCCAGACGGAATGACAAAGAA
TTACTGACTTTGGAGGAAGCTGACTGGAAGGTGAATGGGGAGGAGGACTTCTCGTGGTTTGGATACTCCC
TTCACGGGGTCACAGTGGCAAACAGAAGCCTGCTGCTGATTGGGAGCCCGACCTGGAAGAATGTCAGCAG
GATGGCACGCTCAAGCCACAAAAAGAACCAAGAGGAAAAGAGCCTGGGAAAGGTGTACGGCTACTTTCTC
CCGAACCGCCAGAGCACAATCACTATCTCGGGAGATAAGGCGATGGGAAAGCTGGGTACCTCCCTGTCCA
GTGGTTATGTGAGAGTGAATGGGACCCTAACCCAAGTCCTGCTGGTTGGAGCCCCTACTCATGATGACGT
GTCTAAAATGGCATTCCTAACCATGACCCTGCACCAGGGTGGAGCCACTCGGATGTACGAACTAGCTCCC
GAGAAGACACAGCCTGCTCTGCTCAGCACATTCAGTGGAGACCGACGCTTTTCTCGATTTGGTAGTGTTC
TGCACCTGACTGACCTGGATGATGATGGCTTAGATGAAATCATCATGGCTGCCCCACTGAGGATAACAGA
TGTCACTTCCGGACTGCTAGGGGGAGAAGATGGGCGAGTGTACATTTATAATGGCATGTATACCACCTTA
GGTGACATGACCGGCAAATGCAAGTCCTGGATGACTCCATGTCCGGAGGAGAAGGCACAGTATGTACTAA
CTTCTCCAGAGGCAAGTTCAAGGTTTGGGAGCTCGCTGGTGTCCGTGCGGTCTAAGGGAAGGAACCAAGT
GGTTGTTGCTGCTGGAAGGAGTTCTTGGGGAGCCCGGCTCTCTGGGGCACTTCATGTCTACAGCTTCAGC
TCAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210904 representing NM_008156
Red=Cloning site Green=Tags(s)

MSAGRLWSSLLLLLPLFCSKSSSCGLSTHVEIGHRALEFLRLQDGRINYKELILEHQDAYQAGTVFPDAF
YPSICKRGKYHDVSERTHWTPFLNASIHYIRENYPLPWEKDTEKLVAFLFGITSHMVADVSWHSLGIEQG
FLRTMGAIDFYNSYSDAHSAGDFGGDVLSQFEFNFNYLSRRWYVPVRDLLRIYDNLYGRKVITKDVLVDC
TYLQFLEMHGEMFAVSKLYSTYSTKSPFLVEQFQDYFLGGLDDMAFWSTNIYRLTSFMLENGTSDCNLPE
NPLFISCDGRNHTLSGSKVQKNDFHRNLTMFISRDIRKNLNYTERGVFYSTGSWAPESVTFMYQTLERNL
RLMLAGSSQKNLNHVSSPSASYTLSVPYARLGWVMTSADLNQDGHGDLVVGAPGYSHPGRFQIGRVYIIY
GNDLGLPPIDLDLDKEAHGILEGFQPSGRFGSALAVLDFNQDGLPDLAVGAPSVGSGQLTYNGSVYVYYG
SQQGRLSSSPNVTISCKDTYCNLGWTLLATDADGDGRHDLVISSPFAPGGGKQRGIVATFYSHPRRNDKE
LLTLEEADWKVNGEEDFSWFGYSLHGVTVANRSLLLIGSPTWKNVSRMARSSHKKNQEEKSLGKVYGYFL
PNRQSTITISGDKAMGKLGTSLSSGYVRVNGTLTQVLLVGAPTHDDVSKMAFLTMTLHQGGATRMYELAP
EKTQPALLSTFSGDRRFSRFGSVLHLTDLDDDGLDEIIMAAPLRITDVTSGLLGGEDGRVYIYNGMYTTL
GDMTGKCKSWMTPCPEEKAQYVLTSPEASSRFGSSLVSVRSKGRNQVVVAAGRSSWGARLSGALHVYSFS
SD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008156
ORF Size 2526 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008156.2, NP_032182.2
RefSeq Size 4659 bp
RefSeq ORF 2529 bp
Locus ID 14756
Cytogenetics 13 10.79 cM
MW 94.1 kDa
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Documents
Resources
"NM_008156" in other vectors (4)
SKU Description Size Price
MC205506 Gpld1 (untagged) - Mouse glycosylphosphatidylinositol specific phospholipase D1 (Gpld1), (10ug) 10 ug
$772.00
MG210904 Gpld1 (tGFP-tagged) - Mouse glycosylphosphatidylinositol specific phospholipase D1 (Gpld1) 10 ug
$971.00
MR210904L3 Lenti ORF clone of Gpld1 (Myc-DDK-tagged) - Mouse glycosylphosphatidylinositol specific phospholipase D1 (Gpld1) 10 ug
$1,071.00
MR210904L4 Lenti ORF clone of Gpld1 (mGFP-tagged) - Mouse glycosylphosphatidylinositol specific phospholipase D1 (Gpld1) 10 ug
$1,071.00

Plasmid Map for MR210904

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210904
Circular map for MR210904

file-alt Citations

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