OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Vil1 (NM_009509) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR210849
Vil1 (Myc-DDK-tagged) - Mouse villin 1 (Vil1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $758.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Vil1 or NM_009509"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vil1
Synonyms Vil
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210849 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTAAACTGAATGCCCAAGTCAAAGGCTCTCTCAACATCACCACTCCCGGGATACAGATATGGAGGA
TCGAGGCTATGCAGATGGTACCTGTTCCTTCCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTATGT
AGTCCTGGCTATCCACAAGACCAGCAGCACTCTCTCCTATGATATCCACTACTGGATTGGCCAGGACTCG
TCCCAGGATGAGCAGGGGGCAGCTGCCATCTACACCACACAGATGGATGACTACCTGAAGGGCCGGGCTG
TCCAGCACCGCGAGGTTCAAGGCAACGAGAGCGAGACTTTCCGGAGCTACTTCAAGCAAGGCCTTGTGAT
CCGGAAAGGGGGAGTGGCTTCCGGCATGAAGCACGTAGAAACAAACTCCTGTGATGTCCAGCGACTGTTG
CACGTCAAGGGCAAGAGGAATGTGCTGGCTGGAGAGGTGGAAATGTCCTGGAAGAGTTTCAACAGAGGGG
ATGTCTTCCTGCTGGACCTTGGGAAGCTTATTATCCAGTGGAATGGGCCAGAGAGTAACCGCATGGAGAG
ACTTCGGGGCATGGCCTTGGCCAAAGAGATCCGAGACCAGGAACGGGGTGGACGTACCTACGTAGGTGTG
GTGGACGGGGAGAAGGAAGGGGACTCCCCACAGCTGATGGCAATTATGAACCACGTGCTGGGCCCACGCA
AAGAACTGAAGGCTGCTATTTCTGACTCAGTGGTGGAGCCGGCCGCTAAGGCTGCACTCAAGCTGTACCA
TGTGTCTGACTCCGAAGGAAAACTGGTGGTTAGAGAAGTTGCTACTCGGCCACTCACACAAGACCTGCTC
AAGCATGAGGACTGTTACATCCTGGACCAGGGAGGCCTGAAGATCTTTGTGTGGAAGGGAAAAAATGCCA
ACGCTCAGGAGAGGAGCGGAGCCATGAGCCAGGCCTTGAACTTCATCAAAGCCAAGCAGTACCCACCGAG
CACGCAGGTTGAGGTGCAGAATGACGGGGCCGAGTCCCCCATCTTCCAACAACTCTTCCAGAAGTGGACA
GTGCCCAACCGGACCTCAGGCCTCGGCAAAACCCACACTGTGGGCTCTGTGGCTAAGGTGGAACAGGTGA
AGTTTGATGCTCTGACCATGCATGTACAACCTCAGGTGGCTGCCCAGCAGAAAATGGTGGATGACGGGAG
TGGGGAAGTGCAGGTGTGGCGCATCGAGGACTTGGAGCTGGTGCCTGTGGAGTCCAAGTGGCTGGGCCAT
TTCTACGGTGGTGACTGCTACCTGCTGCTCTACACCTACCTCATAGGGGAAAAGCAACACTACTTGTTAT
ATATCTGGCAGGGCAGCCAGGCCAGCCAGGATGAAATTGCAGCCTCGGCGTATCAAGCCGTCCTGTTGGA
CCAGAAGTACAATGACGAGCCAGTACAGATCCGGGTCACGATGGGCAAGGAGCCGCCTCACCTCATGTCT
ATCTTCAAGGGCCGCATGGTGGTTTATCAGGGAGGCACCTCCCGAAAGAACAACTTGGAGCCTGTGCCCT
CTACGAGGCTATTTCAGGTCCGAGGGACCAATGCTGATAACACCAAGGCTTTTGAGGTGACAGCCCGGGC
CACGTCCCTCAACTCCAATGATGTCTTCATACTCAAGACTCCGTCCTGCTGCTACCTGTGGTGTGGGAAG
GGCTGCAGTGGGGATGAGAGGGAGATGGCCAAGATGGTTGCTGATACCATCTCTCGGACGGAGAAACAAG
TGGTAGTAGAGGGGCAGGAGCCAGCCAACTTCTGGATGGCTCTGGGCGGGAAGGCGCCCTACGCCAACAC
CAAGAGGCTGCAGGAGGAAAACCAAGTCATCACTCCTCGGCTCTTCGAGTGCTCCAACCAGACCGGACGC
TTTCTGGCCACAGAGATCTTTGACTTCAATCAGGATGACCTGGAGGAGGAGGATGTGTTCCTATTGGATG
TCTGGGACCAGGTCTTCTTCTGGATAGGGAAACATGCCAATGAGGAAGAGAAGAAGGCTGCAGCTACAAC
TGTACAAGAATACCTCAAGACCCACCCTGGAAACCGAGACCTTGAGACCCCTATCATCGTGGTGAAGCAG
GGACACGAGCCCCCCACCTTCACAGGCTGGTTCCTGGCTTGGGATCCCTTCAAGTGGAGTAACACCAAAT
CCTATGATGACCTTAAGGCAGAGCTGGGAAACTCTGGGGACTGGAGCCAGATTGCTGACGAGGTTATGAG
CCCGAAAGTGGACGTTTTCACTGCCAATACCAGTCTGAGTTCTGGGCCCCTGCCCACCTTCCCCCTGGAG
CAGCTGGTAAACAAGTCTGTAGAGGATCTCCCTGAGGGTGTGGACCCCAGCAGGAAGGAGGAGCACCTGT
CCACCGAAGACTTCACTAGGGCCTTGGGCATGACTCCAGCTGCCTTCTCTGCCCTGCCTCGATGGAAGCA
ACAAAACATCAAGAAAGAAAAAGGACTGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210849 protein sequence
Red=Cloning site Green=Tags(s)

