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Bicd1 (NM_009753) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210891
Bicd1 (Myc-DDK-tagged) - Mouse bicaudal D homolog 1 (Drosophila) (Bicd1), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $765.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bicd1
Synonyms B830009D06Rik; bic-D 1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210891 representing NM_009753
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCAGAGGAGGCGCTGAAGACGGTAGACCAATATAAGACTGAAATAGAGAGACTGACCAAGGAGC
TCACAGAAACGACCCATGAGAAGATCCAGGCTGCAGAGTATGGGCTGGTGGTGCTGGAGGAGAAACTGAC
TCTCAAGCAACAGTATGACGAGCTGGAGGCCGAGTATGACGGCCTCAAACAAGAGCTGGAGCAGCTCAGA
GAGGCATTTGGGCAGTCCTTCTCTATCCACCGTAAAGTAGCTGAGGATGGAGAGACTCGGGAGGAAACAC
TTCTTCAGGAGTCTGCATCCAAAGAAGCTTACTATCTAAACAAGATCTTGGAGATGCAGAATGAGCTGAA
GCAGAGCCGGGCTGTGGTTACAAATGTCCAGGCAGAAAATGAGAGGCTCTCGGCCGTTGTACAGGAACTG
AAAGAGAACAACGAGATGGTGGAGCTACAGAGAATACGAATGAAAGATGAAATCCGAGAATATAAATTCC
GGGAGGCCCGACTCCTTCAGGACTACACAGAATTGGAAGAAGAAAATATCACATTGCAGAAACTAGTGTC
CACATTGAAGCAGAACCAGGTTGAATACGAAGGCTTAAAGCATGAAATTAAGCGATTTGAAGAAGAAACA
GTATTACTGAATAGCCAACTAGAAGATGCCATTCGACTGAAAGAGATTGCAGAGCATCAGTTGGAAGAAG
CCCTTGAGACACTGAAGAATGAAAGAGAGCAAAAGAACAACCTGAGGAAGGAGCTGTCCCAGTACATCAA
CCTCAGTGATAGCCACATCAGCATCTCAGTAGATGGGCTCAAGTTTGCTGAGGATGGAAGTGAGCCAAAT
AACGATGACAAGATGAATGGACATATCCATGGGCCTCTTGGGAAACTGAATGGAGACTATCGTACTCCCA
CTACCAGGAAAGGAGAGTCCCTACACCCTGTGTCTGACTTATTCAGTGAGTTGAACATTTCAGAAATACA
GAAATTGAAGCAGCAGCTTATTCAGGTAGAGAGGGAAAAAGCTATTCTTCTGGCCAACCTCCAGGAGTCA
CAAACCCAGCTAGAACATACCAAGGGGGCACTGACAGAGCAGCATGAGCGAGTGCACCGGCTCACAGAGC
ATGTCAATGCTATGAGGGGCTTGCAAAACAGCAAAGAGATCAAGGCTGAGCTAGATTGTGAGAAGGGCCG
GAACTCAGCGGAGGAAGCTCATGACTATGAGGTGGACATCAATGGCTTAGAGATCCTTGAGTGCAAATAT
AGAGTGGCCGTCACAGAAGTCATTGATTTGAAAGCAGAAATTAAGGCCTTAAAGGAAAAATATAATAAAT
CTGTAGAAAATTATACAGAAGAAAAGACCAAATATGAGAGTAAGATCCAAATGTATGATGAACAAGTGAC
AAACCTTGAGAAGACATCTAAGGAGAGTGGTGAGAAGATGGCCCACATGGAGAAGGAGTTGCAAAAGATG
ACTGGCATAGCCAACGAAAATCACAACACCCTCAATACAGCCCAGGATGAGTTGGTGACATTTAGTGAGG
AACTAGCCCAGCTTTACCACCATGTGTGTCTATGTAATAATGAAACTCCCAATAGAGTCATGTTGGACTA
CTATAGACAAAGCAGAGTTACTCGAAGTGGCAGCCTCAAGGGGCCTGATGATCCCAGAGGGCTTTTGTCG
CCAAGATTATCCAGGAGGGGTGTGTCATCCCCTGTAGAATCAAGGACATCATCTGAACCAGTTTCAAAGG
AAAATACAGAGACTAGTAAAGAGCCAAGTCCAACTAAGACTCCCACAATCTCTCCTGTTATTACTGCCCC
ACCATCATCTCCAGTTTTGGATACCAGTGATATTCGCAAAGAGCCAATGAATATCTACAACCTCAATGCC
ATAATCCGGGACCAAATCAAGCATCTGCAGAAGGCAGTGGACAGGTCCTTACAGCTGTCTCGTCAAAGAG
CAGCAGCCCGAGAGTTGGCCCCCATGATTGATAAGGACAAGGAAGCCTTAATGGAAGAGATTCTCAAACT
GAAGTCCTTGCTGAGCACCAAACGGGAGCAGATTGCGACACTGAGGGCAGTGTTGAAAGCCAATAAGCAG
ACAGCTGAAGTGGCTTTAGCTAACCTGAAGAACAAATATGAAAATGAAAAGGCAATGGTTACTGAAACAA
TGACAAAACTGAGGAATGAACTAAAGGCTTTGAAGGAAGATGCTGCAACCTTCTCATCCCTGAGAGCAAT
GTTTGCAACAAGATGCGATGAATATGTCACACAGTTGGATGAGATGCAGAGACAGTTAGCAGCTGCAGAA
GATGAGAAGAAGACTCTAAACACTTTATTGCGAATGGCTATCCAGCAAAAACTCGCCCTGACACAGAGGC
TGGAGGACTTAGAGTTTGACCATGAGCAGTCCCGACGCAGCAAAGGCAAACTTGGAAAGAGCAAGATCGG
CAGCCCTAAAATTGTCAGCAGCCTGCTGCCTCCGTACCGCCACAGTGCTCACAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210891 representing NM_009753
Red=Cloning site Green=Tags(s)

