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Fastkd1 (NM_177244) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210852
Fastkd1 (Myc-DDK-tagged) - Mouse FAST kinase domains 1 (Fastkd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $760.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fastkd1
Synonyms 5330408N05Rik; mKIAA1800
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210852 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCGGCTACGAAGCATCTCTTTACTCTCTTGGAGAGCATTTCCGCTCCGCCCCTTCAGTTGTGAGT
CATTAATTACTCAGATGCAGAAGTGTACAAACGAAGAACAAGTGTTTGACCTTATTGAGACGAATACGGC
AACGCTCTCCGAACAGCAAGTGGGCTGTGCGTTTAATGTACTTTGGCAGTTTCAGAAGCAGAAGACCGTC
TTGGAAAAAAACGTTGACCATGTTAGAAACCATCCTCAGTTTCTTACTCTTTGCAGCATTACAACAAATC
ACATCCCAGCAATGAGTGATGCTACCCTGGTGGATGTGTTATACAGCATAAAGCAGTTTGCAGTTGAATC
CCATCACCCACTAATCGAAGCACTGGTTACAGAAGCATGGAAAAGACTGGAAAGGTTCGATACTAATGTG
CTGTCCATATTTTCCACTTGCTTGGCCGATCAGCATCTGTATTTCAGTCCATTAATGGGAAAAATAGCTG
ATATTGTAAATAGGAGACTGGAAACCATCCAGGACTTAAGGGCCTTATCTGTCTTAATGGTCAGCATTTC
TTCATTAATATCACCGTGTTTCCAGGAACGACTGGTGATCAGAACAGAACTTCTTTTTGACACTGTAAAT
TCTTCCAAAGTCAACATTGCAAGAAGGATATTACTGTTTCTTCGAAATGTCAAATACAGTCATTACCCAC
TGTTAGAGAGGTGTAATCAAGTGTTTATAAGGAACATGAGCCATCTTGATCTGGAGTCCATCAGTAAAAT
CCTTAACCTGTATCAGTTTCTACAGTTCCACAGCTTTGAATTTGTTGAAGCAGCTCGAGGGAGGCTGGCT
GAGATGACCCTCCCTTCTGATCATCCTGAAAGCTTTGTCCGGTTGTTTGCCGCTCTGGGGCCGGTGGCCA
GACCTGAGATAAAGAAACAGCTTAAATCAACTATACTACTGCTGTCAGAGGAGCTGAGCAGCCAGCAAGC
GCTGATCGTGTTGGGAGCAATGGAAGATATGGAAAGCAGAAATTCACACTTGCTTAAAAAGATTGTTTCT
GTTCTGTACAAACATTTGGATAACTATAAATCGATAGAGCTATTGAAGATAATACAAGCATTGACTTTTC
TACATTTTCAAAGTAAGGAGCTTTTTATGAAACTCAGAGAATTACTTCTCAGTCGTTTGGAAGCTAGTGT
CATACCCAGTGAGATTTCTGTTCTGGTCTCTGCTCTGTCCATGCTTCCACATCCGCACCTCAGTGAAACA
GCGGTCTCCCGGATTGAAGCAGTTCTACCACAGTGTGACTTCAGGGAACTGAATGACTTGGTAGTATATC
TCATGCGCTGGATTCAGAGTGATCTCGTCTGCCTGGCTAGTACTACTGGAAAGCAACTGGACCTCCTTCA
AAAACTAGATCAACTGGGCCGTCACCGGCTGCAACAGAGCACCAACTTGGATCTTCTGTGGGAGGAGCTC
AAGTCTTTGAAGGGAGAATGGCTTCACGAATCCTTAGTTGAAGAATCCATCGCTGCTTTACTGCGTTTCA
TGGATGAAATAGATTACAGCAATATTGCCAAGGTCGCGTCTTTTCTTTCCAGAACTAACTACCTCAACAC
TCTGCTGCTGGATAGGATCGCTTCAGTGGCCATTCAGCAGGTTGAAAAGATCCATCCCTTTTCAGTCCTT
GCTATTATTCTCCCATTCAGCATTCTGAACTATGATCCACCTCAAAAGGATGAATTCTTTGGAGCGTGTG
TCCAATGCTGTAATTCTTACTTAGGTACATTGGATCCTGGTACATTAGTGTTTCTTGGTTTTTCTTTGGC
CGTACTTGAATATTTTCCAGAAGATCTGCTAAAGAAAATGTTTAACATCGAGTTCCTTGCCAGATTAGAT
TCCCAGCTTGAAATTTTACCTTCAAGTCTGAGTGCAAGGATCCAGTTCCGCCTCATGGAGTTAAATAGAG
CAGTCTGCTTGGAGTGCCCCGAGCTGCAGGTTCCATGGTTTCATGACCGCTTTTGTCAGCGACAGTTTAA
CAAAGACACTGGTGTTATGAACGGAGCCCAACAACAGATCTATAAAATGTTAGCAGAGGTCCTGGGAGGA
CACCAATGTGTGAAGCCTTCCGCTCTTTCACCGTATTACCACACAGTGGGTTTTGAATGCATTTTGGATA
AACGAAAAAAACCTCTGCCTTATGAAAGCCATAGCATAGCTCCCAGAAAATCCCTGGGCATGCACTGGGA
TTCAAGAGTTGAGCCAAGGCTTCCACCAGAAGCTGAGAGGATTGCTATAGAACTTTTGGATGTAAGAGCC
TTTTGTTCAAACATTCCTCACTTAAAAGGAAAATCTGCTATGAAAAAGCGGCATTTGGAAATCTTGGGCT
ACCGTGTTATTCAGATCCCTTACTTTGAGTGGAACTCTATGGCAATGTCAACGAAGGATGCACGGATGGA
CTACCTGAGAGAACATCTATTTGGGGAAGGCAAATCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210852 protein sequence
Red=Cloning site Green=Tags(s)

