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Sidt2 (NM_172257) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210871
Sidt2 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $762.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sidt2
Synonyms B930096O19; BC023957; CGI-40
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210871 representing NM_172257
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCGCCTGGCGTCTGCCCTTGTGCGTGCTCTTGGTGGCCTCCGTCGAGAGCCACCTGGGGGCCCTGG
GGCCCAAGAACGTCTCGCAGAAAGACGCGGAGTTTGAGCGCACCTACGCGGACGACGTCAACAGCGAGCT
GGTCAACATCTACACCTTCAACCACACCGTGACCCGCAACCGGACCGAGGGTGTGCGAGTGTCTGTGAAT
GTCCTGAACAAGCAGAAAGGGGCGCCTTTGCTGTTTGTGGTCCGCCAGAAGGAGGCTGTTGTGTCCTTCC
AGGTGCCCCTAATCCTTCGAGGACTATATCAGCGGAAGTACCTCTACCAAAAAGTGGAACGAACTCTGTG
TCAGCCCCCCACCAAGAATGAGTCTGAGATCCAGTTTTTCTATGTGGACGTGTCTACCCTGTCACCCGTC
AATACCACTTACCAGCTCCGAGTCAACCGTGTGGACAATTTTGTGCTCAGGACTGGAGAGCTGTTTACTT
TTAATACCACTGCAGCCCAGCCCCAGTACTTCAAATACGAGTTTCCTGATGGTGTGGACTCGGTAATTGT
CAAGGTGACCTCCAAGAAGGCCTTCCCCTGCTCAGTCATCTCCATCCAAGATGTCCTGTGCCCTGTCTAT
GATCTGGACAACAATGTAGCCTTCATTGGCATGTACCAGACGATGACTAAGAAGGCAGCCATCACTGTGC
AGCGGAAAGACTTCCCCAGCAACAGCTTCTATGTGGTGGTGGTAGTGAAGACTGAGGACCAGGCCTGCGG
AGGGTCCTTGCCCTTCTACCCTTTTGTGGAAGATGAGCCAGTGGATCAAGGGCACCGTCAGAAAACACTG
TCAGTGCTGGTCTCTCAGGCTGTCACATCTGAGGCCTATGTTGGTGGGATGCTCTTTTGCCTGGGCATAT
TCTTGTCCTTCTACCTGCTGACTGTGCTGCTGGCCTGTTGGGAGAACTGGAGGCAAAGGAAGAAGACCTT
GCTGTTGGCCATAGACCGAGCCTGCCCAGAAAGTGGTCACGCCCGGGTCTTGGCTGATTCATTTCCTGGC
AGTGCCCCTTACGAGGGTTACAACTATGGCTCCTTTGAAAATGGTTCCGGATCCACTGACGGGTTGGTTG
AAAGCGCAGGTTCAGGGGACCTCTCCTACAGTTACCAGGACCGCTCCTTTGACGCAGTGGGTCCTCGGCC
TCGACTGGACTCCATGAGCTCCGTGGAAGAGGATGACTACGACACACTGACTGACATCGACTCAGACAAA
AACGTCATTCGAACCAAGCAATACCTCTGTGTGGCTGATCTGGCACGAAAGGACAAACGTGTTTTGCGGA
AAAAGTACCAGATTTACTTCTGGAACATAGCCACCATTGCGGTCTTCTACGCACTTCCTGTGGTGCAGCT
GGTGATCACCTACCAGACGGTGGTGAATGTCACAGGGAACCAGGACATCTGCTACTACAACTTCCTCTGT
GCCCACCCGCTGGGCAACCTCAGCGCCTTCAACAACATCCTCAGCAACTTGGGGTACATCCTGCTGGGGC
TGCTCTTCCTGCTCATCATCCTGCAGCGAGAGATCAATCATAACCGGGCCCTGCTGCGGAATGACCTCTA
TGCTCTGGAGTGTGGGATCCCCAAACACTTTGGTCTGTTTTACGCCATGGGCACAGCACTGATGATGGAG
GGGCTACTTAGTGCCTGTTACCACGTCTGCCCCAACTACACCAACTTCCAGTTTGATACCTCCTTCATGT
ACATGATTGCTGGCCTCTGCATGCTGAAGCTCTACCAGAAGCGGCACCCAGATATCAACGCCAGTGCCTA
CAGTGCATATGCCTGCTTGGCCATCGTCATCTTCTTCTCCGTTCTGGGCGTGGTGTTTGGCAAAGGGAAC
ACGGCCTTCTGGATTGTCTTCTCCGTCATTCACATCATCTCCACCCTGCTCCTCAGCACTCAGCTCTATT
ACATGGGCCGCTGGAAGCTGGACTCCGGGATCTTCCGCCGCATCCTCCATGTGCTCTACACAGACTGCAT
CCGGCAGTGCAGCGGGCCCCTTTACACGGACCGCATGGTGCTTCTGGTCATGGGCAACATTATCAACTGG
TCGCTGGCTGCATACGGACTCATCATGCGCCCCAATGACTTTGCTTCCTACTTGCTGGCAATTGGCATCT
GCAACCTGCTGCTTTATTTCGCCTTCTACATCATCATGAAGCTCCGGAGCGGCGAGAGGATCAAGCTCAT
CCCTCTGCTCTGCATCGTCTGCACCTCCGTGGTCTGGGGCTTCGCGCTCTTCTTCTTCTTCCAGGGACTG
AGCACGTGGCAGAAAACCCCCGCAGAGTCCAGGGAGCACAACCGCGACTGCATCCTCCTCGACTTCTTTG
ATGACCACGATATCTGGCACTTCCTGTCCTCCATTGCCATGTTTGGGTCCTTCCTGGTTTTGCTGACGTT
GGATGACGACTTGGACACAGTACAGCGGGACAAGATCTATGTCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210871 representing NM_172257
Red=Cloning site Green=Tags(s)

