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Arel1 (NM_178065) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210828
1110018G07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110018G07 gene (1110018G07Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $754.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Arel1 or NM_178065"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arel1
Synonyms 1110018G07Rik; AI649076; AW610792; Kiaa0317; mKIAA0317
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210828 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTACGTTATTGGTGGAATCATAGTGTCTGTGGTTGCATTCTTCTTCACAATTAAGTTCCTCTTTG
AGCTTGCCGCACGTGTAGTCAGCTTTCTTCAGAATGAAGACCGTGAGCGCCGAGGGGACCGGACTATTTA
TGACTACGTGCGGGGAAATTACCTGGATCCCCGGTCCTGCAAAGTGTCCTGGGATTGGAAGGACCCCTAT
GAGGTGGGCCACAGCATGGCCTTCCGAGTGCATTTGTTCTATAAGAATGGACAGCCTTTCCCTGCACATC
GGCCCGTGGGACTGCGGGTTCACATCTCTCACGTTGAACTAGCAGTGGACATTCCAGTGACCCAGGAAGT
CCTCCAGGAGCCGAATTCTAACGTGGTGAAGGTGGCCTTCACTGTGCGCAAGGCTGGGCGCTATGAGATC
ACTGTGAAGTTGGGTGGCTTAAATGTGGCCTATAGTCCCTACTACAAAATTTTTCAGCCTGGAATGGTGG
TTCCTTCCAAAACAAAAATTGTGTGCCATTTTTCTACTCTGGTGTTGACGTGTGGGCAGCCACACACTCT
GCAGATAGTGCCCCGAGATGAGTATGACAACCCAACCAACAATTCCATGTCCCTGAGAGATGAGCACAGC
TACAGCTTAGCCATTCACGAGCTTGGTCCTCAAGAAGAAGAGAATAATGAAGTCTCATTTGAGAAGTCAG
TGACGTCCAACAGGCAGACTTGCCAGGTGTTCTTGCGGCTCACCCTGCATTCCCGAGGTTGCTTCCATGC
CTGCATTTCATATCAGAACCAACCCATCAATAATGGCGAATTTGACATTATTGTCCTTAGTGAGAATGAG
AAGAATATTGTTGAACGAAATGTGTCCACCTCAGGAGTGAGCATTTACTTTGAGGCTTACCTTTACAATG
CTAACAACTGTACCAGCACACCATGGCATCTTCCTCCCATGCACATGAGCTCTTCTCAACGCCGGCCCTC
CACTGCTATTGAGGAGGACGATGAAGACTCACCCTCAGAGTGTCACACCCCTGAGAAGGTGAAGAAACCG
AAGAAGGTGTACTGCTATGTGTCACCAAAGCAATTCTCTGTGAAGGAATTCTACCTGAAAATCATCCCCT
GGCGCCTCTACACCTTCCGAGTGTGCCCAGGAACAAAGTTTTCATACCTTGGCCCTGACCCTGTTCACAA
GCTCCTCACGCTGGTGGTGGATGACGGCATTCAGCCTCCAGTGGAGCTCAGCTGTAAAGAAAGGAATATC
CTAGCAGCCATTTTCATCCGTTCCTTGCACAAGAACATTGGAGGCTCTGAGACCTTTCAAGACAAGGTGA
ACTTTTTCCAGAGAGAGCTTCGGCAGGTACATATGAAGAGACCTCATTCCAAAGTCACTCTGAAGGTCAG
CAGACACGCCTTGTTGGAGTCGTCTTTGAAGGCCACTCGGAACTTTTCCATCTCAGACTGGAGCAAGAAC
TTTGAAGTAGTTTTCCAGGATGAGGAAGCTTTGGATTGGGGAGGGCCTCGCCGGGAATGGTTTGAGCTAA
TCTGCAAAGCACTGTTTGACACCACCAGTCAGCTCTTCGCCCGGTTCACTGACAGCAACCAAGCACTAGT
GCATCCTAACCCTAACCGCCCGGCTCACCTGCGCTTGAAGATGTATGAGTTTGCAGGGCGGCTGGTGGGC
AAGTGTTTGTATGAATCCTCTCTAGGGGGAGCCTACAAGCAGTTGGTCCGTGCCCGGTTCACTCGGTCTT
TCCTGGCCCAAATCATAGGACTCCGTATGCATTACAAGTACTTTGAAACGGATGACCCAGAATTCTATAA
GTCCAAGGTGTGCTTTATCCTCAACAATGACATGAGTGAGATGGAGCTGGTCTTTGCAGAAGAGAAATAC
AACAAATCAGGTCAACTGGATAAGATTGTAGAACTCATGACAGGCGGAGCTCAAACCCCAGTCACCAATG
CAAATAAAATCTTCTATTTAAACCTGCTGGCTCAGTATCGGCTGGCCAGTCAAGTGAAAGAGGAGGTGGA
GCATTTCCTGAAAGGCTTGAATGAGCTGGTTCCTGAGAACCTCCTGGCTATTTTTGATGAGAATGAGCTT
GAGCTGCTGATGTGTGGGACTGGAGACATCAATGTATCTGACTTCAAAGCCCATGCTGTAGTGGTTGGTG
GATCCTGGCATTTCAGAGAAAAGGTCATGAGGTGGTTTTGGGCTGTGGTTTCCAGTCTGACCCAGGAGGA
ACTGGCTAGGCTGCTTCAGTTTACAACAGGCTCTTCTCAGCTCCCACCTGGAGGATTTGCTGCCCTCTGT
CCCTCATTTCAGATTATTGCTGCTCCAACCCATAGCACGCTTCCTACTGCACACACATGTTTTAACCAAC
TGTGCCTCCCTACATATGACTCATATGAAGAGGTGCACAGGATGCTACAGCTGGCCATCAGTGAGGGTTG
CGAGGGCTTTGGCATGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210828 protein sequence
Red=Cloning site Green=Tags(s)

