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Elfn2 (NM_183141) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210827
Elfn2 (Myc-DDK-tagged) - Mouse leucine rich repeat and fibronectin type III, extracellular 2 (Elfn2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $754.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Elfn2
Synonyms 6330514E13; AW048948; BC094219; Lrrc62; Ppp1r29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210827 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGCCTGGGGCTGTGTGCGGCAGCGCTGCTGTGCGTGTGCCAGCCAGGGGCTGTGCGCGCTGACT
GCTGGCTCATCGAGGGGGACAAGGGCTACGTGTGGCTGGCCATCTGCAGCCAGAACCAGCCTCCATATGA
GACCATCCCTCAGCACATCAACAGCACTGTGCATGACCTGCGGCTCAATGAAAACAAGCTCAAGGCCGTG
CTCTATTCCTCCCTCAACCGCTTCGGGAACCTCACGGACCTCAACCTCACCAAGAACGAGATTTCCTACA
TCGAGGATGGTGCCTTCCTGGGCCAGACGAGCCTGCAGGTCCTGCAGCTGGGCTACAACCGGCTCAGCAA
CCTGACTGAGGGCATGCTGCGAGGTATGAGCCGCCTGCAGTTCCTCTTCGTCCAGCACAACCTCATCGAG
GTGGTGACGCCCACCGCCTTCTCCGAGTGCCCCAGTCTCATCAGCATCGACCTCTCCTCCAACCGCCTCA
GCCGCCTGGACGGCGCGACCTTCGCCAGCCTGGCCAGCCTGATGGTGTGCGAGCTGGCTGGCAACCCCTT
CAACTGTGAGTGTGACCTCTTCGGCTTCCTAGCCTGGCTTGTGGTCTTCAACAATGTCACCAAGAACTAT
GATCGCCTGCAGTGTGAGTCACCGAGGGAGTTTGCCGGTTACCCGCTGCTCGTGCCCAGACCTTACCACA
GCCTCAATGCCATCACTGTCCTGCAGGCCAAGTGCCGCAACGGCTCAATGCCTGCCCGGCCTGTGAGTCA
CCCCACACCCTATTCCACGGATGCGCAGAGGGAGCCTGACGAGAACTCAGGCTTCAATCCTGACGAGATC
CTCTCGGTGGAGCCGCCCGCCTCCTCCACCACAGATGCATCTGCTGGGCCGGCCATCAAGCTGCACCAAG
TCACCTTCACCTCGGCCACCCTGGTTGTCATCATCCCCCACCCTTACAGCAAGATGTATGTTCTGGTCCA
GTACAACAATAGCTACTTCTCTGACGTCATGACACTCAAGAACAAGAAAGAGATTGTGACGTTGGACAAG
CTGCGAGCGCACACTGAGTACACCTTCTGTGTCACCTCGCTGCGCAACAGCCGCCGCTTCAACCACACCT
GCCTGACCTTCACCACGCGGGACCTCGTGCCCGGGGATCTGGCCCCCAGCACCTCCACCACCACGCACTA
CATCATGACTATCCTGGGCTGCCTGTTTGGCATGGTCATCGTGCTGGGGGCTGTCTACTACTGCCTGCGG
AAGCGGCGCATGCAAGAGGAGAAGCAGAAGTCCGTCAATGTTAAGAAGACTATCTTAGAGATGCGCTATG
GCGCTGACGTGGATGCCGGTTCCATAGTGCACGCTGCACAGAAGCTGGGCGAGCCACCGGTGCTGCCCGT
GGCCAGAATGTCCTCTATTCCTTCCATGGTCGGAGAGAAACTGCCTGCCTCCAAGGGTCTGGAGGCTGGG
CTAGACACACCCAAGGTGGCTACCAAAGGCAACTATATTGAAGTGCGCACAGGTGCTGCAGGAGACAGCC
TGGCCCGGCCTGAGGAGGAGCTTCCAGAAATCGAAAATGGCCAGGGCTCAGCGGCTGAGATCTCCACCAT
TGCCAAGGAGGTGGACAAGGTCAACCAGATCATTAACAATTGCATCGATGCCCTCAAGCTGGATTCAGCC
TCTTTCCTTGGGGGTGGCGGCGGCGGTGGAGGCGGCGGGGACTCCGATCTGGCCTTCGAGTGCCAGTCCC
TCCCTGCGGCTCCTGCTGCCTCCTCGGCTGCCACTCCCGGGGCGCTGGAGCGGCCCAGCTTCCTGTCACC
CCCCTACAAGGAGAGCTCCCACCACCCGCTGCAGCGCCAGCTGAGCGCCGACGCCGCAGTGAGCCGTAAG
ACCTGCAGCGTGTCGTCCAGTGGCTCCATCAAGAGCGCCAAGGTCTTCAGTCTGGATGTGCCAGACCACC
CGACCCCCACGGGACTGGCTAAGAGCGACTCCAAGTACATTGAGAAAGGCAGCCCCCTCAACAGCCCGCT
GGACAGGCTTCCCCTGGTGCCCACGGGCAGCAGCGGGAGCAGTGGTGGGGGTGGTGGCATCCACCACCTG
GAGGTGAAGCCGGCCTACCACTGCAGCGAGCACCGGCACAGCTTCCCCGCTCTCTACTACGAGGAGGGCG
CCGACAGCCTGAGCCAGCGTGTGTCCTTCCTCAAGCCACTGACCCGCTCTAAGCGAGACTCCACCTATTC
GCAGCTCTCCCCCAGACATTACTACTCAGGGTACTCCTCCAGCCCCGAGTACTCGTCTGAGAGCACACAT
AAGATCTGGGAGCGCTTCCGGCCCTACAAGAAACACCATCGCGAGGAGGTATACATGGCTGCCGGCCACG
CCCTGCGTAAGAAGGTCCAGTTCGCGAAGGATGAGGACCTGCATGACATCCTTGATTACTGGAAGGGGGT
CTCAGCCCAGCAGAAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210827 protein sequence
Red=Cloning site Green=Tags(s)