MIAWRLPLCVLLVASVESHLGALGPKNVSQKDAEFERTYADDVNSELVNIYTFNHTVTRNRTEGVRVSVN
VLNKQKGAPLLFVVRQKEAVVSFQVPLILRGLYQRKYLYQKVERTLCQPPTKNESEIQFFYVDVSTLSPV
NTTYQLRVNRVDNFVLRTGELFTFNTTAAQPQYFKYEFPDGVDSVIVKVTSKKAFPCSVISIQDVLCPVY
DLDNNVAFIGMYQTMTKKAAITVQRKDFPSNSFYVVVVVKTEDQACGGSLPFYPFVEDEPVDQGHRQKTL
SVLVSQAVTSEAYVGGMLFCLGIFLSFYLLTVLLACWENWRQRKKTLLLAIDRACPESGHARVLADSFPG
SAPYEGYNYGSFENGSGSTDGLVESAGSGDLSYSYQDRSFDAVGPRPRLDSMSSVEEDDYDTLTDIDSDK
NVIRTKQYLCVADLARKDKRVLRKKYQIYFWNIATIAVFYALPVVQLVITYQTVVNVTGNQDICYYNFLC
AHPLGNLSAFNNILSNLGYILLGLLFLLIILQREINHNRALLRNDLYALECGIPKHFGLFYAMGTALMME
GLLSACYHVCPNYTNFQFDTSFMYMIAGLCMLKLYQKRHPDINASAYSAYACLAIVIFFSVLGVVFGKGN
TAFWIVFSVIHIISTLLLSTQLYYMGRWKLDSGIFRRILHVLYTDCIRQCSGPLYTDRMVLLVMGNIINW
SLAAYGLIMRPNDFASYLLAIGICNLLLYFAFYIIMKLRSGERIKLIPLLCIVCTSVVWGFALFFFFQGL
STWQKTPAESREHNRDCILLDFFDDHDIWHFLSSIAMFGSFLVLLTLDDDLDTVQRDKIYVF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172257
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172257.4, NP_758461.1
RefSeq Size 4221 bp
RefSeq ORF 2499 bp
Locus ID 214597
UniProt ID Q8CIF6
Cytogenetics 9 A5.2
MW 95 kDa
Summary Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA and DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365, PubMed:28724756). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA (PubMed:27846365). Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (PubMed:26067272).UniProtKB/Swiss-Prot Function
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"NM_172257" in other vectors (4)
SKU Description Size Price
MC221923 Sidt2 (untagged) - Mouse SID1 transmembrane family, member 2 (Sidt2), (10ug) 10 ug
$839.00
MG210871 Sidt2 (tGFP-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2) 10 ug
$789.00 $962.00
MR210871L3 Lenti ORF clone of Sidt2 (Myc-DDK-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2) 10 ug
$1,062.00
MR210871L4 Lenti ORF clone of Sidt2 (mGFP-tagged) - Mouse SID1 transmembrane family, member 2 (Sidt2) 10 ug
$1,062.00

Plasmid Map for MR210871

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210871
Circular map for MR210871

file-alt Citations

Systemic RNA Interference Deficiency-1 (SID-1) extracellular domain selectively binds long double-stranded RNA and is required for RNA transport by SID-1
Li, Koutmou, Leahy et al
J. Biol. Chem. (2015)
AI Summary: OriGene contributed cDNA clones for SID-1, mouse SIDT1, and mouse SIDT2, which were used in this study to express and analyze the RNA-binding properties of these proteins.
Snippet: EXPERIMENTAL PROCEDURES Molecular cloning of SID-1 homologs- cDNAs for SID-1, mouse SIDT1 and mouse SIDT2 were purchased from OriGene. A synthetic SID-1 gene with optimized codon frequency for mammalian expression was ordered from GenScript ... Cite SaveSaving
Showing 1-1 of 1 papers.
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