MTKLNAQVKGSLNITTPGIQIWRIEAMQMVPVPSSTFGSFFDGDCYVVLAIHKTSSTLSYDIHYWIGQDS
SQDEQGAAAIYTTQMDDYLKGRAVQHREVQGNESETFRSYFKQGLVIRKGGVASGMKHVETNSCDVQRLL
HVKGKRNVLAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESNRMERLRGMALAKEIRDQERGGRTYVGV
VDGEKEGDSPQLMAIMNHVLGPRKELKAAISDSVVEPAAKAALKLYHVSDSEGKLVVREVATRPLTQDLL
KHEDCYILDQGGLKIFVWKGKNANAQERSGAMSQALNFIKAKQYPPSTQVEVQNDGAESPIFQQLFQKWT
VPNRTSGLGKTHTVGSVAKVEQVKFDALTMHVQPQVAAQQKMVDDGSGEVQVWRIEDLELVPVESKWLGH
FYGGDCYLLLYTYLIGEKQHYLLYIWQGSQASQDEIAASAYQAVLLDQKYNDEPVQIRVTMGKEPPHLMS
IFKGRMVVYQGGTSRKNNLEPVPSTRLFQVRGTNADNTKAFEVTARATSLNSNDVFILKTPSCCYLWCGK
GCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENQVITPRLFECSNQTGR
FLATEIFDFNQDDLEEEDVFLLDVWDQVFFWIGKHANEEEKKAAATTVQEYLKTHPGNRDLETPIIVVKQ
GHEPPTFTGWFLAWDPFKWSNTKSYDDLKAELGNSGDWSQIADEVMSPKVDVFTANTSLSSGPLPTFPLE
QLVNKSVEDLPEGVDPSRKEEHLSTEDFTRALGMTPAAFSALPRWKQQNIKKEKGLF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009509
ORF Size 2481 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009509.2
RefSeq Size 3117 bp
RefSeq ORF 2484 bp
Locus ID 22349
UniProt ID Q62468
Cytogenetics 1 38.54 cM
MW 92.8 kDa
Summary Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009509" in other vectors (4)
SKU Description Size Price
MC201154 Vil1 (untagged) - Mouse villin 1 (Vil1), (10ug) 10 ug
$758.00
MG210849 Vil1 (tGFP-tagged) - Mouse villin 1 (Vil1) 10 ug
$958.00
MR210849L3 Lenti ORF clone of Vil1 (Myc-DDK-tagged) - Mouse villin 1 (Vil1) 10 ug
$1,058.00
MR210849L4 Lenti ORF clone of Vil1 (mGFP-tagged) - Mouse villin 1 (Vil1) 10 ug
$1,058.00

Plasmid Map for MR210849

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210849
Circular map for MR210849

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products