MFRLRSISLLSWRAFPLRPFSCESLITQMQKCTNEEQVFDLIETNTATLSEQQVGCAFNVLWQFQKQKTV
LEKNVDHVRNHPQFLTLCSITTNHIPAMSDATLVDVLYSIKQFAVESHHPLIEALVTEAWKRLERFDTNV
LSIFSTCLADQHLYFSPLMGKIADIVNRRLETIQDLRALSVLMVSISSLISPCFQERLVIRTELLFDTVN
SSKVNIARRILLFLRNVKYSHYPLLERCNQVFIRNMSHLDLESISKILNLYQFLQFHSFEFVEAARGRLA
EMTLPSDHPESFVRLFAALGPVARPEIKKQLKSTILLLSEELSSQQALIVLGAMEDMESRNSHLLKKIVS
VLYKHLDNYKSIELLKIIQALTFLHFQSKELFMKLRELLLSRLEASVIPSEISVLVSALSMLPHPHLSET
AVSRIEAVLPQCDFRELNDLVVYLMRWIQSDLVCLASTTGKQLDLLQKLDQLGRHRLQQSTNLDLLWEEL
KSLKGEWLHESLVEESIAALLRFMDEIDYSNIAKVASFLSRTNYLNTLLLDRIASVAIQQVEKIHPFSVL
AIILPFSILNYDPPQKDEFFGACVQCCNSYLGTLDPGTLVFLGFSLAVLEYFPEDLLKKMFNIEFLARLD
SQLEILPSSLSARIQFRLMELNRAVCLECPELQVPWFHDRFCQRQFNKDTGVMNGAQQQIYKMLAEVLGG
HQCVKPSALSPYYHTVGFECILDKRKKPLPYESHSIAPRKSLGMHWDSRVEPRLPPEAERIAIELLDVRA
FCSNIPHLKGKSAMKKRHLEILGYRVIQIPYFEWNSMAMSTKDARMDYLREHLFGEGKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177244
ORF Size 2487 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177244.3, NP_796218.2
RefSeq Size 2877 bp
RefSeq ORF 2490 bp
Locus ID 320720
UniProt ID Q6DI86
Cytogenetics 2 C2
MW 95.4 kDa
Summary Involved in the down-regulation of mitochondrial MT-ND3 mRNA levels which leads to decreased respiratory complex I abundance and activity.UniProtKB/Swiss-Prot Function
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Documents
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"NM_177244" in other vectors (3)
SKU Description Size Price
MG210852 Fastkd1 (tGFP-tagged) - Mouse FAST kinase domains 1 (Fastkd1) 10 ug
$960.00
MR210852L3 Lenti ORF clone of Fastkd1 (Myc-DDK-tagged) - Mouse FAST kinase domains 1 (Fastkd1) 10 ug
$1,060.00
MR210852L4 Lenti ORF clone of Fastkd1 (mGFP-tagged) - Mouse FAST kinase domains 1 (Fastkd1) 10 ug
$1,060.00

Plasmid Map for MR210852

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210852
Circular map for MR210852

file-alt Citations

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