MAAEEALKTVDQYKTEIERLTKELTETTHEKIQAAEYGLVVLEEKLTLKQQYDELEAEYDGLKQELEQLR
EAFGQSFSIHRKVAEDGETREETLLQESASKEAYYLNKILEMQNELKQSRAVVTNVQAENERLSAVVQEL
KENNEMVELQRIRMKDEIREYKFREARLLQDYTELEEENITLQKLVSTLKQNQVEYEGLKHEIKRFEEET
VLLNSQLEDAIRLKEIAEHQLEEALETLKNEREQKNNLRKELSQYINLSDSHISISVDGLKFAEDGSEPN
NDDKMNGHIHGPLGKLNGDYRTPTTRKGESLHPVSDLFSELNISEIQKLKQQLIQVEREKAILLANLQES
QTQLEHTKGALTEQHERVHRLTEHVNAMRGLQNSKEIKAELDCEKGRNSAEEAHDYEVDINGLEILECKY
RVAVTEVIDLKAEIKALKEKYNKSVENYTEEKTKYESKIQMYDEQVTNLEKTSKESGEKMAHMEKELQKM
TGIANENHNTLNTAQDELVTFSEELAQLYHHVCLCNNETPNRVMLDYYRQSRVTRSGSLKGPDDPRGLLS
PRLSRRGVSSPVESRTSSEPVSKENTETSKEPSPTKTPTISPVITAPPSSPVLDTSDIRKEPMNIYNLNA
IIRDQIKHLQKAVDRSLQLSRQRAAARELAPMIDKDKEALMEEILKLKSLLSTKREQIATLRAVLKANKQ
TAEVALANLKNKYENEKAMVTETMTKLRNELKALKEDAATFSSLRAMFATRCDEYVTQLDEMQRQLAAAE
DEKKTLNTLLRMAIQQKLALTQRLEDLEFDHEQSRRSKGKLGKSKIGSPKIVSSLLPPYRHSAHN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009753
ORF Size 2505 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009753.4, NP_033883.2
RefSeq Size 9667 bp
RefSeq ORF 2508 bp
Locus ID 12121
UniProt ID Q8BR07
Cytogenetics 6 G3
MW 95.9 kDa
Summary Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_009753" in other vectors (4)
SKU Description Size Price
MC203937 Bicd1 (untagged) - Mouse bicaudal D homolog 1 (Drosophila) (Bicd1), transcript variant 2, (10ug) 10 ug
$765.00
MG210891 Bicd1 (tGFP-tagged) - Mouse bicaudal D homolog 1 (Drosophila) (Bicd1) 10 ug
$965.00
MR210891L3 Lenti ORF clone of Bicd1 (Myc-DDK-tagged) - Mouse bicaudal D homolog 1 (Drosophila) (Bicd1), transcript variant 2 10 ug
$1,065.00
MR210891L4 Lenti ORF clone of Bicd1 (mGFP-tagged) - Mouse bicaudal D homolog 1 (Drosophila) (Bicd1), transcript variant 2 10 ug
$1,065.00

Plasmid Map for MR210891

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210891
Circular map for MR210891

file-alt Citations

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