MLRLGLCAAALLCVCQPGAVRADCWLIEGDKGYVWLAICSQNQPPYETIPQHINSTVHDLRLNENKLKAV
LYSSLNRFGNLTDLNLTKNEISYIEDGAFLGQTSLQVLQLGYNRLSNLTEGMLRGMSRLQFLFVQHNLIE
VVTPTAFSECPSLISIDLSSNRLSRLDGATFASLASLMVCELAGNPFNCECDLFGFLAWLVVFNNVTKNY
DRLQCESPREFAGYPLLVPRPYHSLNAITVLQAKCRNGSMPARPVSHPTPYSTDAQREPDENSGFNPDEI
LSVEPPASSTTDASAGPAIKLHQVTFTSATLVVIIPHPYSKMYVLVQYNNSYFSDVMTLKNKKEIVTLDK
LRAHTEYTFCVTSLRNSRRFNHTCLTFTTRDLVPGDLAPSTSTTTHYIMTILGCLFGMVIVLGAVYYCLR
KRRMQEEKQKSVNVKKTILEMRYGADVDAGSIVHAAQKLGEPPVLPVARMSSIPSMVGEKLPASKGLEAG
LDTPKVATKGNYIEVRTGAAGDSLARPEEELPEIENGQGSAAEISTIAKEVDKVNQIINNCIDALKLDSA
SFLGGGGGGGGGGDSDLAFECQSLPAAPAASSAATPGALERPSFLSPPYKESSHHPLQRQLSADAAVSRK
TCSVSSSGSIKSAKVFSLDVPDHPTPTGLAKSDSKYIEKGSPLNSPLDRLPLVPTGSSGSSGGGGGIHHL
EVKPAYHCSEHRHSFPALYYEEGADSLSQRVSFLKPLTRSKRDSTYSQLSPRHYYSGYSSSPEYSSESTH
KIWERFRPYKKHHREEVYMAAGHALRKKVQFAKDEDLHDILDYWKGVSAQQKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183141
ORF Size 2469 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183141.3
RefSeq Size 4877 bp
RefSeq ORF 2472 bp
Locus ID 207393
UniProt ID Q68FM6
Cytogenetics 15 E1
MW 90 kDa
Summary Inhibits phosphatase activity of protein phosphatase 1 (PP1) complexes.UniProtKB/Swiss-Prot Function
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"NM_183141" in other vectors (3)
SKU Description Size Price
MG210827 Elfn2 (tGFP-tagged) - Mouse 5leucine rich repeat containing 62 (Lrrc62) 10 ug
$954.00
MR210827L3 Lenti ORF clone of Elfn2 (Myc-DDK-tagged) - Mouse leucine rich repeat and fibronectin type III, extracellular 2 (Elfn2) 10 ug
$1,054.00
MR210827L4 Lenti ORF clone of Elfn2 (mGFP-tagged) - Mouse leucine rich repeat and fibronectin type III, extracellular 2 (Elfn2) 10 ug
$1,054.00

Plasmid Map for MR210827

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file-download TXT (FASTA format)

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Cloning scheme for MR210827
Circular map for MR210827

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