MFYVIGGIIVSVVAFFFTIKFLFELAARVVSFLQNEDRERRGDRTIYDYVRGNYLDPRSCKVSWDWKDPY
EVGHSMAFRVHLFYKNGQPFPAHRPVGLRVHISHVELAVDIPVTQEVLQEPNSNVVKVAFTVRKAGRYEI
TVKLGGLNVAYSPYYKIFQPGMVVPSKTKIVCHFSTLVLTCGQPHTLQIVPRDEYDNPTNNSMSLRDEHS
YSLAIHELGPQEEENNEVSFEKSVTSNRQTCQVFLRLTLHSRGCFHACISYQNQPINNGEFDIIVLSENE
KNIVERNVSTSGVSIYFEAYLYNANNCTSTPWHLPPMHMSSSQRRPSTAIEEDDEDSPSECHTPEKVKKP
KKVYCYVSPKQFSVKEFYLKIIPWRLYTFRVCPGTKFSYLGPDPVHKLLTLVVDDGIQPPVELSCKERNI
LAAIFIRSLHKNIGGSETFQDKVNFFQRELRQVHMKRPHSKVTLKVSRHALLESSLKATRNFSISDWSKN
FEVVFQDEEALDWGGPRREWFELICKALFDTTSQLFARFTDSNQALVHPNPNRPAHLRLKMYEFAGRLVG
KCLYESSLGGAYKQLVRARFTRSFLAQIIGLRMHYKYFETDDPEFYKSKVCFILNNDMSEMELVFAEEKY
NKSGQLDKIVELMTGGAQTPVTNANKIFYLNLLAQYRLASQVKEEVEHFLKGLNELVPENLLAIFDENEL
ELLMCGTGDINVSDFKAHAVVVGGSWHFREKVMRWFWAVVSSLTQEELARLLQFTTGSSQLPPGGFAALC
PSFQIIAAPTHSTLPTAHTCFNQLCLPTYDSYEEVHRMLQLAISEGCEGFGML

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178065
ORF Size 2469 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178065.3, NP_835166.2
RefSeq Size 5476 bp
RefSeq ORF 2472 bp
Locus ID 68497
UniProt ID Q8CHG5
Cytogenetics 12 D1
MW 94.2 kDa
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits apoptosis by ubiquitinating and targeting for degradation a number of proapoptotic proteins including DIABLO/SMAC, HTRA2 and SEPT4/ARTS which are released from the mitochondrion into the cytosol following apoptotic stimulation.UniProtKB/Swiss-Prot Function
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Resources
"NM_178065" in other vectors (4)
SKU Description Size Price
MC221874 Arel1 (untagged) - Mouse RIKEN cDNA 1110018G07 gene (1110018G07Rik), (10ug) 10 ug
$830.00
MG210828 1110018G07Rik (tGFP-tagged) - Mouse RIKEN cDNA 1110018G07 gene (1110018G07Rik) 10 ug
$789.00 $954.00
MR210828L3 Lenti ORF clone of 1110018G07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1110018G07 gene (1110018G07Rik) 10 ug
$1,054.00
MR210828L4 Lenti ORF clone of 1110018G07Rik (mGFP-tagged) - Mouse RIKEN cDNA 1110018G07 gene (1110018G07Rik) 10 ug
$1,054.00

Plasmid Map for MR210828

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210828
Circular map for MR210828

file-alt Citations

IL-1β turnover by the UBE2L3 ubiquitin conjugating enzyme and HECT E3 ligases limits inflammation
Mishra, Crespo-Puig, McCarthy et al
Nat Commun (2023) 14 (1), 4385 DOI: 10.1038/s41467-023-40054-x
Cited by 22
AI Summary: OriGene’s mouse AREL1 cDNA (MR210828) was used to generate expression constructs for investigating the role of AREL1 in pro-IL-1β ubiquitylation and turnover in this study.
Snippet: 2L3 plasmids were described before6. Similarly, pro-IL-1β constructs with 2x C-terminal StrepTag II tags were cloned into pMX-CMV plasmid for constitutive expression. Mouse AREL1 cDNA obtained from Origene (MR210828) and the different constructs (HECT-domain: aa 436-823) were cloned with two C-terminal 2x-Myc tags or N-terminal mVenus and C-terminal 2x-Myc tags into pMX-CMV-mVenus-2Myc via PCR
FigureFigureFigure
Showing 1-1 of 1 papers.
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